Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites
Recent growing evidence suggests a role for intestinal microbiome and metabolites in patients' postoperative recovery. Therefore, there is a need to gain insight into the impact of pancreaticoduodenectomy for periampullary carcinoma on microbiome and metabolites and the potential impact of thei...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024004249 |
| _version_ | 1827360906053419008 |
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| author | Junwei Fang Chunhong Xiao Yafeng Qi Weixuan Hong Meiping Wang |
| author_facet | Junwei Fang Chunhong Xiao Yafeng Qi Weixuan Hong Meiping Wang |
| author_sort | Junwei Fang |
| collection | DOAJ |
| description | Recent growing evidence suggests a role for intestinal microbiome and metabolites in patients' postoperative recovery. Therefore, there is a need to gain insight into the impact of pancreaticoduodenectomy for periampullary carcinoma on microbiome and metabolites and the potential impact of their changes on patients' condition. Based on 16S rDNA gene sequencing and untargeted metabolomic analysis, we found that the diversity and abundance of intestinal microbiome were significantly higher in patients preoperatively than postoperatively, and the level of intestinal probiotics was significantly lower after surgery compared with preoperatively. In addition, the choline metabolism level was increased and the amino acid metabolism level was decreased after surgery. A total of 53 differential microbiome and 52 differential metabolites were detected, and the differential metabolites were mapped to approximately 60 different KEGG metabolic pathways, of which 13 KEGG metabolic pathways had a differential metabolite number greater than 5. A total of 88 colony-metabolite pairs with significant positive correlation and 69 colony-metabolite pairs with significant negative correlation were identified. Our results reveal alterations in intestinal microbiome after pancreaticoduodenectomy, suggesting its association with postoperative complications. Moreover, the elevated choline metabolism level in postoperative patients may predict their poorer prognosis. At the same time, the decreased abundance of such probiotic bacteria as Prevotella spp. in the postoperative intestine of patients will affect the amino acid metabolism of the organism to some extent. |
| first_indexed | 2024-03-08T06:55:15Z |
| format | Article |
| id | doaj.art-afd8912436b040939ab2ccfe872e94da |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T06:55:15Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-afd8912436b040939ab2ccfe872e94da2024-02-03T06:37:17ZengElsevierHeliyon2405-84402024-01-01102e24393Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolitesJunwei Fang0Chunhong Xiao1Yafeng Qi2Weixuan Hong3Meiping Wang4Department of General Surgery, 900th Hospital of Joint Logistics Support Force of People's Liberation Army, Fuzhou, Fujian, 350000, ChinaDepartment of General Surgery, 900th Hospital of Joint Logistics Support Force of People's Liberation Army, Fuzhou, Fujian, 350000, ChinaDepartment of General Surgery, 900th Hospital of Joint Logistics Support Force of People's Liberation Army, Fuzhou, Fujian, 350000, ChinaDepartment of General Surgery, 900th Hospital of Joint Logistics Support Force of People's Liberation Army, Fuzhou, Fujian, 350000, ChinaCorresponding author.; Department of General Surgery, 900th Hospital of Joint Logistics Support Force of People's Liberation Army, Fuzhou, Fujian, 350000, ChinaRecent growing evidence suggests a role for intestinal microbiome and metabolites in patients' postoperative recovery. Therefore, there is a need to gain insight into the impact of pancreaticoduodenectomy for periampullary carcinoma on microbiome and metabolites and the potential impact of their changes on patients' condition. Based on 16S rDNA gene sequencing and untargeted metabolomic analysis, we found that the diversity and abundance of intestinal microbiome were significantly higher in patients preoperatively than postoperatively, and the level of intestinal probiotics was significantly lower after surgery compared with preoperatively. In addition, the choline metabolism level was increased and the amino acid metabolism level was decreased after surgery. A total of 53 differential microbiome and 52 differential metabolites were detected, and the differential metabolites were mapped to approximately 60 different KEGG metabolic pathways, of which 13 KEGG metabolic pathways had a differential metabolite number greater than 5. A total of 88 colony-metabolite pairs with significant positive correlation and 69 colony-metabolite pairs with significant negative correlation were identified. Our results reveal alterations in intestinal microbiome after pancreaticoduodenectomy, suggesting its association with postoperative complications. Moreover, the elevated choline metabolism level in postoperative patients may predict their poorer prognosis. At the same time, the decreased abundance of such probiotic bacteria as Prevotella spp. in the postoperative intestine of patients will affect the amino acid metabolism of the organism to some extent.http://www.sciencedirect.com/science/article/pii/S2405844024004249PancreaticoduodenectomyIntestinal microbiomeMetabolitesCholinePrevotellaPrognosis |
| spellingShingle | Junwei Fang Chunhong Xiao Yafeng Qi Weixuan Hong Meiping Wang Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites Heliyon Pancreaticoduodenectomy Intestinal microbiome Metabolites Choline Prevotella Prognosis |
| title | Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites |
| title_full | Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites |
| title_fullStr | Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites |
| title_full_unstemmed | Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites |
| title_short | Influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites |
| title_sort | influence of pancreaticoduodenectomy for periampullary carcinoma on intestinal microbiome and metabolites |
| topic | Pancreaticoduodenectomy Intestinal microbiome Metabolites Choline Prevotella Prognosis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024004249 |
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