Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis
Objectives: In recent years, commercial molecular tools for diagnosis of invasive aspergillosis have emerged, requiring evaluation to ensure quality. Here we assessed the specificity of Aspergillus spp.-ELITe MGB Assay a commercial assay tergeting 18S gene of Aspergillus spp. Design and methods: As...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Practical Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551724000246 |
| _version_ | 1797291787543379968 |
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| author | Violaine Bothy Maria A. Argudín Nathalie Olive Cindy Barbée Benoît Kabamba-Mukadi Hector Rodriguez-Villalobos |
| author_facet | Violaine Bothy Maria A. Argudín Nathalie Olive Cindy Barbée Benoît Kabamba-Mukadi Hector Rodriguez-Villalobos |
| author_sort | Violaine Bothy |
| collection | DOAJ |
| description | Objectives: In recent years, commercial molecular tools for diagnosis of invasive aspergillosis have emerged, requiring evaluation to ensure quality. Here we assessed the specificity of Aspergillus spp.-ELITe MGB Assay a commercial assay tergeting 18S gene of Aspergillus spp. Design and methods: As part of a method validation, we evaluate the specificity of the Aspergillus spp.-ELITe MGB Assay by testing fourteen culture based samples of sequenced non-Aspergillus fungal species. The benefits of a pre-lysis treatment was evaluated in parallel on serial dilutions of an Aspergillus fumigatus strain. Results: Our findings revealed cross-reactivity in five strains using the 50 copies/mL cut-off recommended by the manufacturer, suggesting potential diagnostic errors and inappropriate management of patients. Pre-lysis treatment does not affect the limit of detection at serial dilution. Conclusions: In conclusion, the Aspergillus spp. ELITe MGB Assay exhibits limited specificity in culture-based samples, underscoring the importance of careful utilization in laboratories. Further studies are warranted to better comprehend of the impact of this cross-reactivity on clinical samples. |
| first_indexed | 2024-03-07T19:41:50Z |
| format | Article |
| id | doaj.art-afdb429aa6884cf985ccced219b9e934 |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-03-07T19:41:50Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-afdb429aa6884cf985ccced219b9e9342024-02-29T05:19:27ZengElsevierPractical Laboratory Medicine2352-55172024-03-0139e00378Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosisViolaine Bothy0Maria A. Argudín1Nathalie Olive2Cindy Barbée3Benoît Kabamba-Mukadi4Hector Rodriguez-Villalobos5Service de microbiologie, Cliniques universitaires Saint-Luc (UCL), Brussels, Belgium; Corresponding author.Service de microbiologie, Cliniques universitaires Saint-Luc (UCL), Brussels, BelgiumService de microbiologie, Cliniques universitaires Saint-Luc (UCL), Brussels, BelgiumService de microbiologie, Cliniques universitaires Saint-Luc (UCL), Brussels, BelgiumService de microbiologie, Cliniques universitaires Saint-Luc (UCL), Brussels, Belgium; Pôle de Recherche en Microbiologie Médicale, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, BelgiumService de microbiologie, Cliniques universitaires Saint-Luc (UCL), Brussels, Belgium; Pôle de Recherche en Microbiologie Médicale, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, BelgiumObjectives: In recent years, commercial molecular tools for diagnosis of invasive aspergillosis have emerged, requiring evaluation to ensure quality. Here we assessed the specificity of Aspergillus spp.-ELITe MGB Assay a commercial assay tergeting 18S gene of Aspergillus spp. Design and methods: As part of a method validation, we evaluate the specificity of the Aspergillus spp.-ELITe MGB Assay by testing fourteen culture based samples of sequenced non-Aspergillus fungal species. The benefits of a pre-lysis treatment was evaluated in parallel on serial dilutions of an Aspergillus fumigatus strain. Results: Our findings revealed cross-reactivity in five strains using the 50 copies/mL cut-off recommended by the manufacturer, suggesting potential diagnostic errors and inappropriate management of patients. Pre-lysis treatment does not affect the limit of detection at serial dilution. Conclusions: In conclusion, the Aspergillus spp. ELITe MGB Assay exhibits limited specificity in culture-based samples, underscoring the importance of careful utilization in laboratories. Further studies are warranted to better comprehend of the impact of this cross-reactivity on clinical samples.http://www.sciencedirect.com/science/article/pii/S2352551724000246AspergillusReal-time PCRElite InGenius |
| spellingShingle | Violaine Bothy Maria A. Argudín Nathalie Olive Cindy Barbée Benoît Kabamba-Mukadi Hector Rodriguez-Villalobos Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis Practical Laboratory Medicine Aspergillus Real-time PCR Elite InGenius |
| title | Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis |
| title_full | Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis |
| title_fullStr | Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis |
| title_full_unstemmed | Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis |
| title_short | Low specificity of Aspergillus spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis |
| title_sort | low specificity of aspergillus spp elite mgb assay and potential risks in management of invasive aspergillosis |
| topic | Aspergillus Real-time PCR Elite InGenius |
| url | http://www.sciencedirect.com/science/article/pii/S2352551724000246 |
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