Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effects
There is an increasing and unfulfilled demand of bone substitutes with optimal mechanical and biological properties. Based on the excellent mechanical and structural properties of Portland Cement, a biocompatibility exploratory study of this material was proposed. Plain substrates were fabricated w...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2006-08-01
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| Series: | Revista Facultad de Ingeniería Universidad de Antioquia |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/ingenieria/article/view/343394 |
| _version_ | 1827981309765484544 |
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| author | Daniel Gallego Luis Ernesto López Derek Hansford Jonas Klemas |
| author_facet | Daniel Gallego Luis Ernesto López Derek Hansford Jonas Klemas |
| author_sort | Daniel Gallego |
| collection | DOAJ |
| description |
There is an increasing and unfulfilled demand of bone substitutes with optimal mechanical and biological properties. Based on the excellent mechanical and structural properties of Portland Cement, a biocompatibility exploratory study of this material was proposed. Plain substrates were fabricated with Gray Type I Portland Cement under different conditions (Neutralized-SN, Carbonated-SC, not neutralized-SnN), which were then used to conduct a Direct Contact Assay with CHO and HOS cells for 24h. The substrates were characterized by SEM, and phenolphthalein assays to determine the pH value, while the cell culture assays were evaluated by Phase Contrast Microscopy. The results show that SnN had the highest pH value (> 12,0), followed by SN, and finally by SC (≈ 7,4); it was also observed that the cytotoxicity of the substrates diminished in proportion to the pH value. It is proposed that the cytotoxicity of Portland Cement is caused by the Ca(OH)2 formed during the hydration of this material. Thus, by lowering the amount of Ca(OH)2, either by carbonation or neutralization, the biocompatibility of the material is positively affected.
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| first_indexed | 2024-04-09T22:06:24Z |
| format | Article |
| id | doaj.art-afd6f6ae14594fb7b4d030b16f911541 |
| institution | Directory Open Access Journal |
| issn | 0120-6230 2422-2844 |
| language | English |
| last_indexed | 2024-04-09T22:06:24Z |
| publishDate | 2006-08-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Revista Facultad de Ingeniería Universidad de Antioquia |
| spelling | doaj.art-afd6f6ae14594fb7b4d030b16f9115412023-03-23T12:38:12ZengUniversidad de AntioquiaRevista Facultad de Ingeniería Universidad de Antioquia0120-62302422-28442006-08-013710.17533/udea.redin.343394Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effectsDaniel Gallego0Luis Ernesto López1 Derek Hansford2Jonas Klemas3Escuela de Ingeniería de AntioquiaEscuela de Ingeniería de AntioquiaThe Ohio State UniversityThe Ohio State University There is an increasing and unfulfilled demand of bone substitutes with optimal mechanical and biological properties. Based on the excellent mechanical and structural properties of Portland Cement, a biocompatibility exploratory study of this material was proposed. Plain substrates were fabricated with Gray Type I Portland Cement under different conditions (Neutralized-SN, Carbonated-SC, not neutralized-SnN), which were then used to conduct a Direct Contact Assay with CHO and HOS cells for 24h. The substrates were characterized by SEM, and phenolphthalein assays to determine the pH value, while the cell culture assays were evaluated by Phase Contrast Microscopy. The results show that SnN had the highest pH value (> 12,0), followed by SN, and finally by SC (≈ 7,4); it was also observed that the cytotoxicity of the substrates diminished in proportion to the pH value. It is proposed that the cytotoxicity of Portland Cement is caused by the Ca(OH)2 formed during the hydration of this material. Thus, by lowering the amount of Ca(OH)2, either by carbonation or neutralization, the biocompatibility of the material is positively affected. https://revistas.udea.edu.co/index.php/ingenieria/article/view/343394biocompatibilitycalcium carbonatecarbonationcytotoxicitydirect contact |
| spellingShingle | Daniel Gallego Luis Ernesto López Derek Hansford Jonas Klemas Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effects Revista Facultad de Ingeniería Universidad de Antioquia biocompatibility calcium carbonate carbonation cytotoxicity direct contact |
| title | Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effects |
| title_full | Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effects |
| title_fullStr | Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effects |
| title_full_unstemmed | Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effects |
| title_short | Potential applications of portland cement on bone tissue engineering. Phase I: biocompatibility studies–calcium hydroxide effects |
| title_sort | potential applications of portland cement on bone tissue engineering phase i biocompatibility studies calcium hydroxide effects |
| topic | biocompatibility calcium carbonate carbonation cytotoxicity direct contact |
| url | https://revistas.udea.edu.co/index.php/ingenieria/article/view/343394 |
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