Dielectric permittivity capable of vitrification cryoprotective media
The research aim was to determine dielectric parameters and state of water in capable of vitrification media PVS2, 88% PVS3 and PVSN, the components of those are glycerol, dimethyl sulfoxide, ethylene glycol and non-penetrating cryoprotectant sucrose. These media are widely used to cryopreserve meri...
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| Format: | Article |
| Language: | English |
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V.N. Karazin Kharkiv National University
2016-10-01
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| Series: | Біофізичний вісник |
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| Online Access: | http://periodicals.karazin.ua/biophysvisnyk/article/view/6499 |
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| author | О. О. Горобченко Н. О. Шевченко О. В. Ліпіна О. Т. Ніколов І. Б. Мусатова |
| author_facet | О. О. Горобченко Н. О. Шевченко О. В. Ліпіна О. Т. Ніколов І. Б. Мусатова |
| author_sort | О. О. Горобченко |
| collection | DOAJ |
| description | The research aim was to determine dielectric parameters and state of water in capable of vitrification media PVS2, 88% PVS3 and PVSN, the components of those are glycerol, dimethyl sulfoxide, ethylene glycol and non-penetrating cryoprotectant sucrose. These media are widely used to cryopreserve meristems, embryoids, callus, allowing the preservation of plant genetic resources under liquid nitrogen conditions for a long time period. Dielectric characteristics of the samples of cryoprotective media, their real ε′ and imaginary ε′′ components were measured at 20 °C using resonant type UHF-dielectrometer at 9.2 GHz frequency. The values of static dielectric permittivity εs of the media on the low-frequency side of the water relaxation and dielectric relaxation frequency of water molecules fd were counted using the Debye equations. It has been shown that the least values of dielectric parameters are characteristic for 88% cryoprotective medium PVS3, which comprises such cryoprotectants as glycerol and sucrose. This medium has the highest ability of binding and ordering the water. When determining the cytotoxicity and effect of low temperature storage on viability of potato, grape, garlic meristems all the studied cryoprotective media can be used when developing the efficient protocols of cryopreservation. |
| first_indexed | 2024-04-13T22:13:02Z |
| format | Article |
| id | doaj.art-afdfc103779b407f85fc7ae0c407b968 |
| institution | Directory Open Access Journal |
| issn | 2075-3810 2075-3829 |
| language | English |
| last_indexed | 2024-04-13T22:13:02Z |
| publishDate | 2016-10-01 |
| publisher | V.N. Karazin Kharkiv National University |
| record_format | Article |
| series | Біофізичний вісник |
| spelling | doaj.art-afdfc103779b407f85fc7ae0c407b9682022-12-22T02:27:39ZengV.N. Karazin Kharkiv National UniversityБіофізичний вісник2075-38102075-38292016-10-0113528336379Dielectric permittivity capable of vitrification cryoprotective mediaО. О. Горобченко0Н. О. Шевченко1О. В. Ліпіна2О. Т. Ніколов3І. Б. Мусатова4Харківський національний університет імені В.Н. КаразінаІнститут проблем кріобіології і кріомедицини НАН УкраїниІнститут проблем кріобіології і кріомедицини НАН УкраїниХарківський національний університет імені В.Н. КаразінаІнститут проблем кріобіології і кріомедицини НАН УкраїниThe research aim was to determine dielectric parameters and state of water in capable of vitrification media PVS2, 88% PVS3 and PVSN, the components of those are glycerol, dimethyl sulfoxide, ethylene glycol and non-penetrating cryoprotectant sucrose. These media are widely used to cryopreserve meristems, embryoids, callus, allowing the preservation of plant genetic resources under liquid nitrogen conditions for a long time period. Dielectric characteristics of the samples of cryoprotective media, their real ε′ and imaginary ε′′ components were measured at 20 °C using resonant type UHF-dielectrometer at 9.2 GHz frequency. The values of static dielectric permittivity εs of the media on the low-frequency side of the water relaxation and dielectric relaxation frequency of water molecules fd were counted using the Debye equations. It has been shown that the least values of dielectric parameters are characteristic for 88% cryoprotective medium PVS3, which comprises such cryoprotectants as glycerol and sucrose. This medium has the highest ability of binding and ordering the water. When determining the cytotoxicity and effect of low temperature storage on viability of potato, grape, garlic meristems all the studied cryoprotective media can be used when developing the efficient protocols of cryopreservation.http://periodicals.karazin.ua/biophysvisnyk/article/view/6499кріозахисні середовищамеристемагліцериндиметилсульфоксидетиленглікольсахарозаНВЧ-діелектрометрія |
| spellingShingle | О. О. Горобченко Н. О. Шевченко О. В. Ліпіна О. Т. Ніколов І. Б. Мусатова Dielectric permittivity capable of vitrification cryoprotective media Біофізичний вісник кріозахисні середовища меристема гліцерин диметилсульфоксид етиленгліколь сахароза НВЧ-діелектрометрія |
| title | Dielectric permittivity capable of vitrification cryoprotective media |
| title_full | Dielectric permittivity capable of vitrification cryoprotective media |
| title_fullStr | Dielectric permittivity capable of vitrification cryoprotective media |
| title_full_unstemmed | Dielectric permittivity capable of vitrification cryoprotective media |
| title_short | Dielectric permittivity capable of vitrification cryoprotective media |
| title_sort | dielectric permittivity capable of vitrification cryoprotective media |
| topic | кріозахисні середовища меристема гліцерин диметилсульфоксид етиленгліколь сахароза НВЧ-діелектрометрія |
| url | http://periodicals.karazin.ua/biophysvisnyk/article/view/6499 |
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