PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPY
Objective. Mitochondrial injury plays a central role in neuronal death following ischemic stroke. In the present study, we investigated effects of ethylmethylhydroxypyridine succinate on a microstroke-induced mitochondria swelling, a hallmark of mitochondrial injury.Materials and Methods. Ischemic m...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
IRBIS LLC
2016-08-01
|
| Series: | Эпилепсия и пароксизмальные состояния |
| Subjects: | |
| Online Access: | https://www.epilepsia.su/jour/article/view/228 |
| _version_ | 1797879466038394880 |
|---|---|
| author | R. A. Afzalov Yu. O. Kolikova E. G. Pryazhnikov D. V. Toptunov L. S. Khirug |
| author_facet | R. A. Afzalov Yu. O. Kolikova E. G. Pryazhnikov D. V. Toptunov L. S. Khirug |
| author_sort | R. A. Afzalov |
| collection | DOAJ |
| description | Objective. Mitochondrial injury plays a central role in neuronal death following ischemic stroke. In the present study, we investigated effects of ethylmethylhydroxypyridine succinate on a microstroke-induced mitochondria swelling, a hallmark of mitochondrial injury.Materials and Methods. Ischemic microstroke was induced in Thy1-CFP-MitoS mice expressing cyano fluorescent protein (CFP) in brain mitochondria by impulse infrared laser. Ethylmethylhydroxypyridine succinate 25 mg/kg or saline (control) were administered i.p. at 30 min after the stroke onset. A period of observation was 48 h. Brain images were obtained by two photon laser fluorescent microscopy and analyzed using a software developed in Neurotar Ltd (Finland). Nonparametric Mann-Whitney-Wilcoxon test was used for te statistical analysis of results.Results. Microstroke resulted in mitochondria swelling, i.e. injury, in the zone surrounding the thrombus. The most profound changes of mitochondrial morphology were observed at 2 h from the stroke onset. Ethylmethylhydroxypyridine succinate significantly reduces the stroke-induced swelling at 1 h (p<0.05) and 2 h (p<0.05), as compared to the control.Conclusion. These results suggest that ethylmethylhydroxypyridine succinate significantly protects brain mitochondria against microstroke-induced injury. |
| first_indexed | 2024-04-10T02:48:06Z |
| format | Article |
| id | doaj.art-afd7a8158574465da782543a7f6b77ff |
| institution | Directory Open Access Journal |
| issn | 2077-8333 2311-4088 |
| language | Russian |
| last_indexed | 2024-04-10T02:48:06Z |
| publishDate | 2016-08-01 |
| publisher | IRBIS LLC |
| record_format | Article |
| series | Эпилепсия и пароксизмальные состояния |
| spelling | doaj.art-afd7a8158574465da782543a7f6b77ff2023-03-13T07:44:15ZrusIRBIS LLCЭпилепсия и пароксизмальные состояния2077-83332311-40882016-08-0182141910.17749/2077-8333.2016.8.2.014-019227PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPYR. A. Afzalov0Yu. O. Kolikova1E. G. Pryazhnikov2D. V. Toptunov3L. S. Khirug4“Neurotar” Ltd“Neurotar” Ltd“Neurotar” Ltd“Neurotar” Ltd“Neurotar” LtdObjective. Mitochondrial injury plays a central role in neuronal death following ischemic stroke. In the present study, we investigated effects of ethylmethylhydroxypyridine succinate on a microstroke-induced mitochondria swelling, a hallmark of mitochondrial injury.Materials and Methods. Ischemic microstroke was induced in Thy1-CFP-MitoS mice expressing cyano fluorescent protein (CFP) in brain mitochondria by impulse infrared laser. Ethylmethylhydroxypyridine succinate 25 mg/kg or saline (control) were administered i.p. at 30 min after the stroke onset. A period of observation was 48 h. Brain images were obtained by two photon laser fluorescent microscopy and analyzed using a software developed in Neurotar Ltd (Finland). Nonparametric Mann-Whitney-Wilcoxon test was used for te statistical analysis of results.Results. Microstroke resulted in mitochondria swelling, i.e. injury, in the zone surrounding the thrombus. The most profound changes of mitochondrial morphology were observed at 2 h from the stroke onset. Ethylmethylhydroxypyridine succinate significantly reduces the stroke-induced swelling at 1 h (p<0.05) and 2 h (p<0.05), as compared to the control.Conclusion. These results suggest that ethylmethylhydroxypyridine succinate significantly protects brain mitochondria against microstroke-induced injury.https://www.epilepsia.su/jour/article/view/228mitochondriaischemic strokeethylmethylhydroxypyridine succinatefluorescent microscopy |
| spellingShingle | R. A. Afzalov Yu. O. Kolikova E. G. Pryazhnikov D. V. Toptunov L. S. Khirug PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPY Эпилепсия и пароксизмальные состояния mitochondria ischemic stroke ethylmethylhydroxypyridine succinate fluorescent microscopy |
| title | PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPY |
| title_full | PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPY |
| title_fullStr | PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPY |
| title_full_unstemmed | PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPY |
| title_short | PROTECTION OF BRAIN MITOCHONDRIA AGAINST MICRO STROKE-INDUCED INJURY: A STUDY OF ETHYLMETHYLHYDROXYPYRIDINE SUCCINATE IN EXPERIMENTAL MODEL USING TWO PHOTON LASER FLUORESCENT MICROSCOPY |
| title_sort | protection of brain mitochondria against micro stroke induced injury a study of ethylmethylhydroxypyridine succinate in experimental model using two photon laser fluorescent microscopy |
| topic | mitochondria ischemic stroke ethylmethylhydroxypyridine succinate fluorescent microscopy |
| url | https://www.epilepsia.su/jour/article/view/228 |
| work_keys_str_mv | AT raafzalov protectionofbrainmitochondriaagainstmicrostrokeinducedinjuryastudyofethylmethylhydroxypyridinesuccinateinexperimentalmodelusingtwophotonlaserfluorescentmicroscopy AT yuokolikova protectionofbrainmitochondriaagainstmicrostrokeinducedinjuryastudyofethylmethylhydroxypyridinesuccinateinexperimentalmodelusingtwophotonlaserfluorescentmicroscopy AT egpryazhnikov protectionofbrainmitochondriaagainstmicrostrokeinducedinjuryastudyofethylmethylhydroxypyridinesuccinateinexperimentalmodelusingtwophotonlaserfluorescentmicroscopy AT dvtoptunov protectionofbrainmitochondriaagainstmicrostrokeinducedinjuryastudyofethylmethylhydroxypyridinesuccinateinexperimentalmodelusingtwophotonlaserfluorescentmicroscopy AT lskhirug protectionofbrainmitochondriaagainstmicrostrokeinducedinjuryastudyofethylmethylhydroxypyridinesuccinateinexperimentalmodelusingtwophotonlaserfluorescentmicroscopy |