Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder?
Background and aims: Portal vein thrombosis (PVT) is a critical complication in cirrhotic patients. We explored the role of the activated factor VII-antithrombin (FVIIa-AT) complex and enhanced monocytic tissue factor (TF) expression in the development and prediction of non-neoplastic PVT in cirrhot...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119310956 |
| _version_ | 1818602774748725248 |
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| author | Mona A. Abu El-Makarem Atef F. El-Akad Moustafa M. Elian Tahra M. Sherif Ragaa A. El-shaheed Aliaa S. Abd EL Fatah Douaa M. Sayed Rania M. Bakry Aisha M. Mahmoud |
| author_facet | Mona A. Abu El-Makarem Atef F. El-Akad Moustafa M. Elian Tahra M. Sherif Ragaa A. El-shaheed Aliaa S. Abd EL Fatah Douaa M. Sayed Rania M. Bakry Aisha M. Mahmoud |
| author_sort | Mona A. Abu El-Makarem |
| collection | DOAJ |
| description | Background and aims: Portal vein thrombosis (PVT) is a critical complication in cirrhotic patients. We explored the role of the activated factor VII-antithrombin (FVIIa-AT) complex and enhanced monocytic tissue factor (TF) expression in the development and prediction of non-neoplastic PVT in cirrhotic patients. Material and methods: A total of 30 HCV-cirrhosis patients were included in our study. They were compared to 35 cirrhotic patients without PVT, 15 non-cirrhotic patients with PVT, and 15 healthy controls. The plasma level of the FVIIa-AT complexes was analyzed by ELISA. MIF CD142, CD86, and HLA-DR on monocytes (CD14) were determined by flow cytometry. Results: Compared with cirrhotic patients without PVT, cirrhotic patients with PVT had comparable plasma values of FVIIa, AT, and the FVIIa-AT complex. However, they had significantly lower values compared to non-cirrhotic patients with PVT and healthy controls. Cirrhotic patients with PVT had increased monocytic TF expression (MIF CD142) compared to non-PVT cirrhotic patients and healthy controls [86.5 (93.5) vs. 18 (32.0) and 11.0 (6.0), respectively; p < 0.001 for each]. However, cirrhosis PVT could not be distinguished from non-cirrhosis PVT. The area under the ROC curve of MIF CD142 was 0.759 (0.641-0.876; p = 0.000) at an optimal cut-off value of 45, which yielded a sensitivity of 60% and a specificity of 77.1%, as well as a PPV and NPV of 69.2% for each. Conclusion: Enhanced expression of monocytic TF may have a role in the development and prediction of non-neoplastic PVT in HCV-cirrhosis patients. Large multicenter studies are necessary to validate our results. |
| first_indexed | 2024-12-16T13:12:38Z |
| format | Article |
| id | doaj.art-aff5e25fd47340098188e53e854ddcad |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-16T13:12:38Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-aff5e25fd47340098188e53e854ddcad2022-12-21T22:30:34ZengElsevierAnnals of Hepatology1665-26812017-07-01164574583Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder?Mona A. Abu El-Makarem0Atef F. El-Akad1Moustafa M. Elian2Tahra M. Sherif3Ragaa A. El-shaheed4Aliaa S. Abd EL Fatah5Douaa M. Sayed6Rania M. Bakry7Aisha M. Mahmoud8Department of Internal Medicine, School of Medicine, Minia University, Minia, Egypt; Correspondence and reprint request:Department of Internal Medicine, School of Medicine, Minia University, Minia, EgyptDepartment of Radio-Diagnosis, School of Medicine, Minia University, Minia, EgyptDepartment of Clinical Pathology, School of Medicine, Minia University, Minia, EgyptDepartment of Internal Medicine, School of Medicine, Minia University, Minia, EgyptDepartment of Internal Medicine, School of Medicine, Minia University, Minia, EgyptDepartment of Clinical Pathology, South Egypt Cancer Institute, Assuit University, Assuit, EgyptDepartment of Clinical Pathology, South Egypt Cancer Institute, Assuit University, Assuit, EgyptDepartment of Internal Medicine, School of Medicine, Minia University, Minia, EgyptBackground and aims: Portal vein thrombosis (PVT) is a critical complication in cirrhotic patients. We explored the role of the activated factor VII-antithrombin (FVIIa-AT) complex and enhanced monocytic tissue factor (TF) expression in the development and prediction of non-neoplastic PVT in cirrhotic patients. Material and methods: A total of 30 HCV-cirrhosis patients were included in our study. They were compared to 35 cirrhotic patients without PVT, 15 non-cirrhotic patients with PVT, and 15 healthy controls. The plasma level of the FVIIa-AT complexes was analyzed by ELISA. MIF CD142, CD86, and HLA-DR on monocytes (CD14) were determined by flow cytometry. Results: Compared with cirrhotic patients without PVT, cirrhotic patients with PVT had comparable plasma values of FVIIa, AT, and the FVIIa-AT complex. However, they had significantly lower values compared to non-cirrhotic patients with PVT and healthy controls. Cirrhotic patients with PVT had increased monocytic TF expression (MIF CD142) compared to non-PVT cirrhotic patients and healthy controls [86.5 (93.5) vs. 18 (32.0) and 11.0 (6.0), respectively; p < 0.001 for each]. However, cirrhosis PVT could not be distinguished from non-cirrhosis PVT. The area under the ROC curve of MIF CD142 was 0.759 (0.641-0.876; p = 0.000) at an optimal cut-off value of 45, which yielded a sensitivity of 60% and a specificity of 77.1%, as well as a PPV and NPV of 69.2% for each. Conclusion: Enhanced expression of monocytic TF may have a role in the development and prediction of non-neoplastic PVT in HCV-cirrhosis patients. Large multicenter studies are necessary to validate our results.http://www.sciencedirect.com/science/article/pii/S1665268119310956Portal hypertensionTissue factorFlow cytometry |
| spellingShingle | Mona A. Abu El-Makarem Atef F. El-Akad Moustafa M. Elian Tahra M. Sherif Ragaa A. El-shaheed Aliaa S. Abd EL Fatah Douaa M. Sayed Rania M. Bakry Aisha M. Mahmoud Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder? Annals of Hepatology Portal hypertension Tissue factor Flow cytometry |
| title | Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder? |
| title_full | Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder? |
| title_fullStr | Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder? |
| title_full_unstemmed | Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder? |
| title_short | Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: is it an Immuno-Inflammatory Disorder? |
| title_sort | non neoplastic portal vein thrombosis in hcv cirrhosis patients is it an immuno inflammatory disorder |
| topic | Portal hypertension Tissue factor Flow cytometry |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119310956 |
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