Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin Cells
Selaginellaceae plants are used in cosmetics to limit skin aging. This study is the first to investigate the anti-aging effects of <i>Selaginella rossii</i> (SR) on ultraviolet B (UVB)- and oxidative stress-induced skin cells. The 95% ethanol extract of <i>Selaginella rossii</i&...
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MDPI AG
2022-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/12/2106 |
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| author | Hwa Lee Soo-Yong Kim Sang Woo Lee Sehan Kwak Hulin Li Renzhe Piao Ho-Yong Park Sangho Choi Tae-Sook Jeong |
| author_facet | Hwa Lee Soo-Yong Kim Sang Woo Lee Sehan Kwak Hulin Li Renzhe Piao Ho-Yong Park Sangho Choi Tae-Sook Jeong |
| author_sort | Hwa Lee |
| collection | DOAJ |
| description | Selaginellaceae plants are used in cosmetics to limit skin aging. This study is the first to investigate the anti-aging effects of <i>Selaginella rossii</i> (SR) on ultraviolet B (UVB)- and oxidative stress-induced skin cells. The 95% ethanol extract of <i>Selaginella rossii</i> (SR95E) contained much higher amounts of amentoflavone (AMF), an active compound, than other Selaginellaceae plants and was more effective in inhibiting matrix metalloproteinase (MMP)-1 expression in CCD-986sk fibroblasts. SR95E significantly decreased UVB-induced <i>MMP-1</i>, <i>MMP-2</i>, <i>MMP-3</i> and <i>MMP-9</i> expression and enhanced procollagen type I C-peptide content and mRNA expression of collagen type I alpha (<i>COL1A)1</i> and <i>COL1A2</i> in CCD-986sk fibroblasts. In HaCaT keratinocytes, SR95E treatment also dose-dependently decreased UVB-induced MMP-1 concentration and <i>MMP-1</i>, <i>MMP-2</i>, <i>MMP-3</i> and <i>MMP-9</i> mRNA expression. Moreover, SR95E treatment markedly inhibited UVB-induced c-Jun N-terminal kinase and p38 mitogen-activated protein kinase signaling and nuclear factor kappa-B signaling in HaCaT cells. Furthermore, SR95E and AMF markedly regulated the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced expression of cellular senescence-related markers, including <i>p16</i>, <i>p21</i> and <i>LMNB1</i>, in HaCaT cells. Overall, this study indicates that SR may have potential as a functional material on preventing UVB- and AAPH-induced skin aging and wrinkles. |
| first_indexed | 2024-03-09T16:11:16Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:11:16Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-b008f77cb6f14689b4a83bd05eb27a662023-11-24T16:13:39ZengMDPI AGLife2075-17292022-12-011212210610.3390/life12122106Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin CellsHwa Lee0Soo-Yong Kim1Sang Woo Lee2Sehan Kwak3Hulin Li4Renzhe Piao5Ho-Yong Park6Sangho Choi7Tae-Sook Jeong8Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaInternational Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaInternational Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaMicrobiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaDepartment of Agronomy, Yanbian University Agriculture College, Yanji 133000, ChinaDepartment of Agronomy, Yanbian University Agriculture College, Yanji 133000, ChinaMicrobiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaInternational Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaMicrobiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of KoreaSelaginellaceae plants are used in cosmetics to limit skin aging. This study is the first to investigate the anti-aging effects of <i>Selaginella rossii</i> (SR) on ultraviolet B (UVB)- and oxidative stress-induced skin cells. The 95% ethanol extract of <i>Selaginella rossii</i> (SR95E) contained much higher amounts of amentoflavone (AMF), an active compound, than other Selaginellaceae plants and was more effective in inhibiting matrix metalloproteinase (MMP)-1 expression in CCD-986sk fibroblasts. SR95E significantly decreased UVB-induced <i>MMP-1</i>, <i>MMP-2</i>, <i>MMP-3</i> and <i>MMP-9</i> expression and enhanced procollagen type I C-peptide content and mRNA expression of collagen type I alpha (<i>COL1A)1</i> and <i>COL1A2</i> in CCD-986sk fibroblasts. In HaCaT keratinocytes, SR95E treatment also dose-dependently decreased UVB-induced MMP-1 concentration and <i>MMP-1</i>, <i>MMP-2</i>, <i>MMP-3</i> and <i>MMP-9</i> mRNA expression. Moreover, SR95E treatment markedly inhibited UVB-induced c-Jun N-terminal kinase and p38 mitogen-activated protein kinase signaling and nuclear factor kappa-B signaling in HaCaT cells. Furthermore, SR95E and AMF markedly regulated the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced expression of cellular senescence-related markers, including <i>p16</i>, <i>p21</i> and <i>LMNB1</i>, in HaCaT cells. Overall, this study indicates that SR may have potential as a functional material on preventing UVB- and AAPH-induced skin aging and wrinkles.https://www.mdpi.com/2075-1729/12/12/2106<i>Selaginella rossii</i>matrix metalloproteinasesamentoflavoneskin aginganti-wrinkle |
| spellingShingle | Hwa Lee Soo-Yong Kim Sang Woo Lee Sehan Kwak Hulin Li Renzhe Piao Ho-Yong Park Sangho Choi Tae-Sook Jeong Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin Cells Life <i>Selaginella rossii</i> matrix metalloproteinases amentoflavone skin aging anti-wrinkle |
| title | Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin Cells |
| title_full | Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin Cells |
| title_fullStr | Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin Cells |
| title_full_unstemmed | Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin Cells |
| title_short | Amentoflavone-Enriched <i>Selaginella rossii</i> Protects against Ultraviolet- and Oxidative Stress-Induced Aging in Skin Cells |
| title_sort | amentoflavone enriched i selaginella rossii i protects against ultraviolet and oxidative stress induced aging in skin cells |
| topic | <i>Selaginella rossii</i> matrix metalloproteinases amentoflavone skin aging anti-wrinkle |
| url | https://www.mdpi.com/2075-1729/12/12/2106 |
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