Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-I
Nascent HDL is known to be formed by the interaction of apolipoprotein A-I (apoA-I) with transmembrane ABCA1, but the molecular mechanism by which nascent HDL forms is less well understood. Here, we studied how reconstituted high density lipoprotein (rHDL) forms spontaneously on the interaction of a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2007-04-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520434418 |
| _version_ | 1818728214742171648 |
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| author | Masakazu Fukuda Minoru Nakano Supaporn Sriwongsitanont Masaharu Ueno Yoshihiro Kuroda Tetsurou Handa |
| author_facet | Masakazu Fukuda Minoru Nakano Supaporn Sriwongsitanont Masaharu Ueno Yoshihiro Kuroda Tetsurou Handa |
| author_sort | Masakazu Fukuda |
| collection | DOAJ |
| description | Nascent HDL is known to be formed by the interaction of apolipoprotein A-I (apoA-I) with transmembrane ABCA1, but the molecular mechanism by which nascent HDL forms is less well understood. Here, we studied how reconstituted high density lipoprotein (rHDL) forms spontaneously on the interaction of apoA-I with model membranes. The formation of rHDL from pure phosphatidylcholine (PC) large unilamellar vesicles (LUVs) proceeded very slowly at 37.0°C, but sphingomyelin (SM) -rich PC/SM LUVs, which are in a gel/liquid-disordered phase (Ld phase) at this temperature, were rapidly microsolubilized to form rHDL by apoA-I. The addition of cholesterol decreased the rate at which rHDL formed and induced the selective extraction of lipids by apoA-I, which preferably extracted lipids of Ld phase rather than lipids of liquid-ordered phase. In addition, apoA-I extracted lipids from the outer and inner leaflets of LUVs simultaneously. These results suggest that the heterogeneous interface of the mixed membranes facilitates the insertion of apoA-I and induces Ld phase-selective but leaflet-nonselective lipid extraction to form rHDL; they are compatible with recent cell works on apoA-I-dependent HDL generation. |
| first_indexed | 2024-12-17T22:26:26Z |
| format | Article |
| id | doaj.art-afd5b7cf065445db8d0b8b812c2e9d74 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T22:26:26Z |
| publishDate | 2007-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-afd5b7cf065445db8d0b8b812c2e9d742022-12-21T21:30:19ZengElsevierJournal of Lipid Research0022-22752007-04-01484882889Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-IMasakazu Fukuda0Minoru Nakano1Supaporn Sriwongsitanont2Masaharu Ueno3Yoshihiro Kuroda4Tetsurou Handa5Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, JapanGraduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, JapanFaculty of Pharmaceutical Sciences, Toyama University, 2630 Sugitani, Toyama 930-0194, JapanFaculty of Pharmaceutical Sciences, Toyama University, 2630 Sugitani, Toyama 930-0194, JapanGraduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, JapanGraduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, JapanNascent HDL is known to be formed by the interaction of apolipoprotein A-I (apoA-I) with transmembrane ABCA1, but the molecular mechanism by which nascent HDL forms is less well understood. Here, we studied how reconstituted high density lipoprotein (rHDL) forms spontaneously on the interaction of apoA-I with model membranes. The formation of rHDL from pure phosphatidylcholine (PC) large unilamellar vesicles (LUVs) proceeded very slowly at 37.0°C, but sphingomyelin (SM) -rich PC/SM LUVs, which are in a gel/liquid-disordered phase (Ld phase) at this temperature, were rapidly microsolubilized to form rHDL by apoA-I. The addition of cholesterol decreased the rate at which rHDL formed and induced the selective extraction of lipids by apoA-I, which preferably extracted lipids of Ld phase rather than lipids of liquid-ordered phase. In addition, apoA-I extracted lipids from the outer and inner leaflets of LUVs simultaneously. These results suggest that the heterogeneous interface of the mixed membranes facilitates the insertion of apoA-I and induces Ld phase-selective but leaflet-nonselective lipid extraction to form rHDL; they are compatible with recent cell works on apoA-I-dependent HDL generation.http://www.sciencedirect.com/science/article/pii/S0022227520434418ATP binding cassette transporter A1cholesterollipid effluxliposomeliquid-disordered phaseliquid-ordered phase |
| spellingShingle | Masakazu Fukuda Minoru Nakano Supaporn Sriwongsitanont Masaharu Ueno Yoshihiro Kuroda Tetsurou Handa Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-I Journal of Lipid Research ATP binding cassette transporter A1 cholesterol lipid efflux liposome liquid-disordered phase liquid-ordered phase |
| title | Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-I |
| title_full | Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-I |
| title_fullStr | Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-I |
| title_full_unstemmed | Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-I |
| title_short | Spontaneous reconstitution of discoidal HDL from sphingomyelin-containing model membranes by apolipoprotein A-I |
| title_sort | spontaneous reconstitution of discoidal hdl from sphingomyelin containing model membranes by apolipoprotein a i |
| topic | ATP binding cassette transporter A1 cholesterol lipid efflux liposome liquid-disordered phase liquid-ordered phase |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520434418 |
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