GREM1 is a potential biomarker for the progression and prognosis of bladder cancer
Abstract Background Gremlin-1 (GREM1) is a protein closely related to tumor growth, although its function in bladder cancer (BCa) is currently unknown. Our first objective was to study the GREM1 treatment potential in BCa. Methods BCa tissue samples were collected for the detection of GREM1 expressi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-08-01
|
| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-023-03128-0 |
| _version_ | 1797452468274069504 |
|---|---|
| author | Peng-cheng Jiang Li-zhe Xu Jin-zhuo Ning Fan Cheng |
| author_facet | Peng-cheng Jiang Li-zhe Xu Jin-zhuo Ning Fan Cheng |
| author_sort | Peng-cheng Jiang |
| collection | DOAJ |
| description | Abstract Background Gremlin-1 (GREM1) is a protein closely related to tumor growth, although its function in bladder cancer (BCa) is currently unknown. Our first objective was to study the GREM1 treatment potential in BCa. Methods BCa tissue samples were collected for the detection of GREM1 expression using Western blot analysis and Immunofluorescence staining. Association of GREM1 expression with clinicopathology and prognosis as detected by TCGA (The Cancer Genome Atlas) database. The functional investigation was tested by qRT-PCR, western blot analysis, CCK-8, cell apoptosis, wound healing, and transwell assays. The interaction between GREM1 and the downstream PI3K/AKT signaling pathway was assessed by Western blot analysis. Results GREM1 exhibited high expression in BCa tissues and was linked to poor prognosis. Stable knockdown of GREM1 significantly inhibited BCa cell (T24 and 5637) proliferation, apoptosis, migratory, invasive, as well as epithelial-mesenchymal transition (EMT) abilities. GREM1 promotes the progression in BCa via PI3K/AKT signaling pathway. Conclusion Findings demonstrate that the progression-promoting effect of GREM1 in BCa, providing a novel biomarker for BCa-targeted therapy. |
| first_indexed | 2024-03-09T15:09:07Z |
| format | Article |
| id | doaj.art-aff2117a741f43798a509c58cb407d50 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-03-09T15:09:07Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-aff2117a741f43798a509c58cb407d502023-11-26T13:31:02ZengBMCWorld Journal of Surgical Oncology1477-78192023-08-0121111110.1186/s12957-023-03128-0GREM1 is a potential biomarker for the progression and prognosis of bladder cancerPeng-cheng Jiang0Li-zhe Xu1Jin-zhuo Ning2Fan Cheng3Department of Urology, Renmin Hospital of Wuhan UniversityDepartment of Urology, Renmin Hospital of Wuhan UniversityDepartment of Urology, Renmin Hospital of Wuhan UniversityDepartment of Urology, Renmin Hospital of Wuhan UniversityAbstract Background Gremlin-1 (GREM1) is a protein closely related to tumor growth, although its function in bladder cancer (BCa) is currently unknown. Our first objective was to study the GREM1 treatment potential in BCa. Methods BCa tissue samples were collected for the detection of GREM1 expression using Western blot analysis and Immunofluorescence staining. Association of GREM1 expression with clinicopathology and prognosis as detected by TCGA (The Cancer Genome Atlas) database. The functional investigation was tested by qRT-PCR, western blot analysis, CCK-8, cell apoptosis, wound healing, and transwell assays. The interaction between GREM1 and the downstream PI3K/AKT signaling pathway was assessed by Western blot analysis. Results GREM1 exhibited high expression in BCa tissues and was linked to poor prognosis. Stable knockdown of GREM1 significantly inhibited BCa cell (T24 and 5637) proliferation, apoptosis, migratory, invasive, as well as epithelial-mesenchymal transition (EMT) abilities. GREM1 promotes the progression in BCa via PI3K/AKT signaling pathway. Conclusion Findings demonstrate that the progression-promoting effect of GREM1 in BCa, providing a novel biomarker for BCa-targeted therapy.https://doi.org/10.1186/s12957-023-03128-0GREM1Bladder cancerProgressionApoptosisPI3K/AKT signaling pathway |
| spellingShingle | Peng-cheng Jiang Li-zhe Xu Jin-zhuo Ning Fan Cheng GREM1 is a potential biomarker for the progression and prognosis of bladder cancer World Journal of Surgical Oncology GREM1 Bladder cancer Progression Apoptosis PI3K/AKT signaling pathway |
| title | GREM1 is a potential biomarker for the progression and prognosis of bladder cancer |
| title_full | GREM1 is a potential biomarker for the progression and prognosis of bladder cancer |
| title_fullStr | GREM1 is a potential biomarker for the progression and prognosis of bladder cancer |
| title_full_unstemmed | GREM1 is a potential biomarker for the progression and prognosis of bladder cancer |
| title_short | GREM1 is a potential biomarker for the progression and prognosis of bladder cancer |
| title_sort | grem1 is a potential biomarker for the progression and prognosis of bladder cancer |
| topic | GREM1 Bladder cancer Progression Apoptosis PI3K/AKT signaling pathway |
| url | https://doi.org/10.1186/s12957-023-03128-0 |
| work_keys_str_mv | AT pengchengjiang grem1isapotentialbiomarkerfortheprogressionandprognosisofbladdercancer AT lizhexu grem1isapotentialbiomarkerfortheprogressionandprognosisofbladdercancer AT jinzhuoning grem1isapotentialbiomarkerfortheprogressionandprognosisofbladdercancer AT fancheng grem1isapotentialbiomarkerfortheprogressionandprognosisofbladdercancer |