In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed Polyphenols
Flaxseed is an oilseed (45–50% oil on a dry-weight basis) crop. Its oil has demonstrated multiple health benefits and industrial applications. The goal of this research was to evaluate the antidiabetic and anti-inflammatory potential of the free polyphenol fraction of flax (<i>Linum usitatissi...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2072-6643/13/8/2759 |
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| author | Hamza Mechchate Imane Es-safi Raffaele Conte Christophe Hano Amal Amaghnouje Fatima Zahra Jawhari Nabil Radouane Noureddine Bencheikh Andriy Grafov Dalila Bousta |
| author_facet | Hamza Mechchate Imane Es-safi Raffaele Conte Christophe Hano Amal Amaghnouje Fatima Zahra Jawhari Nabil Radouane Noureddine Bencheikh Andriy Grafov Dalila Bousta |
| author_sort | Hamza Mechchate |
| collection | DOAJ |
| description | Flaxseed is an oilseed (45–50% oil on a dry-weight basis) crop. Its oil has demonstrated multiple health benefits and industrial applications. The goal of this research was to evaluate the antidiabetic and anti-inflammatory potential of the free polyphenol fraction of flax (<i>Linum usitatissimum</i> L.) seeds (PLU), based on their use in traditional medicine. Mice with alloxan-induced diabetes were used to study the antidiabetic activity of PLU in vivo, with an oral administration of 25 and 50 mg/kg over 28 days. Measurements of body weight and fasting blood glucose (FBG) were carried out weekly, and biochemical parameters were evaluated. An oral glucose tolerance test was also performed. Inhibitory activities of PLU on α-amylase and α-glucosidase activities were evaluated in vitro. The anti-inflammatory was evaluated in vivo in Wistar rats using the paw edema induction Test by carrageenan, and in vitro using the hemolysis ratio test. PLU administration to diabetic mice during the study period improved their body weight and FBG levels remarkably. In vitro inhibitory activity of digestive enzymes indicated that they may be involved in the proposed mode of action of PLU extract. Qualitative results of PLU revealed the presence of 18 polyphenols. These findings support daily consumption of flaxseed for people with diabetes, and suggest that polyphenols in flaxseed may serve as dietary supplements or novel phytomedicines to treat diabetes and its complications. |
| first_indexed | 2024-03-10T08:30:29Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T08:30:29Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-afd3e633cc334f10bb4dfbe4725f03b02023-11-22T09:05:41ZengMDPI AGNutrients2072-66432021-08-01138275910.3390/nu13082759In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed PolyphenolsHamza Mechchate0Imane Es-safi1Raffaele Conte2Christophe Hano3Amal Amaghnouje4Fatima Zahra Jawhari5Nabil Radouane6Noureddine Bencheikh7Andriy Grafov8Dalila Bousta9Laboratory of Biotechnology, Environment, Agri-Food, and Health, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, P.O. Box 1796, Fez 30000, MoroccoLaboratory of Biotechnology, Environment, Agri-Food, and Health, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, P.O. Box 1796, Fez 30000, MoroccoResearch Institute on Terrestrial Ecosystems (IRET)—CNR, Via Pietro Castellino 111, 80131 Naples, ItalyLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, Campus Eure et Loir, Orleans University, 45067 Orleans, FranceLaboratory of Biotechnology, Environment, Agri-Food, and Health, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, P.O. Box 1796, Fez 30000, MoroccoLaboratory of Biotechnology, Environment, Agri-Food, and Health, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, P.O. Box 1796, Fez 30000, MoroccoLaboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Fez 30000, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda 60000, MoroccoLaboratory of Inorganic Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, FI-00014 Helsinki, FinlandLaboratory of Biotechnology, Environment, Agri-Food, and Health, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, P.O. Box 1796, Fez 30000, MoroccoFlaxseed is an oilseed (45–50% oil on a dry-weight basis) crop. Its oil has demonstrated multiple health benefits and industrial applications. The goal of this research was to evaluate the antidiabetic and anti-inflammatory potential of the free polyphenol fraction of flax (<i>Linum usitatissimum</i> L.) seeds (PLU), based on their use in traditional medicine. Mice with alloxan-induced diabetes were used to study the antidiabetic activity of PLU in vivo, with an oral administration of 25 and 50 mg/kg over 28 days. Measurements of body weight and fasting blood glucose (FBG) were carried out weekly, and biochemical parameters were evaluated. An oral glucose tolerance test was also performed. Inhibitory activities of PLU on α-amylase and α-glucosidase activities were evaluated in vitro. The anti-inflammatory was evaluated in vivo in Wistar rats using the paw edema induction Test by carrageenan, and in vitro using the hemolysis ratio test. PLU administration to diabetic mice during the study period improved their body weight and FBG levels remarkably. In vitro inhibitory activity of digestive enzymes indicated that they may be involved in the proposed mode of action of PLU extract. Qualitative results of PLU revealed the presence of 18 polyphenols. These findings support daily consumption of flaxseed for people with diabetes, and suggest that polyphenols in flaxseed may serve as dietary supplements or novel phytomedicines to treat diabetes and its complications.https://www.mdpi.com/2072-6643/13/8/2759flaxflaxseedpolyphenols extractiondiabetes mellitusantidiabeticanti-inflammatory |
| spellingShingle | Hamza Mechchate Imane Es-safi Raffaele Conte Christophe Hano Amal Amaghnouje Fatima Zahra Jawhari Nabil Radouane Noureddine Bencheikh Andriy Grafov Dalila Bousta In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed Polyphenols Nutrients flax flaxseed polyphenols extraction diabetes mellitus antidiabetic anti-inflammatory |
| title | In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed Polyphenols |
| title_full | In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed Polyphenols |
| title_fullStr | In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed Polyphenols |
| title_full_unstemmed | In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed Polyphenols |
| title_short | In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (<i>Linum usitatissimum</i> L.) Seed Polyphenols |
| title_sort | in vivo and in vitro antidiabetic and anti inflammatory properties of flax i linum usitatissimum i l seed polyphenols |
| topic | flax flaxseed polyphenols extraction diabetes mellitus antidiabetic anti-inflammatory |
| url | https://www.mdpi.com/2072-6643/13/8/2759 |
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