Mycotic Keratitis in Solapur (A Two Years Study)
ntroduction: Corneal ulcer is the second most common cause of visual disability and blindness in developing countries after cataract. Corneal scarring due to keratitis can be prevented. This therefore necessitates the knowledge of its etiology for early intervention. Aim: To study the occurrenc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Pvt. Ltd.
2016-04-01
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| Series: | National Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.njlm.net/articles/PDF/2108/7-%2017664_F(P)_PF1(Vsu_Om)_PFA(Om)_PF2(PVSU).pdf |
| _version_ | 1818808091251048448 |
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| author | Karan Indra Ostwal Rebecca Lalngaihzuali Nasira Khalid Shaikh Kishore Ingole Rajaram Powar |
| author_facet | Karan Indra Ostwal Rebecca Lalngaihzuali Nasira Khalid Shaikh Kishore Ingole Rajaram Powar |
| author_sort | Karan Indra Ostwal |
| collection | DOAJ |
| description | ntroduction: Corneal ulcer is the second most common
cause of visual disability and blindness in developing
countries after cataract. Corneal scarring due to keratitis can
be prevented. This therefore necessitates the knowledge of
its etiology for early intervention.
Aim: To study the occurrence, etiology and predisposing
factors of mycotic keratitis at a tertiary care centre in
Solapur, Maharashtra.
Materials and Methods: The retrospective study was
carried out for duration of 2 years (April 2013 to March
2015). All aseptically collected corneal scrapings from
clinically suspected cases of fungal keratitis were included.
Conventional methods i.e. KOH mount, Gram’s stain and
fungal cultures on a pair of Saubaraud Dextrose Agar were
used for diagnosis. Slide cultures on Corn Meal Agar with
tween 80 were used for confirmation of moulds and germ
tube tests done for yeasts.
Results: Hundred corneal scrapings were studied out of
which fungal hyphae were identified in 20% of the KOH
mounts and in 17% of Gram’s stained smears. Fungal
culture was positive in 24 samples. Predominant isolate was
Fusarium spp (29%. 7/24) followed by Alternaria spp (21%,
5/24) and Aspergillus spp (17%, 4/24) and 8% (2/24) each
of Cladosporium spp, Penicillium spp, Bipolaris spp and
Candida albicans. Ocular injury was the most commonly
encountered predisposing factor.
Conclusion: Fungal aetiology constitutes a significant
proportion of corneal ulcers, out of which Fusarium spp is
the most common isolate in Solapur, Maharashtra followed
by Alternaria spp unlike majority of studies in the country
where Aspergillus spp are the most common isolates. |
| first_indexed | 2024-12-18T19:36:03Z |
| format | Article |
| id | doaj.art-b0063f0af8e5477b90e9f0a7ba0c94e8 |
| institution | Directory Open Access Journal |
| issn | 2277-8551 2455-6882 |
| language | English |
| last_indexed | 2024-12-18T19:36:03Z |
| publishDate | 2016-04-01 |
| publisher | JCDR Research and Publications Pvt. Ltd. |
| record_format | Article |
| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-b0063f0af8e5477b90e9f0a7ba0c94e82022-12-21T20:55:36ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822016-04-0152303310.7860/NJLM/2016/17664:2108Mycotic Keratitis in Solapur (A Two Years Study)Karan Indra Ostwal0Rebecca Lalngaihzuali1Nasira Khalid Shaikh2Kishore Ingole3Rajaram Powar4Assistant Professor, Department of Microbiology, Government Medical College, Akola, India.Junior Resident, Department of Microbiology, Dr. VMGMC Solapur, India.Associate Professor, Department of Microbiology, Dr. VMGMC Solapur, India.Professor and Head, Department of Microbiology, Dr. VMGMC Solapur, India.Dean, Department of Microbiology, Dr. VMGMC, Solapur, India.ntroduction: Corneal ulcer is the second most common cause of visual disability and blindness in developing countries after cataract. Corneal scarring due to keratitis can be prevented. This therefore necessitates the knowledge of its etiology for early intervention. Aim: To study the occurrence, etiology and predisposing factors of mycotic keratitis at a tertiary care centre in Solapur, Maharashtra. Materials and Methods: The retrospective study was carried out for duration of 2 years (April 2013 to March 2015). All aseptically collected corneal scrapings from clinically suspected cases of fungal keratitis were included. Conventional methods i.e. KOH mount, Gram’s stain and fungal cultures on a pair of Saubaraud Dextrose Agar were used for diagnosis. Slide cultures on Corn Meal Agar with tween 80 were used for confirmation of moulds and germ tube tests done for yeasts. Results: Hundred corneal scrapings were studied out of which fungal hyphae were identified in 20% of the KOH mounts and in 17% of Gram’s stained smears. Fungal culture was positive in 24 samples. Predominant isolate was Fusarium spp (29%. 7/24) followed by Alternaria spp (21%, 5/24) and Aspergillus spp (17%, 4/24) and 8% (2/24) each of Cladosporium spp, Penicillium spp, Bipolaris spp and Candida albicans. Ocular injury was the most commonly encountered predisposing factor. Conclusion: Fungal aetiology constitutes a significant proportion of corneal ulcers, out of which Fusarium spp is the most common isolate in Solapur, Maharashtra followed by Alternaria spp unlike majority of studies in the country where Aspergillus spp are the most common isolates.http://www.njlm.net/articles/PDF/2108/7-%2017664_F(P)_PF1(Vsu_Om)_PFA(Om)_PF2(PVSU).pdfcorneal scrapingsfungal culturefungal isolatesmicroscopy |
| spellingShingle | Karan Indra Ostwal Rebecca Lalngaihzuali Nasira Khalid Shaikh Kishore Ingole Rajaram Powar Mycotic Keratitis in Solapur (A Two Years Study) National Journal of Laboratory Medicine corneal scrapings fungal culture fungal isolates microscopy |
| title | Mycotic Keratitis in Solapur (A Two Years Study) |
| title_full | Mycotic Keratitis in Solapur (A Two Years Study) |
| title_fullStr | Mycotic Keratitis in Solapur (A Two Years Study) |
| title_full_unstemmed | Mycotic Keratitis in Solapur (A Two Years Study) |
| title_short | Mycotic Keratitis in Solapur (A Two Years Study) |
| title_sort | mycotic keratitis in solapur a two years study |
| topic | corneal scrapings fungal culture fungal isolates microscopy |
| url | http://www.njlm.net/articles/PDF/2108/7-%2017664_F(P)_PF1(Vsu_Om)_PFA(Om)_PF2(PVSU).pdf |
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