Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. S...
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Format: | Article |
Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2008-03-01
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Series: | Iranian Journal of Chemistry & Chemical Engineering |
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Online Access: | http://www.ijcce.ac.ir/article_7132_d5c60d14f7a56824ba461c2e2ae9756a.pdf |
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author | Mohammad Hosseini Baharak Bahmani Fatollah Moztarzadeh Mohammad Rabiee |
author_facet | Mohammad Hosseini Baharak Bahmani Fatollah Moztarzadeh Mohammad Rabiee |
author_sort | Mohammad Hosseini |
collection | DOAJ |
description | In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated. |
first_indexed | 2024-12-11T21:59:27Z |
format | Article |
id | doaj.art-aff0215f3aa44d1482ae0c7f6b039a4e |
institution | Directory Open Access Journal |
issn | 1021-9986 1021-9986 |
language | English |
last_indexed | 2024-12-11T21:59:27Z |
publishDate | 2008-03-01 |
publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
record_format | Article |
series | Iranian Journal of Chemistry & Chemical Engineering |
spelling | doaj.art-aff0215f3aa44d1482ae0c7f6b039a4e2022-12-22T00:49:11ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862008-03-012711151217132Fabrication of a Sulfite Biosensor by the Use of Conducting PolymerMohammad Hosseini0Baharak Bahmani1Fatollah Moztarzadeh2Mohammad Rabiee3Department of Chemical Engineering, Amirkabir University of Technology, P.O. Box 15874-4413 Tehran, I.R. IRANDepartment of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRANDepartment of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRANDepartment of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRANIn this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated.http://www.ijcce.ac.ir/article_7132_d5c60d14f7a56824ba461c2e2ae9756a.pdfsulfite biosensoranilinesulfite oxidaseconducting polymer |
spellingShingle | Mohammad Hosseini Baharak Bahmani Fatollah Moztarzadeh Mohammad Rabiee Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer Iranian Journal of Chemistry & Chemical Engineering sulfite biosensor aniline sulfite oxidase conducting polymer |
title | Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer |
title_full | Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer |
title_fullStr | Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer |
title_full_unstemmed | Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer |
title_short | Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer |
title_sort | fabrication of a sulfite biosensor by the use of conducting polymer |
topic | sulfite biosensor aniline sulfite oxidase conducting polymer |
url | http://www.ijcce.ac.ir/article_7132_d5c60d14f7a56824ba461c2e2ae9756a.pdf |
work_keys_str_mv | AT mohammadhosseini fabricationofasulfitebiosensorbytheuseofconductingpolymer AT baharakbahmani fabricationofasulfitebiosensorbytheuseofconductingpolymer AT fatollahmoztarzadeh fabricationofasulfitebiosensorbytheuseofconductingpolymer AT mohammadrabiee fabricationofasulfitebiosensorbytheuseofconductingpolymer |