Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species

Metallothioneins play a key role in maintaining homeostasis of essential metalsand in protecting of cells against metal toxicity as well as oxidative damaging. Exceptinghumans, blood levels of metallothionein have not yet been reported from any animalspecies. Blood plasma samples of 9 animal species...

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Main Authors: Rene Kizek, Libuse Trnkova, Jaromir Hubalek, Jiri Pikula, Miroslava Beklova, Vojtech Adam
Format: Article
Language:English
Published: MDPI AG 2007-10-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/7/10/2419/
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author Rene Kizek
Libuse Trnkova
Jaromir Hubalek
Jiri Pikula
Miroslava Beklova
Vojtech Adam
author_facet Rene Kizek
Libuse Trnkova
Jaromir Hubalek
Jiri Pikula
Miroslava Beklova
Vojtech Adam
author_sort Rene Kizek
collection DOAJ
description Metallothioneins play a key role in maintaining homeostasis of essential metalsand in protecting of cells against metal toxicity as well as oxidative damaging. Exceptinghumans, blood levels of metallothionein have not yet been reported from any animalspecies. Blood plasma samples of 9 animal species were analysed by the adsorptive transferstripping technique to obtain species specific voltammograms. Quite distinct records wereobtained from the Takin (Budorcas taxicolor), while other interesting records were observedin samples from the European Bison (Bison bonasus bonasus) and the Red-eared Slider(Trachemys scripta elegans). To quantify metallothionein the catalytic peak Cat2 was used,well developed in the Domestic Fowl (Gallus gallus f. domestica) and showing a very lowsignal in the Red Deer (Cervus elaphus). The highest levels of metallothionein reachingover 20 μM were found in the Domestic Fowl. High levels of MT were also found in theBearded Dragon (Pogona vitticeps) and the Grey Wolf (Canis lupus lupus). The lowestvalues of about 1-3 μM were determined in the Red-eared Slider, Takin and Red Deer. Employing a simple electrochemical detection it was possible to examine variation in blood metallothionein in different species of vertebrates.
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spelling doaj.art-aff2b34b20474063b44ecfa0eb10ab402022-12-22T04:25:19ZengMDPI AGSensors1424-82202007-10-017102419242910.3390/s7102419Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal SpeciesRene KizekLibuse TrnkovaJaromir HubalekJiri PikulaMiroslava BeklovaVojtech AdamMetallothioneins play a key role in maintaining homeostasis of essential metalsand in protecting of cells against metal toxicity as well as oxidative damaging. Exceptinghumans, blood levels of metallothionein have not yet been reported from any animalspecies. Blood plasma samples of 9 animal species were analysed by the adsorptive transferstripping technique to obtain species specific voltammograms. Quite distinct records wereobtained from the Takin (Budorcas taxicolor), while other interesting records were observedin samples from the European Bison (Bison bonasus bonasus) and the Red-eared Slider(Trachemys scripta elegans). To quantify metallothionein the catalytic peak Cat2 was used,well developed in the Domestic Fowl (Gallus gallus f. domestica) and showing a very lowsignal in the Red Deer (Cervus elaphus). The highest levels of metallothionein reachingover 20 μM were found in the Domestic Fowl. High levels of MT were also found in theBearded Dragon (Pogona vitticeps) and the Grey Wolf (Canis lupus lupus). The lowestvalues of about 1-3 μM were determined in the Red-eared Slider, Takin and Red Deer. Employing a simple electrochemical detection it was possible to examine variation in blood metallothionein in different species of vertebrates.http://www.mdpi.com/1424-8220/7/10/2419/Electrochemical detectionCatalytic signalBrdicka reactionHeavy metalsRed-eared SliderBearded DragonDomestic FowlGrey WolfBactrian CamelReindeerTakinEuropean BisonRed Deer
spellingShingle Rene Kizek
Libuse Trnkova
Jaromir Hubalek
Jiri Pikula
Miroslava Beklova
Vojtech Adam
Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
Sensors
Electrochemical detection
Catalytic signal
Brdicka reaction
Heavy metals
Red-eared Slider
Bearded Dragon
Domestic Fowl
Grey Wolf
Bactrian Camel
Reindeer
Takin
European Bison
Red Deer
title Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
title_full Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
title_fullStr Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
title_full_unstemmed Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
title_short Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
title_sort shapes of differential pulse voltammograms and level of metallothionein at different animal species
topic Electrochemical detection
Catalytic signal
Brdicka reaction
Heavy metals
Red-eared Slider
Bearded Dragon
Domestic Fowl
Grey Wolf
Bactrian Camel
Reindeer
Takin
European Bison
Red Deer
url http://www.mdpi.com/1424-8220/7/10/2419/
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