Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs
AIM: To investigate the effects of green flickering light on refractive development and expression of muscarinic acetylcholine receptor (mAChR) M1 in the eyes of guinea pigs. METHODS: Thirty guinea pigs (15-20 days old) were randomly divided into three groups (n=10/group). Animals in group I were r...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-11-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2018/11/20181104.pdf |
| _version_ | 1818571650433548288 |
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| author | Yuan Tao Xiao-Li Li Li-Yuan Sun Yu-Hua Wei Xiao-Ting Yu Hong Wang |
| author_facet | Yuan Tao Xiao-Li Li Li-Yuan Sun Yu-Hua Wei Xiao-Ting Yu Hong Wang |
| author_sort | Yuan Tao |
| collection | DOAJ |
| description | AIM: To investigate the effects of green flickering light on refractive development and expression of muscarinic acetylcholine receptor (mAChR) M1 in the eyes of guinea pigs.
METHODS: Thirty guinea pigs (15-20 days old) were randomly divided into three groups (n=10/group). Animals in group I were raised in a completely closed carton with green flickering light illumination. Those in group II were kept in the open top closed carton under normal natural light. Guinea pigs were raised in a sight-widen cage under normal natural light in group III. The refractive status and axial length were measured before and after 8 weeks' illumination. Moreover, total RNA extracted from retinal, choroidal, and scleral tissues were determined by real-time reverse transcription polymerase chain reaction (RT-PCR). The expressions of the receptor M1 were also explored in the retina, choroid, and sclera using immunohistochemistry.
RESULTS: There was a remarkable reduction in refractive error and increase in axial length after 8-weeks' green flickering light stimulation (P<0.001). The expression of M1 receptor mRNA in sclera and retina in myopia group were remarkably lower than that in group II and III (P<0.01). Significant reduced expression of M1 receptor stimulated by green flickering light in retina and sclera tissues were also observed (P<0.05). However, there was no M1 receptor expression in choroid in 3 groups.
CONCLUSION: Myopia can be induced by 8 weeks' green flickering light exposure in the animal model. M1 receptor may be involved causally or protectively in myopia development. |
| first_indexed | 2024-12-14T13:58:50Z |
| format | Article |
| id | doaj.art-aff6f2bd9393467fb756a2cdf46554fd |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-14T13:58:50Z |
| publishDate | 2018-11-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-aff6f2bd9393467fb756a2cdf46554fd2022-12-21T22:58:46ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-11-0111111755176010.18240/ijo.2018.11.04Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigsYuan Tao0Xiao-Li Li1Li-Yuan Sun2Yu-Hua Wei3Xiao-Ting Yu4Hong Wang5Department of Ophthalmology, the Second People’s Hospital of Jinan City, Jinan 250001, Shandong Province, ChinaDepartment of Laboratory Medicine, Qilu Hospital of Shandong University, Jinan 250001, Shandong Province, ChinaDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250001, Shandong Province, ChinaDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250001, Shandong Province, ChinaDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250001, Shandong Province, ChinaDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250001, Shandong Province, ChinaAIM: To investigate the effects of green flickering light on refractive development and expression of muscarinic acetylcholine receptor (mAChR) M1 in the eyes of guinea pigs. METHODS: Thirty guinea pigs (15-20 days old) were randomly divided into three groups (n=10/group). Animals in group I were raised in a completely closed carton with green flickering light illumination. Those in group II were kept in the open top closed carton under normal natural light. Guinea pigs were raised in a sight-widen cage under normal natural light in group III. The refractive status and axial length were measured before and after 8 weeks' illumination. Moreover, total RNA extracted from retinal, choroidal, and scleral tissues were determined by real-time reverse transcription polymerase chain reaction (RT-PCR). The expressions of the receptor M1 were also explored in the retina, choroid, and sclera using immunohistochemistry. RESULTS: There was a remarkable reduction in refractive error and increase in axial length after 8-weeks' green flickering light stimulation (P<0.001). The expression of M1 receptor mRNA in sclera and retina in myopia group were remarkably lower than that in group II and III (P<0.01). Significant reduced expression of M1 receptor stimulated by green flickering light in retina and sclera tissues were also observed (P<0.05). However, there was no M1 receptor expression in choroid in 3 groups. CONCLUSION: Myopia can be induced by 8 weeks' green flickering light exposure in the animal model. M1 receptor may be involved causally or protectively in myopia development.http://www.ijo.cn/en_publish/2018/11/20181104.pdfguinea pigsgreen flashing lightmyopia modelmuscarinic acetylcholine 1 receptor |
| spellingShingle | Yuan Tao Xiao-Li Li Li-Yuan Sun Yu-Hua Wei Xiao-Ting Yu Hong Wang Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs International Journal of Ophthalmology guinea pigs green flashing light myopia model muscarinic acetylcholine 1 receptor |
| title | Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs |
| title_full | Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs |
| title_fullStr | Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs |
| title_full_unstemmed | Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs |
| title_short | Effect of green flickering light on myopia development and expression of M1 muscarinic acetylcholine receptor in guinea pigs |
| title_sort | effect of green flickering light on myopia development and expression of m1 muscarinic acetylcholine receptor in guinea pigs |
| topic | guinea pigs green flashing light myopia model muscarinic acetylcholine 1 receptor |
| url | http://www.ijo.cn/en_publish/2018/11/20181104.pdf |
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