Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes
Background: In ruminants, physiological and nutritional changes occur peripartum. We investigated if gastro-intestinal microbiota, rumen metabolism and antioxidant status were affected around parturition and what could be the impact of a daily supplementation of a live yeast additive in late gestati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/6/447 |
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| author | Lysiane Dunière Damien Esparteiro Yacine Lebbaoui Philippe Ruiz Mickael Bernard Agnès Thomas Denys Durand Evelyne Forano Frédérique Chaucheyras-Durand |
| author_facet | Lysiane Dunière Damien Esparteiro Yacine Lebbaoui Philippe Ruiz Mickael Bernard Agnès Thomas Denys Durand Evelyne Forano Frédérique Chaucheyras-Durand |
| author_sort | Lysiane Dunière |
| collection | DOAJ |
| description | Background: In ruminants, physiological and nutritional changes occur peripartum. We investigated if gastro-intestinal microbiota, rumen metabolism and antioxidant status were affected around parturition and what could be the impact of a daily supplementation of a live yeast additive in late gestating ewes. Methods: Rumen, feces and blood samples were collected from 2 groups of 14 ewes one month and a few days before parturition, and 2 weeks postpartum. Results: In the control ewes close to parturition, slight changes in the ruminal microbiota were observed, with a decrease in the concentration <i>F. succinogenes</i> and in the relative abundance of the Fibrobacteres phylum. Moreover, a decrease in the alpha-diversity of the bacterial community and a reduced relative abundance of the Fibrobacteres phylum were observed in their feces. Control ewes were prone to oxidative stress, as shown by an increase in malondialdehyde (MDA) concentration, a lower total antioxidant status, and higher glutathione peroxidase (GPx) activity in the blood. In the yeast supplemented ewes, most of the microbial changes observed in the control group were alleviated. An increase in GPx activity, and a significant decrease in MDA concentration were measured. Conclusions: The live yeast used in this study could stabilize gastro-intestinal microbiota and reduce oxidative stress close to parturition. |
| first_indexed | 2024-03-10T10:43:13Z |
| format | Article |
| id | doaj.art-afe75833c3a94e3f85a2be8a78dd3b5c |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T10:43:13Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-afe75833c3a94e3f85a2be8a78dd3b5c2023-11-21T22:49:42ZengMDPI AGJournal of Fungi2309-608X2021-06-017644710.3390/jof7060447Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating EwesLysiane Dunière0Damien Esparteiro1Yacine Lebbaoui2Philippe Ruiz3Mickael Bernard4Agnès Thomas5Denys Durand6Evelyne Forano7Frédérique Chaucheyras-Durand8Lallemand SAS, 19 rue des Briquetiers, BP 59, CEDEX, 31702 Blagnac, FranceLallemand SAS, 19 rue des Briquetiers, BP 59, CEDEX, 31702 Blagnac, FranceLallemand SAS, 19 rue des Briquetiers, BP 59, CEDEX, 31702 Blagnac, FranceUMR (Unité Mixte de Recherches) 454 MEDIS (Microbiologie Environnement Digestif et Santé), INRAE, Université Clermont Auvergne, 63122 Saint-Genès Champanelle, FranceUE (Unité Expérimentale) 1414 Herbipôle, INRAE (Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement), Université Clermont Auvergne, 63122 Saint-Genès Champanelle, FranceUMR (Unité Mixte de Recherches) 1213 Herbivores, INRAE (Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement), Université Clermont Auvergne, 63122 Saint-Genès Champanelle, FranceUMR (Unité Mixte de Recherches) 1213 Herbivores, INRAE (Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement), Université Clermont Auvergne, 63122 Saint-Genès Champanelle, FranceUMR (Unité Mixte de Recherches) 454 MEDIS (Microbiologie Environnement Digestif et Santé), INRAE, Université Clermont Auvergne, 63122 Saint-Genès Champanelle, FranceLallemand SAS, 19 rue des Briquetiers, BP 59, CEDEX, 31702 Blagnac, FranceBackground: In ruminants, physiological and nutritional changes occur peripartum. We investigated if gastro-intestinal microbiota, rumen metabolism and antioxidant status were affected around parturition and what could be the impact of a daily supplementation of a live yeast additive in late gestating ewes. Methods: Rumen, feces and blood samples were collected from 2 groups of 14 ewes one month and a few days before parturition, and 2 weeks postpartum. Results: In the control ewes close to parturition, slight changes in the ruminal microbiota were observed, with a decrease in the concentration <i>F. succinogenes</i> and in the relative abundance of the Fibrobacteres phylum. Moreover, a decrease in the alpha-diversity of the bacterial community and a reduced relative abundance of the Fibrobacteres phylum were observed in their feces. Control ewes were prone to oxidative stress, as shown by an increase in malondialdehyde (MDA) concentration, a lower total antioxidant status, and higher glutathione peroxidase (GPx) activity in the blood. In the yeast supplemented ewes, most of the microbial changes observed in the control group were alleviated. An increase in GPx activity, and a significant decrease in MDA concentration were measured. Conclusions: The live yeast used in this study could stabilize gastro-intestinal microbiota and reduce oxidative stress close to parturition.https://www.mdpi.com/2309-608X/7/6/447gestating ewesparturition stressrumen microbiotafecal microbiotalive yeastDNA sequencing |
| spellingShingle | Lysiane Dunière Damien Esparteiro Yacine Lebbaoui Philippe Ruiz Mickael Bernard Agnès Thomas Denys Durand Evelyne Forano Frédérique Chaucheyras-Durand Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes Journal of Fungi gestating ewes parturition stress rumen microbiota fecal microbiota live yeast DNA sequencing |
| title | Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes |
| title_full | Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes |
| title_fullStr | Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes |
| title_full_unstemmed | Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes |
| title_short | Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes |
| title_sort | changes in digestive microbiota rumen fermentations and oxidative stress around parturition are alleviated by live yeast feed supplementation to gestating ewes |
| topic | gestating ewes parturition stress rumen microbiota fecal microbiota live yeast DNA sequencing |
| url | https://www.mdpi.com/2309-608X/7/6/447 |
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