Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study
OBJECTIVES: This study evaluated in vitro the efficiency of Aloe vera and propolis dentifrice on reducing the contamination of toothbrush bristles by a standard strain of Streptococcus mutans (ATCC 25175; SM), after toothbrushing. MATERIAL AND METHODS: Fifteen sterile toothbrushes were randomly divi...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2012-02-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000100007 |
| _version_ | 1811282963919273984 |
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| author | Patrícia Fernanda Roesler Bertolini Oswaldo Biondi Filho Arnaldo Pomilio Sérgio Luiz Pinheiro Meghi Silva de Carvalho |
| author_facet | Patrícia Fernanda Roesler Bertolini Oswaldo Biondi Filho Arnaldo Pomilio Sérgio Luiz Pinheiro Meghi Silva de Carvalho |
| author_sort | Patrícia Fernanda Roesler Bertolini |
| collection | DOAJ |
| description | OBJECTIVES: This study evaluated in vitro the efficiency of Aloe vera and propolis dentifrice on reducing the contamination of toothbrush bristles by a standard strain of Streptococcus mutans (ATCC 25175; SM), after toothbrushing. MATERIAL AND METHODS: Fifteen sterile toothbrushes were randomly divided into 5 toothbrushing groups: I (negative control): without dentifrice; II: with fluoridated dentifrice; III: with triclosan and gantrez dentifrice; IV (positive control): without dentifrice and irrigation with 10 mL of 0.12% chlorhexidine gluconate; V: with Aloe vera and propolis dentifrice. In each group, 1 sterile bovine tooth was brushed for 1 min, where the toothbrush bristles were contaminated with 25 µL of SM. After toothbrushing, the bristles were stored in individual test tubes with 3 mL of BHI under anaerobiosis of 37°C for 48 h. Then, they were seeded with sterile swab in triplicate in the Mitis salivarius - Bacitracin culture medium. The samples were kept under anaerobiosis of 37°C for 48 h. Scores were used to count the number of colony forming units (cfu). The results were submitted to the Mann-Whitney statistical test at 5% significance level. RESULTS: There was statistically significant difference (p<0.05) for the reduction of bristle contamination comparing groups II, III, IV and V to group I. CONCLUSIONS: It may be stated that after toothbrushing, the Aloe vera and propolis dentifrice reduced the contamination of toothbrush bristles by SM, without differentiation from the other chemical agents used. |
| first_indexed | 2024-04-13T02:03:06Z |
| format | Article |
| id | doaj.art-affb9d5bf02d47d19ac97de69839bee3 |
| institution | Directory Open Access Journal |
| issn | 1678-7757 1678-7765 |
| language | English |
| last_indexed | 2024-04-13T02:03:06Z |
| publishDate | 2012-02-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-affb9d5bf02d47d19ac97de69839bee32022-12-22T03:07:34ZengUniversity of São PauloJournal of Applied Oral Science1678-77571678-77652012-02-01201323710.1590/S1678-77572012000100007Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro studyPatrícia Fernanda Roesler BertoliniOswaldo Biondi FilhoArnaldo PomilioSérgio Luiz PinheiroMeghi Silva de CarvalhoOBJECTIVES: This study evaluated in vitro the efficiency of Aloe vera and propolis dentifrice on reducing the contamination of toothbrush bristles by a standard strain of Streptococcus mutans (ATCC 25175; SM), after toothbrushing. MATERIAL AND METHODS: Fifteen sterile toothbrushes were randomly divided into 5 toothbrushing groups: I (negative control): without dentifrice; II: with fluoridated dentifrice; III: with triclosan and gantrez dentifrice; IV (positive control): without dentifrice and irrigation with 10 mL of 0.12% chlorhexidine gluconate; V: with Aloe vera and propolis dentifrice. In each group, 1 sterile bovine tooth was brushed for 1 min, where the toothbrush bristles were contaminated with 25 µL of SM. After toothbrushing, the bristles were stored in individual test tubes with 3 mL of BHI under anaerobiosis of 37°C for 48 h. Then, they were seeded with sterile swab in triplicate in the Mitis salivarius - Bacitracin culture medium. The samples were kept under anaerobiosis of 37°C for 48 h. Scores were used to count the number of colony forming units (cfu). The results were submitted to the Mann-Whitney statistical test at 5% significance level. RESULTS: There was statistically significant difference (p<0.05) for the reduction of bristle contamination comparing groups II, III, IV and V to group I. CONCLUSIONS: It may be stated that after toothbrushing, the Aloe vera and propolis dentifrice reduced the contamination of toothbrush bristles by SM, without differentiation from the other chemical agents used.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000100007DentifricesToothbrushingStreptococcus mutans |
| spellingShingle | Patrícia Fernanda Roesler Bertolini Oswaldo Biondi Filho Arnaldo Pomilio Sérgio Luiz Pinheiro Meghi Silva de Carvalho Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study Journal of Applied Oral Science Dentifrices Toothbrushing Streptococcus mutans |
| title | Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study |
| title_full | Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study |
| title_fullStr | Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study |
| title_full_unstemmed | Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study |
| title_short | Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study |
| title_sort | antimicrobial capacity of aloe vera and propolis dentifrice against streptococcus mutans strains in toothbrushes an in vitro study |
| topic | Dentifrices Toothbrushing Streptococcus mutans |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572012000100007 |
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