Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb.
Sensory deprivation induces dramatic morphological and neurochemical changes in the olfactory bulb (OB) that are largely restricted to glomerular and granule layer interneurons. Mitral cells, pyramidal-like neurons, are resistant to sensory-deprivation-induced changes and are associated with the pre...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3283713?pdf=render |
| _version_ | 1811225002544988160 |
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| author | Thomas Gerald Mast Debra Ann Fadool |
| author_facet | Thomas Gerald Mast Debra Ann Fadool |
| author_sort | Thomas Gerald Mast |
| collection | DOAJ |
| description | Sensory deprivation induces dramatic morphological and neurochemical changes in the olfactory bulb (OB) that are largely restricted to glomerular and granule layer interneurons. Mitral cells, pyramidal-like neurons, are resistant to sensory-deprivation-induced changes and are associated with the precursor to brain-derived neurotrophic factor (proBDNF); here, we investigate its unknown function in the adult mouse OB.As determined using brain-slice electrophysiology in a whole-cell configuration, brain-derived neurotrophic factor (BDNF), but not proBDNF, increased mitral cell excitability. BDNF increased mitral cell action potential firing frequency and decreased interspike interval in response to current injection. In a separate set of experiments, intranasal delivery of neurotrophic factors to awake, adult mice was performed to induce sustained interneuron neurochemical changes. ProBDNF, but not BDNF, increased activated-caspase 3 and reduced tyrosine hydroxylase immunoreactivity in OB glomerular interneurons. In a parallel set of experiments, short-term sensory deprivation produced by unilateral naris occlusion generated an identical phenotype.Our results indicate that only mature BDNF increases mitral cell excitability whereas proBDNF remains ineffective. Our demonstration that proBDNF activates an apoptotic marker in vivo is the first for any proneurotrophin and establishes a role for proBDNF in a model of neuronal plasticity. |
| first_indexed | 2024-04-12T08:59:40Z |
| format | Article |
| id | doaj.art-afeedd85d2d642eeb068653ddd3dd102 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T08:59:40Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-afeedd85d2d642eeb068653ddd3dd1022022-12-22T03:39:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3197810.1371/journal.pone.0031978Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb.Thomas Gerald MastDebra Ann FadoolSensory deprivation induces dramatic morphological and neurochemical changes in the olfactory bulb (OB) that are largely restricted to glomerular and granule layer interneurons. Mitral cells, pyramidal-like neurons, are resistant to sensory-deprivation-induced changes and are associated with the precursor to brain-derived neurotrophic factor (proBDNF); here, we investigate its unknown function in the adult mouse OB.As determined using brain-slice electrophysiology in a whole-cell configuration, brain-derived neurotrophic factor (BDNF), but not proBDNF, increased mitral cell excitability. BDNF increased mitral cell action potential firing frequency and decreased interspike interval in response to current injection. In a separate set of experiments, intranasal delivery of neurotrophic factors to awake, adult mice was performed to induce sustained interneuron neurochemical changes. ProBDNF, but not BDNF, increased activated-caspase 3 and reduced tyrosine hydroxylase immunoreactivity in OB glomerular interneurons. In a parallel set of experiments, short-term sensory deprivation produced by unilateral naris occlusion generated an identical phenotype.Our results indicate that only mature BDNF increases mitral cell excitability whereas proBDNF remains ineffective. Our demonstration that proBDNF activates an apoptotic marker in vivo is the first for any proneurotrophin and establishes a role for proBDNF in a model of neuronal plasticity.http://europepmc.org/articles/PMC3283713?pdf=render |
| spellingShingle | Thomas Gerald Mast Debra Ann Fadool Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb. PLoS ONE |
| title | Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb. |
| title_full | Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb. |
| title_fullStr | Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb. |
| title_full_unstemmed | Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb. |
| title_short | Mature and precursor brain-derived neurotrophic factor have individual roles in the mouse olfactory bulb. |
| title_sort | mature and precursor brain derived neurotrophic factor have individual roles in the mouse olfactory bulb |
| url | http://europepmc.org/articles/PMC3283713?pdf=render |
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