Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer

In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. S...

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Main Authors: Mohammad Hosseini, Baharak Bahmani, Fatollah Moztarzadeh, Mohammad Rabiee
Format: Article
Language:English
Published: Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR 2008-03-01
Series:Iranian Journal of Chemistry & Chemical Engineering
Subjects:
Online Access:http://www.ijcce.ac.ir/article_7132_d5c60d14f7a56824ba461c2e2ae9756a.pdf
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author Mohammad Hosseini
Baharak Bahmani
Fatollah Moztarzadeh
Mohammad Rabiee
author_facet Mohammad Hosseini
Baharak Bahmani
Fatollah Moztarzadeh
Mohammad Rabiee
author_sort Mohammad Hosseini
collection DOAJ
description In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of  the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated.
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spelling doaj.art-aff0215f3aa44d1482ae0c7f6b039a4e2022-12-22T00:49:11ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862008-03-012711151217132Fabrication of a Sulfite Biosensor by the Use of Conducting PolymerMohammad Hosseini0Baharak Bahmani1Fatollah Moztarzadeh2Mohammad Rabiee3Department of Chemical Engineering, Amirkabir University of Technology, P.O. Box 15874-4413 Tehran, I.R. IRANDepartment of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRANDepartment of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRANDepartment of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRANIn this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of  the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated.http://www.ijcce.ac.ir/article_7132_d5c60d14f7a56824ba461c2e2ae9756a.pdfsulfite biosensoranilinesulfite oxidaseconducting polymer
spellingShingle Mohammad Hosseini
Baharak Bahmani
Fatollah Moztarzadeh
Mohammad Rabiee
Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
Iranian Journal of Chemistry & Chemical Engineering
sulfite biosensor
aniline
sulfite oxidase
conducting polymer
title Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
title_full Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
title_fullStr Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
title_full_unstemmed Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
title_short Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
title_sort fabrication of a sulfite biosensor by the use of conducting polymer
topic sulfite biosensor
aniline
sulfite oxidase
conducting polymer
url http://www.ijcce.ac.ir/article_7132_d5c60d14f7a56824ba461c2e2ae9756a.pdf
work_keys_str_mv AT mohammadhosseini fabricationofasulfitebiosensorbytheuseofconductingpolymer
AT baharakbahmani fabricationofasulfitebiosensorbytheuseofconductingpolymer
AT fatollahmoztarzadeh fabricationofasulfitebiosensorbytheuseofconductingpolymer
AT mohammadrabiee fabricationofasulfitebiosensorbytheuseofconductingpolymer