Immunity transfer in mule foals fed with good IgG quality colostrum
While the passive transfer of immunity in horse and donkey foals has been extensively studied, there is limited information for mule foals. Immunoglobulin type G (IgG) and serum total protein concentration (TP) were assessed at different sampling times to evaluate the correlation between serum radia...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024015913 |
| _version_ | 1827348312208965632 |
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| author | Luca Turini Bindi Francesca Vernaccini Matilde Bonelli Francesca Meucci Valentina Sgorbini Micaela |
| author_facet | Luca Turini Bindi Francesca Vernaccini Matilde Bonelli Francesca Meucci Valentina Sgorbini Micaela |
| author_sort | Luca Turini |
| collection | DOAJ |
| description | While the passive transfer of immunity in horse and donkey foals has been extensively studied, there is limited information for mule foals. Immunoglobulin type G (IgG) and serum total protein concentration (TP) were assessed at different sampling times to evaluate the correlation between serum radial immunodiffusion (SRID) with electrophoresis, refractometry, and dry chemistry analyzer (Biuret), and to estimate serum IgG concentrations using serum TP in mule foals. We analyzed a total of 30 samples collected at birth, and at 6, 12, 24, and 48 h of life from 6 mule foals by SRID, electrophoresis TP, biuret TP, and refractometry TP.The SRID IgG concentration significantly increased from birth until T6 (p < 0.001). Serum TP analyzed with refractometry revealed differences between T0 and T12, T24 and T48 (p < 0.05), while a significant difference was observed with the biuret method between T0 and all the other sampling times (p < 0.001).A strong correlation was found between IgG SRID and biuret TP (r = 0.69, p < 0.001), and a good correlation existed between IgG SRID, refractometry TP, and electrophoresis TP (r = 0.44, p < 0.01 and r = 0.39, p < 0.05, respectively).All methods can be used to estimate the passive transfer of immunity in mule foals. TP refractometry and biuret TP values can be used to determine serum IgG concentrations in the blood of mule foals on their first day of life through the application of a specific equation. |
| first_indexed | 2024-03-08T00:10:24Z |
| format | Article |
| id | doaj.art-aff08d7a94324596abdf91cff72e9459 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:10:24Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-aff08d7a94324596abdf91cff72e94592024-02-17T06:41:27ZengElsevierHeliyon2405-84402024-02-01103e25560Immunity transfer in mule foals fed with good IgG quality colostrumLuca Turini0Bindi Francesca1Vernaccini Matilde2Bonelli Francesca3Meucci Valentina4Sgorbini Micaela5Department of Agricultural, Food and Environmental Sciences, University of Pisa, Via Del Borghetto 80, 56124, Pisa, Italy; Corresponding author.Department of Veterinary Sciences, University of Pisa, Viale Delle Piagge 2, 56124, Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale Delle Piagge 2, 56124, Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale Delle Piagge 2, 56124, Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale Delle Piagge 2, 56124, Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale Delle Piagge 2, 56124, Pisa, ItalyWhile the passive transfer of immunity in horse and donkey foals has been extensively studied, there is limited information for mule foals. Immunoglobulin type G (IgG) and serum total protein concentration (TP) were assessed at different sampling times to evaluate the correlation between serum radial immunodiffusion (SRID) with electrophoresis, refractometry, and dry chemistry analyzer (Biuret), and to estimate serum IgG concentrations using serum TP in mule foals. We analyzed a total of 30 samples collected at birth, and at 6, 12, 24, and 48 h of life from 6 mule foals by SRID, electrophoresis TP, biuret TP, and refractometry TP.The SRID IgG concentration significantly increased from birth until T6 (p < 0.001). Serum TP analyzed with refractometry revealed differences between T0 and T12, T24 and T48 (p < 0.05), while a significant difference was observed with the biuret method between T0 and all the other sampling times (p < 0.001).A strong correlation was found between IgG SRID and biuret TP (r = 0.69, p < 0.001), and a good correlation existed between IgG SRID, refractometry TP, and electrophoresis TP (r = 0.44, p < 0.01 and r = 0.39, p < 0.05, respectively).All methods can be used to estimate the passive transfer of immunity in mule foals. TP refractometry and biuret TP values can be used to determine serum IgG concentrations in the blood of mule foals on their first day of life through the application of a specific equation.http://www.sciencedirect.com/science/article/pii/S2405844024015913Mule foalsSerum IgGSerum total proteinRadial immunodiffusionRefractometerElectrophoresis |
| spellingShingle | Luca Turini Bindi Francesca Vernaccini Matilde Bonelli Francesca Meucci Valentina Sgorbini Micaela Immunity transfer in mule foals fed with good IgG quality colostrum Heliyon Mule foals Serum IgG Serum total protein Radial immunodiffusion Refractometer Electrophoresis |
| title | Immunity transfer in mule foals fed with good IgG quality colostrum |
| title_full | Immunity transfer in mule foals fed with good IgG quality colostrum |
| title_fullStr | Immunity transfer in mule foals fed with good IgG quality colostrum |
| title_full_unstemmed | Immunity transfer in mule foals fed with good IgG quality colostrum |
| title_short | Immunity transfer in mule foals fed with good IgG quality colostrum |
| title_sort | immunity transfer in mule foals fed with good igg quality colostrum |
| topic | Mule foals Serum IgG Serum total protein Radial immunodiffusion Refractometer Electrophoresis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024015913 |
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