Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration
The regeneration of articular cartilage remains a serious problem in various pathological conditions such as osteoarthritis, due to the tissue’s low self-healing capacity. The latest therapeutic approaches focus on the construction of biomaterials that induce cartilage repair. This research describe...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/7/1890 |
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| author | Aglaia Mantsou Eleni Papachristou Panagiotis Keramidas Paraskevas Lamprou Maria Pitou Rigini M. Papi Katerina Dimitriou Amalia Aggeli Theodora Choli-Papadopoulou |
| author_facet | Aglaia Mantsou Eleni Papachristou Panagiotis Keramidas Paraskevas Lamprou Maria Pitou Rigini M. Papi Katerina Dimitriou Amalia Aggeli Theodora Choli-Papadopoulou |
| author_sort | Aglaia Mantsou |
| collection | DOAJ |
| description | The regeneration of articular cartilage remains a serious problem in various pathological conditions such as osteoarthritis, due to the tissue’s low self-healing capacity. The latest therapeutic approaches focus on the construction of biomaterials that induce cartilage repair. This research describes the design, synthesis, and investigation of a safe, “smart”, fibrous scaffold containing a genetically incorporated active peptide for chondrogenic induction. While possessing specific sequences and the respective mechanical properties from natural fibrous proteins, the fibers also incorporate a Transforming Growth Factor-β1 (TGF-β1)-derived peptide (YYVGRKPK) that can promote chondrogenesis. The scaffold formed stable porous networks with shear-thinning properties at 37 °C, as shown by SEM imaging and rheological characterization, and were proven to be non-toxic to human dental pulp stem cells (hDPSCs). Its chondrogenic capacity was evidenced by a strong increase in the expression of specific chondrogenesis gene markers <i>SOX9</i>, <i>COL2</i>, <i>ACAN</i>, <i>TGFBR1A,</i> and <i>TGFBR2</i> in cells cultured on “scaffold-TGFβ1” for 21 days and by increased phosphorylation of intracellular signaling proteins Smad-2 and Erk-1/2. Additionally, intense staining of glycosaminoglycans was observed in these cells. According to our results, “scaffold-TGFβ1” is proposed for clinical studies as a safe, injectable treatment for cartilage degeneration. |
| first_indexed | 2024-03-11T01:16:55Z |
| format | Article |
| id | doaj.art-afecb07f7f454d119156fa76bda122b5 |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T01:16:55Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-afecb07f7f454d119156fa76bda122b52023-11-18T18:26:27ZengMDPI AGBiomedicines2227-90592023-07-01117189010.3390/biomedicines11071890Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage RegenerationAglaia Mantsou0Eleni Papachristou1Panagiotis Keramidas2Paraskevas Lamprou3Maria Pitou4Rigini M. Papi5Katerina Dimitriou6Amalia Aggeli7Theodora Choli-Papadopoulou8Laboratory of Biochemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Biochemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Biochemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Biochemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Biochemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Biochemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Chemical Engineering A’, School of Chemical Engineering, Faculty of Engineering, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Chemical Engineering A’, School of Chemical Engineering, Faculty of Engineering, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceLaboratory of Biochemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, GreeceThe regeneration of articular cartilage remains a serious problem in various pathological conditions such as osteoarthritis, due to the tissue’s low self-healing capacity. The latest therapeutic approaches focus on the construction of biomaterials that induce cartilage repair. This research describes the design, synthesis, and investigation of a safe, “smart”, fibrous scaffold containing a genetically incorporated active peptide for chondrogenic induction. While possessing specific sequences and the respective mechanical properties from natural fibrous proteins, the fibers also incorporate a Transforming Growth Factor-β1 (TGF-β1)-derived peptide (YYVGRKPK) that can promote chondrogenesis. The scaffold formed stable porous networks with shear-thinning properties at 37 °C, as shown by SEM imaging and rheological characterization, and were proven to be non-toxic to human dental pulp stem cells (hDPSCs). Its chondrogenic capacity was evidenced by a strong increase in the expression of specific chondrogenesis gene markers <i>SOX9</i>, <i>COL2</i>, <i>ACAN</i>, <i>TGFBR1A,</i> and <i>TGFBR2</i> in cells cultured on “scaffold-TGFβ1” for 21 days and by increased phosphorylation of intracellular signaling proteins Smad-2 and Erk-1/2. Additionally, intense staining of glycosaminoglycans was observed in these cells. According to our results, “scaffold-TGFβ1” is proposed for clinical studies as a safe, injectable treatment for cartilage degeneration.https://www.mdpi.com/2227-9059/11/7/1890cartilage regenerationsmart biomaterialschondrogenesistissue engineeringTGF-β1 peptideelastin-like polypeptides |
| spellingShingle | Aglaia Mantsou Eleni Papachristou Panagiotis Keramidas Paraskevas Lamprou Maria Pitou Rigini M. Papi Katerina Dimitriou Amalia Aggeli Theodora Choli-Papadopoulou Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration Biomedicines cartilage regeneration smart biomaterials chondrogenesis tissue engineering TGF-β1 peptide elastin-like polypeptides |
| title | Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration |
| title_full | Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration |
| title_fullStr | Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration |
| title_full_unstemmed | Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration |
| title_short | Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration |
| title_sort | fabrication of a smart fibrous biomaterial that harbors an active tgf β1 peptide a promising approach for cartilage regeneration |
| topic | cartilage regeneration smart biomaterials chondrogenesis tissue engineering TGF-β1 peptide elastin-like polypeptides |
| url | https://www.mdpi.com/2227-9059/11/7/1890 |
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