In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array
Chemiluminescence (CL) detection is widely employed in biosensors and miniaturized analytical devices since it offers high detectability and flexible device design (there are no geometry requirements for the measurement cell, except the ability to collect the largest fraction of emitted photons). Al...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/8/1/18 |
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| author | Simone Berneschi Cosimo Trono Mara Mirasoli Ambra Giannetti Martina Zangheri Massimo Guardigli Sara Tombelli Elisa Marchegiani Francesco Baldini Aldo Roda |
| author_facet | Simone Berneschi Cosimo Trono Mara Mirasoli Ambra Giannetti Martina Zangheri Massimo Guardigli Sara Tombelli Elisa Marchegiani Francesco Baldini Aldo Roda |
| author_sort | Simone Berneschi |
| collection | DOAJ |
| description | Chemiluminescence (CL) detection is widely employed in biosensors and miniaturized analytical devices since it offers high detectability and flexible device design (there are no geometry requirements for the measurement cell, except the ability to collect the largest fraction of emitted photons). Although the emission anisotropy phenomenon for an emitting dipole bound to the interface between two media with different refractive index is well known for fluorescence, it is still poorly investigated for CL reactions, in which the excited-state reaction products can diffuse in solution before the photon emission event. In this paper, we propose a simple method for the real-time evaluation of the CL emission anisotropy based on a radial array of optical fibers, embedded in a poly(methyl methacrylate) semicylinder and coupled with a Charge-Coupled Device (CCD) camera through a suitable interface. The polar-time evolutions of the CL emission have been studied for catalyzing enzymes immobilized onto a solid surface (heterogeneous configuration) or free in solution (homogeneous configuration). Evidence of the anisotropy phenomenon is observed, indicating that the lifetime of the excited-state products of the enzyme-catalyzed reactions is shorter than the time required for their diffusion in solution at a distance at which the CL can be considered isotropic. These results open new perspectives in the development of CL-based miniaturized analytical devices. |
| first_indexed | 2024-04-12T20:07:14Z |
| format | Article |
| id | doaj.art-b0003ce4596e45779ebe5c35a48af152 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-04-12T20:07:14Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-b0003ce4596e45779ebe5c35a48af1522022-12-22T03:18:22ZengMDPI AGChemosensors2227-90402020-02-01811810.3390/chemosensors8010018chemosensors8010018In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial ArraySimone Berneschi0Cosimo Trono1Mara Mirasoli2Ambra Giannetti3Martina Zangheri4Massimo Guardigli5Sara Tombelli6Elisa Marchegiani7Francesco Baldini8Aldo Roda9Institute of Applied Physics “Nello Carrara”, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyInstitute of Applied Physics “Nello Carrara”, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, via Selmi 2, 40126 Bologna (BO), ItalyInstitute of Applied Physics “Nello Carrara”, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, via Selmi 2, 40126 Bologna (BO), ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, via Selmi 2, 40126 Bologna (BO), ItalyInstitute of Applied Physics “Nello Carrara”, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, via Selmi 2, 40126 Bologna (BO), ItalyInstitute of Applied Physics “Nello Carrara”, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyDepartment of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, via Selmi 2, 40126 Bologna (BO), ItalyChemiluminescence (CL) detection is widely employed in biosensors and miniaturized analytical devices since it offers high detectability and flexible device design (there are no geometry requirements for the measurement cell, except the ability to collect the largest fraction of emitted photons). Although the emission anisotropy phenomenon for an emitting dipole bound to the interface between two media with different refractive index is well known for fluorescence, it is still poorly investigated for CL reactions, in which the excited-state reaction products can diffuse in solution before the photon emission event. In this paper, we propose a simple method for the real-time evaluation of the CL emission anisotropy based on a radial array of optical fibers, embedded in a poly(methyl methacrylate) semicylinder and coupled with a Charge-Coupled Device (CCD) camera through a suitable interface. The polar-time evolutions of the CL emission have been studied for catalyzing enzymes immobilized onto a solid surface (heterogeneous configuration) or free in solution (homogeneous configuration). Evidence of the anisotropy phenomenon is observed, indicating that the lifetime of the excited-state products of the enzyme-catalyzed reactions is shorter than the time required for their diffusion in solution at a distance at which the CL can be considered isotropic. These results open new perspectives in the development of CL-based miniaturized analytical devices.https://www.mdpi.com/2227-9040/8/1/18chemiluminescenceemission anisotropymultimode optical fiberccd camera detector |
| spellingShingle | Simone Berneschi Cosimo Trono Mara Mirasoli Ambra Giannetti Martina Zangheri Massimo Guardigli Sara Tombelli Elisa Marchegiani Francesco Baldini Aldo Roda In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array Chemosensors chemiluminescence emission anisotropy multimode optical fiber ccd camera detector |
| title | In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array |
| title_full | In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array |
| title_fullStr | In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array |
| title_full_unstemmed | In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array |
| title_short | In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array |
| title_sort | in parallel polar monitoring of chemiluminescence emission anisotropy at the solid liquid interface by an optical fiber radial array |
| topic | chemiluminescence emission anisotropy multimode optical fiber ccd camera detector |
| url | https://www.mdpi.com/2227-9040/8/1/18 |
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