Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye
Here we report multiple lines of evidence for a comprehensive model of energy metabolism in the vertebrate eye. Metabolic flux, locations of key enzymes, and our finding that glucose enters mouse and zebrafish retinas mostly through photoreceptors support a conceptually new model for retinal metabol...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/28899 |
| _version_ | 1811200313900662784 |
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| author | Mark A Kanow Michelle M Giarmarco Connor SR Jankowski Kristine Tsantilas Abbi L Engel Jianhai Du Jonathan D Linton Christopher C Farnsworth Stephanie R Sloat Austin Rountree Ian R Sweet Ken J Lindsay Edward D Parker Susan E Brockerhoff Martin Sadilek Jennifer R Chao James B Hurley |
| author_facet | Mark A Kanow Michelle M Giarmarco Connor SR Jankowski Kristine Tsantilas Abbi L Engel Jianhai Du Jonathan D Linton Christopher C Farnsworth Stephanie R Sloat Austin Rountree Ian R Sweet Ken J Lindsay Edward D Parker Susan E Brockerhoff Martin Sadilek Jennifer R Chao James B Hurley |
| author_sort | Mark A Kanow |
| collection | DOAJ |
| description | Here we report multiple lines of evidence for a comprehensive model of energy metabolism in the vertebrate eye. Metabolic flux, locations of key enzymes, and our finding that glucose enters mouse and zebrafish retinas mostly through photoreceptors support a conceptually new model for retinal metabolism. In this model, glucose from the choroidal blood passes through the retinal pigment epithelium to the retina where photoreceptors convert it to lactate. Photoreceptors then export the lactate as fuel for the retinal pigment epithelium and for neighboring Müller glial cells. We used human retinal epithelial cells to show that lactate can suppress consumption of glucose by the retinal pigment epithelium. Suppression of glucose consumption in the retinal pigment epithelium can increase the amount of glucose that reaches the retina. This framework for understanding metabolic relationships in the vertebrate retina provides new insights into the underlying causes of retinal disease and age-related vision loss. |
| first_indexed | 2024-04-12T02:01:36Z |
| format | Article |
| id | doaj.art-aff27e94dd9b414d9153404c6b6c9551 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:01:36Z |
| publishDate | 2017-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-aff27e94dd9b414d9153404c6b6c95512022-12-22T03:52:39ZengeLife Sciences Publications LtdeLife2050-084X2017-09-01610.7554/eLife.28899Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eyeMark A Kanow0Michelle M Giarmarco1https://orcid.org/0000-0003-3344-4268Connor SR Jankowski2Kristine Tsantilas3Abbi L Engel4Jianhai Du5Jonathan D Linton6Christopher C Farnsworth7Stephanie R Sloat8Austin Rountree9Ian R Sweet10Ken J Lindsay11Edward D Parker12Susan E Brockerhoff13Martin Sadilek14Jennifer R Chao15https://orcid.org/0000-0002-6859-5552James B Hurley16https://orcid.org/0000-0002-7754-0705Department of Biochemistry, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United StatesDepartment of Ophthalmology, University of Washington, Seattle, United StatesDepartment of Ophthalmology, West Virginia University, Morgantown, United States; Department of Biochemistry, West Virginia University, Morgantown, United StatesDepartment of Biochemistry, University of Washington, Seattle, United States; Department of Ophthalmology, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United StatesDepartment of Medicine, UW Diabetes Institute, University of Washington, Seattle, United StatesDepartment of Medicine, UW Diabetes Institute, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United States; Fred Hutchinson Cancer Research Center, Seattle, United StatesDepartment of Ophthalmology, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United States; Department of Ophthalmology, University of Washington, Seattle, United StatesDepartment of Chemistry, University of Washington, Seattle, United StatesDepartment of Ophthalmology, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United States; Department of Ophthalmology, University of Washington, Seattle, United StatesHere we report multiple lines of evidence for a comprehensive model of energy metabolism in the vertebrate eye. Metabolic flux, locations of key enzymes, and our finding that glucose enters mouse and zebrafish retinas mostly through photoreceptors support a conceptually new model for retinal metabolism. In this model, glucose from the choroidal blood passes through the retinal pigment epithelium to the retina where photoreceptors convert it to lactate. Photoreceptors then export the lactate as fuel for the retinal pigment epithelium and for neighboring Müller glial cells. We used human retinal epithelial cells to show that lactate can suppress consumption of glucose by the retinal pigment epithelium. Suppression of glucose consumption in the retinal pigment epithelium can increase the amount of glucose that reaches the retina. This framework for understanding metabolic relationships in the vertebrate retina provides new insights into the underlying causes of retinal disease and age-related vision loss.https://elifesciences.org/articles/28899retinaenergy metabolismphotoreceptors |
| spellingShingle | Mark A Kanow Michelle M Giarmarco Connor SR Jankowski Kristine Tsantilas Abbi L Engel Jianhai Du Jonathan D Linton Christopher C Farnsworth Stephanie R Sloat Austin Rountree Ian R Sweet Ken J Lindsay Edward D Parker Susan E Brockerhoff Martin Sadilek Jennifer R Chao James B Hurley Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye eLife retina energy metabolism photoreceptors |
| title | Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye |
| title_full | Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye |
| title_fullStr | Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye |
| title_full_unstemmed | Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye |
| title_short | Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye |
| title_sort | biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye |
| topic | retina energy metabolism photoreceptors |
| url | https://elifesciences.org/articles/28899 |
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