Protein Hydration in a Bioprotecting Mixture
We combined broad-band depolarized light scattering and infrared spectroscopies to study the properties of hydration water in a lysozyme-trehalose aqueous solution, where trehalose is present above the concentration threshold (30% in weight) relevant for biopreservation. The joint use of the two dif...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/10/995 |
| _version_ | 1797514124566986752 |
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| author | Silvia Corezzi Brenda Bracco Paola Sassi Marco Paolantoni Lucia Comez |
| author_facet | Silvia Corezzi Brenda Bracco Paola Sassi Marco Paolantoni Lucia Comez |
| author_sort | Silvia Corezzi |
| collection | DOAJ |
| description | We combined broad-band depolarized light scattering and infrared spectroscopies to study the properties of hydration water in a lysozyme-trehalose aqueous solution, where trehalose is present above the concentration threshold (30% in weight) relevant for biopreservation. The joint use of the two different techniques, which were sensitive to inter-and intra-molecular degrees of freedom, shed new light on the molecular mechanism underlying the interaction between the three species in the mixture. Thanks to the comparison with the binary solution cases, we were able to show that, under the investigated conditions, the protein, through preferential hydration, remains strongly hydrated even in the ternary mixture. This supported the water entrapment scenario, for which a certain amount of water between protein and sugar protects the biomolecule from damage caused by external agents. |
| first_indexed | 2024-03-10T06:27:11Z |
| format | Article |
| id | doaj.art-afdb00bd29ed41dfb591f3a74773ac7f |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T06:27:11Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-afdb00bd29ed41dfb591f3a74773ac7f2023-11-22T18:51:50ZengMDPI AGLife2075-17292021-09-01111099510.3390/life11100995Protein Hydration in a Bioprotecting MixtureSilvia Corezzi0Brenda Bracco1Paola Sassi2Marco Paolantoni3Lucia Comez4Dipartimento di Fisica e Geologia, Università degli Studi di Perugia, 06123 Perugia, ItalyDipartimento di Chimica, Biologia e Biotecnologie, Università degli Studi di Perugia, 06123 Perugia, ItalyDipartimento di Chimica, Biologia e Biotecnologie, Università degli Studi di Perugia, 06123 Perugia, ItalyDipartimento di Chimica, Biologia e Biotecnologie, Università degli Studi di Perugia, 06123 Perugia, ItalyCNR-IOM at Dipartimento di Fisica e Geologia, Università degli Studi di Perugia, 06123 Perugia, ItalyWe combined broad-band depolarized light scattering and infrared spectroscopies to study the properties of hydration water in a lysozyme-trehalose aqueous solution, where trehalose is present above the concentration threshold (30% in weight) relevant for biopreservation. The joint use of the two different techniques, which were sensitive to inter-and intra-molecular degrees of freedom, shed new light on the molecular mechanism underlying the interaction between the three species in the mixture. Thanks to the comparison with the binary solution cases, we were able to show that, under the investigated conditions, the protein, through preferential hydration, remains strongly hydrated even in the ternary mixture. This supported the water entrapment scenario, for which a certain amount of water between protein and sugar protects the biomolecule from damage caused by external agents.https://www.mdpi.com/2075-1729/11/10/995hydration waterlight scatteringlysozymetrehalose |
| spellingShingle | Silvia Corezzi Brenda Bracco Paola Sassi Marco Paolantoni Lucia Comez Protein Hydration in a Bioprotecting Mixture Life hydration water light scattering lysozyme trehalose |
| title | Protein Hydration in a Bioprotecting Mixture |
| title_full | Protein Hydration in a Bioprotecting Mixture |
| title_fullStr | Protein Hydration in a Bioprotecting Mixture |
| title_full_unstemmed | Protein Hydration in a Bioprotecting Mixture |
| title_short | Protein Hydration in a Bioprotecting Mixture |
| title_sort | protein hydration in a bioprotecting mixture |
| topic | hydration water light scattering lysozyme trehalose |
| url | https://www.mdpi.com/2075-1729/11/10/995 |
| work_keys_str_mv | AT silviacorezzi proteinhydrationinabioprotectingmixture AT brendabracco proteinhydrationinabioprotectingmixture AT paolasassi proteinhydrationinabioprotectingmixture AT marcopaolantoni proteinhydrationinabioprotectingmixture AT luciacomez proteinhydrationinabioprotectingmixture |