Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosis
Summary: Lymphedema is a progressive condition accompanying cellulitis and angiosarcoma, suggesting its association with immune dysfunction. Lymphatic venous anastomosis (LVA) can provide relief from cellulitis and angiosarcoma. However, the immune status of peripheral T cells during lymphedema and...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223008994 |
| _version_ | 1797823302717145088 |
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| author | Hirofumi Imai Takakazu Kawase Shuhei Yoshida Toshiro Mese Solji Roh Asuka Fujita Toshio Uchiki Ayano Sasaki Shogo Nagamatsu Atsushi Takazawa Tatsuo Ichinohe Isao Koshima |
| author_facet | Hirofumi Imai Takakazu Kawase Shuhei Yoshida Toshiro Mese Solji Roh Asuka Fujita Toshio Uchiki Ayano Sasaki Shogo Nagamatsu Atsushi Takazawa Tatsuo Ichinohe Isao Koshima |
| author_sort | Hirofumi Imai |
| collection | DOAJ |
| description | Summary: Lymphedema is a progressive condition accompanying cellulitis and angiosarcoma, suggesting its association with immune dysfunction. Lymphatic venous anastomosis (LVA) can provide relief from cellulitis and angiosarcoma. However, the immune status of peripheral T cells during lymphedema and post-LVA remains poorly understood. Using peripheral blood T cells from lymphedema, post-LVA, and healthy controls (HCs), we compared the profile of T cell subsets and T cell receptor (TCR) diversity. PD-1+ Tim-3 + expression was downregulated in post-LVA compared with lymphedema. IFN-γ levels in CD4+PD-1+ T cells and IL-17A levels in CD4+ T cells were downregulated in post-LVA compared with lymphedema. TCR diversity was decreased in lymphedema compared with HCs; such TCR skewing was drastically improved in post-LVA. T cells in lymphedema were associated with exhaustion, inflammation, and diminished diversity, which were relieved post-LVA. The results provide insights into the peripheral T cell population in lymphedema and highlight the immune modulatory importance of LVA. |
| first_indexed | 2024-03-13T10:22:05Z |
| format | Article |
| id | doaj.art-afee97693f3b48eaa3f81187993d6506 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-13T10:22:05Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-afee97693f3b48eaa3f81187993d65062023-05-20T04:30:41ZengElsevieriScience2589-00422023-06-01266106822Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosisHirofumi Imai0Takakazu Kawase1Shuhei Yoshida2Toshiro Mese3Solji Roh4Asuka Fujita5Toshio Uchiki6Ayano Sasaki7Shogo Nagamatsu8Atsushi Takazawa9Tatsuo Ichinohe10Isao Koshima11International Center for Lymphedema, Hiroshima University Hospital, Hiroshima 734-8551, Japan; Corresponding authorDepartment of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan; International Center for Cell and Gene Therapy, Fujita Health University, Toyoake, Aichi 470-1192, JapanInternational Center for Lymphedema, Hiroshima University Hospital, Hiroshima 734-8551, JapanInternational Center for Lymphedema, Hiroshima University Hospital, Hiroshima 734-8551, JapanInternational Center for Lymphedema, Hiroshima University Hospital, Hiroshima 734-8551, JapanDepartment of Plastic and Reconstructive Surgery, Hiroshima University Hospital, Hiroshima 734-8551, JapanDepartment of Plastic and Reconstructive Surgery, Hiroshima University Hospital, Hiroshima 734-8551, JapanDepartment of Plastic and Reconstructive Surgery, Hiroshima University Hospital, Hiroshima 734-8551, JapanDepartment of Plastic and Reconstructive Surgery, Hiroshima University Hospital, Hiroshima 734-8551, JapanDepartment of Orthopaedic Surgery, Hiroshima Hiramatsu Hospital, Hiroshima 732-0816, JapanDepartment of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JapanInternational Center for Lymphedema, Hiroshima University Hospital, Hiroshima 734-8551, Japan; Department of Plastic and Reconstructive Surgery, Hiroshima University Hospital, Hiroshima 734-8551, JapanSummary: Lymphedema is a progressive condition accompanying cellulitis and angiosarcoma, suggesting its association with immune dysfunction. Lymphatic venous anastomosis (LVA) can provide relief from cellulitis and angiosarcoma. However, the immune status of peripheral T cells during lymphedema and post-LVA remains poorly understood. Using peripheral blood T cells from lymphedema, post-LVA, and healthy controls (HCs), we compared the profile of T cell subsets and T cell receptor (TCR) diversity. PD-1+ Tim-3 + expression was downregulated in post-LVA compared with lymphedema. IFN-γ levels in CD4+PD-1+ T cells and IL-17A levels in CD4+ T cells were downregulated in post-LVA compared with lymphedema. TCR diversity was decreased in lymphedema compared with HCs; such TCR skewing was drastically improved in post-LVA. T cells in lymphedema were associated with exhaustion, inflammation, and diminished diversity, which were relieved post-LVA. The results provide insights into the peripheral T cell population in lymphedema and highlight the immune modulatory importance of LVA.http://www.sciencedirect.com/science/article/pii/S2589004223008994Health sciencesImmunologyComponents of the immune system |
| spellingShingle | Hirofumi Imai Takakazu Kawase Shuhei Yoshida Toshiro Mese Solji Roh Asuka Fujita Toshio Uchiki Ayano Sasaki Shogo Nagamatsu Atsushi Takazawa Tatsuo Ichinohe Isao Koshima Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosis iScience Health sciences Immunology Components of the immune system |
| title | Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosis |
| title_full | Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosis |
| title_fullStr | Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosis |
| title_full_unstemmed | Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosis |
| title_short | Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosis |
| title_sort | peripheral t cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post lymphatic venous anastomosis |
| topic | Health sciences Immunology Components of the immune system |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223008994 |
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