Multimodal label-free imaging of living dermal equivalents including dermal papilla cells
Abstract Background Despite the significant progress in the development of skin equivalents (SEs), the problem of noninvasively assessing the quality of the cell components and the collagen structure of living SEs both before and after transplantation remains. Undoubted preference is given to in viv...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-018-0838-9 |
| _version_ | 1818505797911445504 |
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| author | Aleksandra V. Meleshina Olga S. Rogovaya Varvara V. Dudenkova Marina A. Sirotkina Maria M. Lukina Alena S. Bystrova Victoria G. Krut Daria S. Kuznetsova Ekaterina P. Kalabusheva Andrey V. Vasiliev Ekaterina A. Vorotelyak Elena V. Zagaynova |
| author_facet | Aleksandra V. Meleshina Olga S. Rogovaya Varvara V. Dudenkova Marina A. Sirotkina Maria M. Lukina Alena S. Bystrova Victoria G. Krut Daria S. Kuznetsova Ekaterina P. Kalabusheva Andrey V. Vasiliev Ekaterina A. Vorotelyak Elena V. Zagaynova |
| author_sort | Aleksandra V. Meleshina |
| collection | DOAJ |
| description | Abstract Background Despite the significant progress in the development of skin equivalents (SEs), the problem of noninvasively assessing the quality of the cell components and the collagen structure of living SEs both before and after transplantation remains. Undoubted preference is given to in vivo methods of noninvasive, label-free monitoring of the state of the SEs. Optical bioimaging methods, such as cross-polarization optical coherence tomography (CP OCT), multiphoton tomography (MPT), and fluorescence lifetime imaging microscopy (FLIM), present particular advantages for the visualization of such SEs. Methods In this study, we simultaneously applied several visualization techniques for skin model examination. We investigated the structure and quality of dermal equivalents containing dermal papilla (DP) cells and dermal fibroblasts (FBs) using CP OCT, MPT, and FLIM. Both the energy metabolism of the cell components and the structuring of the collagen fibrils were addressed. Results Based on the data from the fluorescence lifetimes and the contributions of protein-bound NAD(P)H, a bias toward oxidative metabolism was indicated, for the first time, in both the DP cells and FBs on day 14 of SE cultivation. The CP OCT and MPT data also indicated that both DP cells and FBs structured the collagen gel in a similar manner. Conclusion In this study, multimodal label-free imaging of the structure and quality of living dermal equivalents was implemented for the first time with the use CP OCT, MPT, and FLIM of NAD(P)H. Our data suggest that the combination of different imaging techniques provides an integrated approach to data acquisition regarding the structure and quality of dermal equivalents, minimizes the potential disadvantages of using a single method, and provides an ideal information profile for clinical and research applications. |
| first_indexed | 2024-12-10T21:55:47Z |
| format | Article |
| id | doaj.art-affb4d0d2b2f4aa882f82d7de7e3a67a |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-10T21:55:47Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-affb4d0d2b2f4aa882f82d7de7e3a67a2022-12-22T01:32:02ZengBMCStem Cell Research & Therapy1757-65122018-04-019111210.1186/s13287-018-0838-9Multimodal label-free imaging of living dermal equivalents including dermal papilla cellsAleksandra V. Meleshina0Olga S. Rogovaya1Varvara V. Dudenkova2Marina A. Sirotkina3Maria M. Lukina4Alena S. Bystrova5Victoria G. Krut6Daria S. Kuznetsova7Ekaterina P. Kalabusheva8Andrey V. Vasiliev9Ekaterina A. Vorotelyak10Elena V. Zagaynova11Institute of Biomedical Technologies, Nizhny Novgorod State Medical AcademyKoltzov Institute of Developmental Biology, Russian Academy of SciencesInstitute of Biomedical Technologies, Nizhny Novgorod State Medical AcademyInstitute of Biomedical Technologies, Nizhny Novgorod State Medical AcademyInstitute of Biomedical Technologies, Nizhny Novgorod State Medical AcademyInstitute of Biomedical Technologies, Nizhny Novgorod State Medical AcademyInstitute of Biology and Biomedicine, Nizhny Novgorod State UniversityInstitute of Biomedical Technologies, Nizhny Novgorod State Medical AcademyKoltzov Institute of Developmental Biology, Russian Academy of SciencesKoltzov Institute of Developmental Biology, Russian Academy of SciencesKoltzov Institute of Developmental Biology, Russian Academy of SciencesInstitute of Biomedical Technologies, Nizhny Novgorod State Medical AcademyAbstract Background Despite the significant progress in the development of skin equivalents (SEs), the problem of noninvasively assessing the quality of the cell components and the collagen structure of living SEs both before and after transplantation remains. Undoubted preference is given to in vivo methods of noninvasive, label-free monitoring of the state of the SEs. Optical bioimaging methods, such as cross-polarization optical coherence tomography (CP OCT), multiphoton tomography (MPT), and fluorescence lifetime imaging microscopy (FLIM), present particular advantages for the visualization of such SEs. Methods In this study, we simultaneously applied several visualization techniques for skin model examination. We investigated the structure and quality of dermal equivalents containing dermal papilla (DP) cells and dermal fibroblasts (FBs) using CP OCT, MPT, and FLIM. Both the energy metabolism of the cell components and the structuring of the collagen fibrils were addressed. Results Based on the data from the fluorescence lifetimes and the contributions of protein-bound NAD(P)H, a bias toward oxidative metabolism was indicated, for the first time, in both the DP cells and FBs on day 14 of SE cultivation. The CP OCT and MPT data also indicated that both DP cells and FBs structured the collagen gel in a similar manner. Conclusion In this study, multimodal label-free imaging of the structure and quality of living dermal equivalents was implemented for the first time with the use CP OCT, MPT, and FLIM of NAD(P)H. Our data suggest that the combination of different imaging techniques provides an integrated approach to data acquisition regarding the structure and quality of dermal equivalents, minimizes the potential disadvantages of using a single method, and provides an ideal information profile for clinical and research applications.http://link.springer.com/article/10.1186/s13287-018-0838-9Dermal equivalentsDermal papilla cellsNAD(P)HCP OCTMPTFLIM |
| spellingShingle | Aleksandra V. Meleshina Olga S. Rogovaya Varvara V. Dudenkova Marina A. Sirotkina Maria M. Lukina Alena S. Bystrova Victoria G. Krut Daria S. Kuznetsova Ekaterina P. Kalabusheva Andrey V. Vasiliev Ekaterina A. Vorotelyak Elena V. Zagaynova Multimodal label-free imaging of living dermal equivalents including dermal papilla cells Stem Cell Research & Therapy Dermal equivalents Dermal papilla cells NAD(P)H CP OCT MPT FLIM |
| title | Multimodal label-free imaging of living dermal equivalents including dermal papilla cells |
| title_full | Multimodal label-free imaging of living dermal equivalents including dermal papilla cells |
| title_fullStr | Multimodal label-free imaging of living dermal equivalents including dermal papilla cells |
| title_full_unstemmed | Multimodal label-free imaging of living dermal equivalents including dermal papilla cells |
| title_short | Multimodal label-free imaging of living dermal equivalents including dermal papilla cells |
| title_sort | multimodal label free imaging of living dermal equivalents including dermal papilla cells |
| topic | Dermal equivalents Dermal papilla cells NAD(P)H CP OCT MPT FLIM |
| url | http://link.springer.com/article/10.1186/s13287-018-0838-9 |
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