Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate
To meet<strong> </strong>the growing global demand for gluconic acid as a cement and concrete retarder, inexpensive and abundant lignocellulosic materials are regarded as the most suitable alternatives to starchy materials. However, their enzymatic hydrolysate contains not only glucose b...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/6/562 |
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| author | Lu Cheng Rong Huang Jiaxun Ying Yixiu Fu Xin Zhou Kankan Jiang |
| author_facet | Lu Cheng Rong Huang Jiaxun Ying Yixiu Fu Xin Zhou Kankan Jiang |
| author_sort | Lu Cheng |
| collection | DOAJ |
| description | To meet<strong> </strong>the growing global demand for gluconic acid as a cement and concrete retarder, inexpensive and abundant lignocellulosic materials are regarded as the most suitable alternatives to starchy materials. However, their enzymatic hydrolysate contains not only glucose but also xylose, which negatively affects the performance of gluconic acid as a retarder. Notably, glucose is preferentially bio-oxidized into gluconic acid by <em>Gluconobacter oxydans</em>, but gluconic acid cannot be metabolized by <em>Candida tropicalis</em>. Given this, an artificially designed biological cascade process, respectively employing <em>Gluconobacter oxydans</em> and <em>Candida tropicalis</em>, was established to successfully carry out glucose conversion into gluconic acid, and xylose into a single-cell protein, using the enzymatic hydrolysate of corncobs as a feedstock. This sequential fermentation process produced 95.8 g/L gluconic acid and 9.0 g/L single-cell protein from one liter of the enzymatic hydrolysate that initially contained 98.1 g/L of glucose and 25.4 g/L of xylose. The mass-balance calculation showed that approximately 280 grams of gluconic acid and 27 grams of the single-cell protein could be harvested from 1000 grams of the corncob feedstock. The results suggest that the above-mentioned two-step bioconversion method is efficient in utilizing glucose and xylose from lignocellulosic hydrolysates. |
| first_indexed | 2024-03-11T02:29:58Z |
| format | Article |
| id | doaj.art-afd9bce0999742638cbb70d568891598 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T02:29:58Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-afd9bce0999742638cbb70d5688915982023-11-18T10:21:28ZengMDPI AGFermentation2311-56372023-06-019656210.3390/fermentation9060562Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic HydrolysateLu Cheng0Rong Huang1Jiaxun Ying2Yixiu Fu3Xin Zhou4Kankan Jiang5Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, ChinaSchool of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou 311399, ChinaSchool of Stomatology, Hangzhou Medical College, Hangzhou 311399, ChinaSchool of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou 311399, ChinaJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, ChinaSchool of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou 311399, ChinaTo meet<strong> </strong>the growing global demand for gluconic acid as a cement and concrete retarder, inexpensive and abundant lignocellulosic materials are regarded as the most suitable alternatives to starchy materials. However, their enzymatic hydrolysate contains not only glucose but also xylose, which negatively affects the performance of gluconic acid as a retarder. Notably, glucose is preferentially bio-oxidized into gluconic acid by <em>Gluconobacter oxydans</em>, but gluconic acid cannot be metabolized by <em>Candida tropicalis</em>. Given this, an artificially designed biological cascade process, respectively employing <em>Gluconobacter oxydans</em> and <em>Candida tropicalis</em>, was established to successfully carry out glucose conversion into gluconic acid, and xylose into a single-cell protein, using the enzymatic hydrolysate of corncobs as a feedstock. This sequential fermentation process produced 95.8 g/L gluconic acid and 9.0 g/L single-cell protein from one liter of the enzymatic hydrolysate that initially contained 98.1 g/L of glucose and 25.4 g/L of xylose. The mass-balance calculation showed that approximately 280 grams of gluconic acid and 27 grams of the single-cell protein could be harvested from 1000 grams of the corncob feedstock. The results suggest that the above-mentioned two-step bioconversion method is efficient in utilizing glucose and xylose from lignocellulosic hydrolysates.https://www.mdpi.com/2311-5637/9/6/562gluconic acidenzymatic hydrolysatesingle-cell protein<i>Gluconobacter oxydans</i><i>Candida tropicalis</i> |
| spellingShingle | Lu Cheng Rong Huang Jiaxun Ying Yixiu Fu Xin Zhou Kankan Jiang Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate Fermentation gluconic acid enzymatic hydrolysate single-cell protein <i>Gluconobacter oxydans</i> <i>Candida tropicalis</i> |
| title | Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate |
| title_full | Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate |
| title_fullStr | Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate |
| title_full_unstemmed | Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate |
| title_short | Sequential Bioprocess with <i>Gluconobacter oxydans</i> and <i>Candida tropicalis</i> for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate |
| title_sort | sequential bioprocess with i gluconobacter oxydans i and i candida tropicalis i for gluconic acid and single cell protein production from enzymatic hydrolysate |
| topic | gluconic acid enzymatic hydrolysate single-cell protein <i>Gluconobacter oxydans</i> <i>Candida tropicalis</i> |
| url | https://www.mdpi.com/2311-5637/9/6/562 |
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