Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia
Wondwossen Tsegaye,1 Adane Bitew,2 Addisu Gize3 1Department of Diagnostic Medical Laboratory and Radiology, Defense University, Bishoftu, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Microbiology, St....
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-04-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/bacterial-contamination-and-susceptibility-pattern-among-blood-and-blo-peer-reviewed-fulltext-article-IDR |
| _version_ | 1811313253747261440 |
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| author | Tsegaye W Bitew A Gize A |
| author_facet | Tsegaye W Bitew A Gize A |
| author_sort | Tsegaye W |
| collection | DOAJ |
| description | Wondwossen Tsegaye,1 Adane Bitew,2 Addisu Gize3 1Department of Diagnostic Medical Laboratory and Radiology, Defense University, Bishoftu, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Microbiology, St. Paul’s Hospital Millennium Medical College, Addis Ababa, EthiopiaCorrespondence: Addisu Gize, Email addisu.gize@sphmmc.edu.etBackground: Understanding the proportion of blood and blood products contamination during transfusion is important in developing infection control strategy in the health system. Therefore, this study was aimed to determine bacterial contamination and susceptibility patterns among blood and blood components at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia.Methods: A cross-sectional study was done on blood and blood components collected with both diverging and none-diverging methods. Simple random sampling method was applied to select blood and blood product units from the record form and 10 mL of sample was drawn from each blood and blood product. Culturing from collected blood and blood products and antimicrobial sensitivity tests were done. The collected data were entered and analyzed by SPSS statistical software version 20. Binary and multiple logistic regression analysis were done to see different factors and p-values less than 0.05 were taken as statistically significant.Results: A total of three hundred seventy six (376) units of blood and blood product samples, 188 from non-diverging and 188 from diverging blood donor collection methods were included. Among different blood group types, O+ blood and the majority of components were stored from one to five days. The overall bacterial contamination among blood and blood components were n = 17 (4.5%). Among them, n = 14 (7.4%) and n = 3 (1.6%) were collected with a non-diverging and diverging method, respectively. Staphylococcus epidermidis was the most dominant isolate. Most bacterial isolates were susceptible to different antimicrobial agents; however, Pseudomonas aeruginosa showed resistance for gentamicin. The non-diverging collection units of blood and components were significantly associated with bacterial contamination. Of all, n = 5/17 (29.4%) isolated bacteria were multidrug resistant.Conclusion: The bacterial contamination of blood and blood components collected with diverging method was lower and recommended to use this collection method from donors.Keywords: bacterial contamination, diverging and non-diverging method, blood culture, susceptibility pattern |
| first_indexed | 2024-04-13T10:50:27Z |
| format | Article |
| id | doaj.art-affa6d89fecf40489a77168de751ab1e |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-13T10:50:27Z |
| publishDate | 2022-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-affa6d89fecf40489a77168de751ab1e2022-12-22T02:49:41ZengDove Medical PressInfection and Drug Resistance1178-69732022-04-01Volume 151677168674317Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, EthiopiaTsegaye WBitew AGize AWondwossen Tsegaye,1 Adane Bitew,2 Addisu Gize3 1Department of Diagnostic Medical Laboratory and Radiology, Defense University, Bishoftu, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Microbiology, St. Paul’s Hospital Millennium Medical College, Addis Ababa, EthiopiaCorrespondence: Addisu Gize, Email addisu.gize@sphmmc.edu.etBackground: Understanding the proportion of blood and blood products contamination during transfusion is important in developing infection control strategy in the health system. Therefore, this study was aimed to determine bacterial contamination and susceptibility patterns among blood and blood components at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia.Methods: A cross-sectional study was done on blood and blood components collected with both diverging and none-diverging methods. Simple random sampling method was applied to select blood and blood product units from the record form and 10 mL of sample was drawn from each blood and blood product. Culturing from collected blood and blood products and antimicrobial sensitivity tests were done. The collected data were entered and analyzed by SPSS statistical software version 20. Binary and multiple logistic regression analysis were done to see different factors and p-values less than 0.05 were taken as statistically significant.Results: A total of three hundred seventy six (376) units of blood and blood product samples, 188 from non-diverging and 188 from diverging blood donor collection methods were included. Among different blood group types, O+ blood and the majority of components were stored from one to five days. The overall bacterial contamination among blood and blood components were n = 17 (4.5%). Among them, n = 14 (7.4%) and n = 3 (1.6%) were collected with a non-diverging and diverging method, respectively. Staphylococcus epidermidis was the most dominant isolate. Most bacterial isolates were susceptible to different antimicrobial agents; however, Pseudomonas aeruginosa showed resistance for gentamicin. The non-diverging collection units of blood and components were significantly associated with bacterial contamination. Of all, n = 5/17 (29.4%) isolated bacteria were multidrug resistant.Conclusion: The bacterial contamination of blood and blood components collected with diverging method was lower and recommended to use this collection method from donors.Keywords: bacterial contamination, diverging and non-diverging method, blood culture, susceptibility patternhttps://www.dovepress.com/bacterial-contamination-and-susceptibility-pattern-among-blood-and-blo-peer-reviewed-fulltext-article-IDRbacterial contaminationdiverging and non-diverging methodblood culturesusceptibility pattern. |
| spellingShingle | Tsegaye W Bitew A Gize A Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia Infection and Drug Resistance bacterial contamination diverging and non-diverging method blood culture susceptibility pattern. |
| title | Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia |
| title_full | Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia |
| title_fullStr | Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia |
| title_full_unstemmed | Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia |
| title_short | Bacterial Contamination and Susceptibility Pattern Among Blood and Blood Components Using Divergent and Non-Divergent Collection Methods at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia |
| title_sort | bacterial contamination and susceptibility pattern among blood and blood components using divergent and non divergent collection methods at armed forces comprehensive specialized hospital addis ababa ethiopia |
| topic | bacterial contamination diverging and non-diverging method blood culture susceptibility pattern. |
| url | https://www.dovepress.com/bacterial-contamination-and-susceptibility-pattern-among-blood-and-blo-peer-reviewed-fulltext-article-IDR |
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