In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranes
<p>Abstract</p> <p>Background</p> <p>Chorioamniotic membranes infection is a pathologic condition in which an abnormal secretion of proinflammatory cytokines halts fetal immune tolerance. The aim of the present study was to evaluate the functional response of human chor...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-12-01
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| Series: | Reproductive Biology and Endocrinology |
| Online Access: | http://www.rbej.com/content/5/1/46 |
| _version_ | 1818701665186873344 |
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| author | Maida-Claros Rolando Soriano-Becerril Diana Flores-Pliego Arturo Espejel-Nuñez Aurora Flores-Herrera Hector Garcia-Lopez Guadalupe Zaga-Clavellina Veronica Merchant-Larios Horacio Vadillo-Ortega Felipe |
| author_facet | Maida-Claros Rolando Soriano-Becerril Diana Flores-Pliego Arturo Espejel-Nuñez Aurora Flores-Herrera Hector Garcia-Lopez Guadalupe Zaga-Clavellina Veronica Merchant-Larios Horacio Vadillo-Ortega Felipe |
| author_sort | Maida-Claros Rolando |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Chorioamniotic membranes infection is a pathologic condition in which an abnormal secretion of proinflammatory cytokines halts fetal immune tolerance. The aim of the present study was to evaluate the functional response of human chorioamniotic membranes, as well as the individual contribution of the amnion and choriodecidua after stimulation with Escherichia coli, a pathogen associated with preterm labor.</p> <p>Methods</p> <p>Explants of chorioamniotic membranes from 10 women (37–40 weeks of gestation) were mounted and cultured in a Transwell system, which allowed us to test the amnion and choriodecidua compartments independently. Escherichia coli (1 × 10 6 CFU/mL) was added to either the amniotic or the choriodecidual regions or both; after a 24-h incubation, the secretion of IL-1beta, IL-6, TNFalpha, IL-8, and IL-10 in both compartments was measured using a specific ELISA. Data were analyzed by Kruskal-Wallis one-way analysis of variance.</p> <p>Results</p> <p>After stimulation with Escherichia coli, the choriodecidua compartment showed an increase in the secretion of IL-1beta (21-fold), IL-6 (2-fold), IL-8 (6-fold), and IL-10 (37-fold), regardless of which side of the membrane was stimulated; TNFalpha secretion augmented (22-fold) also but only when the stimulus was applied simultaneously to both sides. When the amnion was stimulated directly, the level of IL-1beta (13-fold) rose significantly; however, the increase in IL-8 secretion was larger (20-fold), regardless of the primary site of infection. TNFalpha secretion in the amnion compartment rose markedly only when Escherichia coli was simultaneously applied to both sides.</p> <p>Conclusion</p> <p>Selective stimulation of fetal membranes with Escherichia coli results in a differential production of IL-1beta, IL-6, TNFalpha, IL-8, and IL-10. These tissues were less responsive when the amnion side was stimulated. This is in agreement with the hypothesis that the choriodecidua may play a primary role during an ascending intrauterine infection, being the main barrier to progression of the infection into the amniotic cavity. Therefore, the tissue-specific capacities for the secretion of these immune modulators could be a determining factor for the degree of severity of the inflammation process in fetal membranes.</p> |
| first_indexed | 2024-12-17T15:24:27Z |
| format | Article |
| id | doaj.art-b00004d030b941ab88ff3b49d765deab |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-17T15:24:27Z |
| publishDate | 2007-12-01 |
| publisher | BMC |
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| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-b00004d030b941ab88ff3b49d765deab2022-12-21T21:43:19ZengBMCReproductive Biology and Endocrinology1477-78272007-12-01514610.1186/1477-7827-5-46In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranesMaida-Claros RolandoSoriano-Becerril DianaFlores-Pliego ArturoEspejel-Nuñez AuroraFlores-Herrera HectorGarcia-Lopez GuadalupeZaga-Clavellina VeronicaMerchant-Larios HoracioVadillo-Ortega Felipe<p>Abstract</p> <p>Background</p> <p>Chorioamniotic membranes infection is a pathologic condition in which an abnormal secretion of proinflammatory cytokines halts fetal immune tolerance. The aim of the present study was to evaluate the functional response of human chorioamniotic membranes, as well as the individual contribution of the amnion and choriodecidua after stimulation with Escherichia coli, a pathogen associated with preterm labor.</p> <p>Methods</p> <p>Explants of chorioamniotic membranes from 10 women (37–40 weeks of gestation) were mounted and cultured in a Transwell system, which allowed us to test the amnion and choriodecidua compartments independently. Escherichia coli (1 × 10 6 CFU/mL) was added to either the amniotic or the choriodecidual regions or both; after a 24-h incubation, the secretion of IL-1beta, IL-6, TNFalpha, IL-8, and IL-10 in both compartments was measured using a specific ELISA. Data were analyzed by Kruskal-Wallis one-way analysis of variance.</p> <p>Results</p> <p>After stimulation with Escherichia coli, the choriodecidua compartment showed an increase in the secretion of IL-1beta (21-fold), IL-6 (2-fold), IL-8 (6-fold), and IL-10 (37-fold), regardless of which side of the membrane was stimulated; TNFalpha secretion augmented (22-fold) also but only when the stimulus was applied simultaneously to both sides. When the amnion was stimulated directly, the level of IL-1beta (13-fold) rose significantly; however, the increase in IL-8 secretion was larger (20-fold), regardless of the primary site of infection. TNFalpha secretion in the amnion compartment rose markedly only when Escherichia coli was simultaneously applied to both sides.</p> <p>Conclusion</p> <p>Selective stimulation of fetal membranes with Escherichia coli results in a differential production of IL-1beta, IL-6, TNFalpha, IL-8, and IL-10. These tissues were less responsive when the amnion side was stimulated. This is in agreement with the hypothesis that the choriodecidua may play a primary role during an ascending intrauterine infection, being the main barrier to progression of the infection into the amniotic cavity. Therefore, the tissue-specific capacities for the secretion of these immune modulators could be a determining factor for the degree of severity of the inflammation process in fetal membranes.</p>http://www.rbej.com/content/5/1/46 |
| spellingShingle | Maida-Claros Rolando Soriano-Becerril Diana Flores-Pliego Arturo Espejel-Nuñez Aurora Flores-Herrera Hector Garcia-Lopez Guadalupe Zaga-Clavellina Veronica Merchant-Larios Horacio Vadillo-Ortega Felipe In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranes Reproductive Biology and Endocrinology |
| title | In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranes |
| title_full | In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranes |
| title_fullStr | In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranes |
| title_full_unstemmed | In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranes |
| title_short | In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with <it>Escherichia coli</it> in human fetal membranes |
| title_sort | in vitro secretion profiles of interleukin il 1beta il 6 il 8 il 10 and tnf alpha after selective infection with it escherichia coli it in human fetal membranes |
| url | http://www.rbej.com/content/5/1/46 |
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