Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
Metallothioneins play a key role in maintaining homeostasis of essential metalsand in protecting of cells against metal toxicity as well as oxidative damaging. Exceptinghumans, blood levels of metallothionein have not yet been reported from any animalspecies. Blood plasma samples of 9 animal species...
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MDPI AG
2007-10-01
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author | Rene Kizek Libuse Trnkova Jaromir Hubalek Jiri Pikula Miroslava Beklova Vojtech Adam |
author_facet | Rene Kizek Libuse Trnkova Jaromir Hubalek Jiri Pikula Miroslava Beklova Vojtech Adam |
author_sort | Rene Kizek |
collection | DOAJ |
description | Metallothioneins play a key role in maintaining homeostasis of essential metalsand in protecting of cells against metal toxicity as well as oxidative damaging. Exceptinghumans, blood levels of metallothionein have not yet been reported from any animalspecies. Blood plasma samples of 9 animal species were analysed by the adsorptive transferstripping technique to obtain species specific voltammograms. Quite distinct records wereobtained from the Takin (Budorcas taxicolor), while other interesting records were observedin samples from the European Bison (Bison bonasus bonasus) and the Red-eared Slider(Trachemys scripta elegans). To quantify metallothionein the catalytic peak Cat2 was used,well developed in the Domestic Fowl (Gallus gallus f. domestica) and showing a very lowsignal in the Red Deer (Cervus elaphus). The highest levels of metallothionein reachingover 20 μM were found in the Domestic Fowl. High levels of MT were also found in theBearded Dragon (Pogona vitticeps) and the Grey Wolf (Canis lupus lupus). The lowestvalues of about 1-3 μM were determined in the Red-eared Slider, Takin and Red Deer. Employing a simple electrochemical detection it was possible to examine variation in blood metallothionein in different species of vertebrates. |
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issn | 1424-8220 |
language | English |
last_indexed | 2024-04-11T11:51:17Z |
publishDate | 2007-10-01 |
publisher | MDPI AG |
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spelling | doaj.art-aff2b34b20474063b44ecfa0eb10ab402022-12-22T04:25:19ZengMDPI AGSensors1424-82202007-10-017102419242910.3390/s7102419Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal SpeciesRene KizekLibuse TrnkovaJaromir HubalekJiri PikulaMiroslava BeklovaVojtech AdamMetallothioneins play a key role in maintaining homeostasis of essential metalsand in protecting of cells against metal toxicity as well as oxidative damaging. Exceptinghumans, blood levels of metallothionein have not yet been reported from any animalspecies. Blood plasma samples of 9 animal species were analysed by the adsorptive transferstripping technique to obtain species specific voltammograms. Quite distinct records wereobtained from the Takin (Budorcas taxicolor), while other interesting records were observedin samples from the European Bison (Bison bonasus bonasus) and the Red-eared Slider(Trachemys scripta elegans). To quantify metallothionein the catalytic peak Cat2 was used,well developed in the Domestic Fowl (Gallus gallus f. domestica) and showing a very lowsignal in the Red Deer (Cervus elaphus). The highest levels of metallothionein reachingover 20 μM were found in the Domestic Fowl. High levels of MT were also found in theBearded Dragon (Pogona vitticeps) and the Grey Wolf (Canis lupus lupus). The lowestvalues of about 1-3 μM were determined in the Red-eared Slider, Takin and Red Deer. Employing a simple electrochemical detection it was possible to examine variation in blood metallothionein in different species of vertebrates.http://www.mdpi.com/1424-8220/7/10/2419/Electrochemical detectionCatalytic signalBrdicka reactionHeavy metalsRed-eared SliderBearded DragonDomestic FowlGrey WolfBactrian CamelReindeerTakinEuropean BisonRed Deer |
spellingShingle | Rene Kizek Libuse Trnkova Jaromir Hubalek Jiri Pikula Miroslava Beklova Vojtech Adam Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species Sensors Electrochemical detection Catalytic signal Brdicka reaction Heavy metals Red-eared Slider Bearded Dragon Domestic Fowl Grey Wolf Bactrian Camel Reindeer Takin European Bison Red Deer |
title | Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species |
title_full | Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species |
title_fullStr | Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species |
title_full_unstemmed | Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species |
title_short | Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species |
title_sort | shapes of differential pulse voltammograms and level of metallothionein at different animal species |
topic | Electrochemical detection Catalytic signal Brdicka reaction Heavy metals Red-eared Slider Bearded Dragon Domestic Fowl Grey Wolf Bactrian Camel Reindeer Takin European Bison Red Deer |
url | http://www.mdpi.com/1424-8220/7/10/2419/ |
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