Regulation of Plasmodium sporozoite motility by formulation components
Abstract Background The protective efficacy of the most promising malaria whole-parasite based vaccine candidates critically depends on the parasite’s potential to migrate in the human host. Key components of the parasite motility machinery (e.g. adhesive proteins, actin/myosin-based motor, geometri...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12936-019-2794-y |
| _version_ | 1811289362495700992 |
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| author | Clarize M. de Korne Luuk T. Lageschaar Matthias N. van Oosterom Els Baalbergen Beatrice M. F. Winkel Severine C. Chevalley-Maurel Aldrik H. Velders Blandine M. D. Franke-Fayard Fijs W. B. van Leeuwen Meta Roestenberg |
| author_facet | Clarize M. de Korne Luuk T. Lageschaar Matthias N. van Oosterom Els Baalbergen Beatrice M. F. Winkel Severine C. Chevalley-Maurel Aldrik H. Velders Blandine M. D. Franke-Fayard Fijs W. B. van Leeuwen Meta Roestenberg |
| author_sort | Clarize M. de Korne |
| collection | DOAJ |
| description | Abstract Background The protective efficacy of the most promising malaria whole-parasite based vaccine candidates critically depends on the parasite’s potential to migrate in the human host. Key components of the parasite motility machinery (e.g. adhesive proteins, actin/myosin-based motor, geometrical properties) have been identified, however the regulation of this machinery is an unknown process. Methods In vitro microscopic live imaging of parasites in different formulations was performed and analysed, with the quantitative analysis software SMOOT In vitro , their motility; their adherence capacity, movement pattern and velocity during forward locomotion. Results SMOOT In vitro enabled the detailed analysis of the regulation of the motility machinery of Plasmodium berghei in response to specific (macro)molecules in the formulation. Albumin acted as an essential supplement to induce parasite attachment and movement. Glucose, salts and other whole serum components further increased the attachment rate and regulated the velocity of the movement. Conclusions Based on the findings can be concluded that a complex interplay of albumin, glucose and certain salts and amino acids regulates parasite motility. Insights in parasite motility regulation by supplements in solution potentially provide a way to optimize the whole-parasite malaria vaccine formulation. |
| first_indexed | 2024-04-13T03:53:32Z |
| format | Article |
| id | doaj.art-b00aceff87de40a1bd56a4febb838fb9 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-13T03:53:32Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-b00aceff87de40a1bd56a4febb838fb92022-12-22T03:03:42ZengBMCMalaria Journal1475-28752019-05-0118111110.1186/s12936-019-2794-yRegulation of Plasmodium sporozoite motility by formulation componentsClarize M. de Korne0Luuk T. Lageschaar1Matthias N. van Oosterom2Els Baalbergen3Beatrice M. F. Winkel4Severine C. Chevalley-Maurel5Aldrik H. Velders6Blandine M. D. Franke-Fayard7Fijs W. B. van Leeuwen8Meta Roestenberg9Department of Parasitology, Leiden University Medical CenterInterventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical CenterInterventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical CenterDepartment of Parasitology, Leiden University Medical CenterDepartment of Parasitology, Leiden University Medical CenterDepartment of Parasitology, Leiden University Medical CenterInterventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical CenterDepartment of Parasitology, Leiden University Medical CenterInterventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical CenterDepartment of Parasitology, Leiden University Medical CenterAbstract Background The protective efficacy of the most promising malaria whole-parasite based vaccine candidates critically depends on the parasite’s potential to migrate in the human host. Key components of the parasite motility machinery (e.g. adhesive proteins, actin/myosin-based motor, geometrical properties) have been identified, however the regulation of this machinery is an unknown process. Methods In vitro microscopic live imaging of parasites in different formulations was performed and analysed, with the quantitative analysis software SMOOT In vitro , their motility; their adherence capacity, movement pattern and velocity during forward locomotion. Results SMOOT In vitro enabled the detailed analysis of the regulation of the motility machinery of Plasmodium berghei in response to specific (macro)molecules in the formulation. Albumin acted as an essential supplement to induce parasite attachment and movement. Glucose, salts and other whole serum components further increased the attachment rate and regulated the velocity of the movement. Conclusions Based on the findings can be concluded that a complex interplay of albumin, glucose and certain salts and amino acids regulates parasite motility. Insights in parasite motility regulation by supplements in solution potentially provide a way to optimize the whole-parasite malaria vaccine formulation.http://link.springer.com/article/10.1186/s12936-019-2794-yMotility regulationLive in vitro imagingPlasmodium bergheiVaccine formulation |
| spellingShingle | Clarize M. de Korne Luuk T. Lageschaar Matthias N. van Oosterom Els Baalbergen Beatrice M. F. Winkel Severine C. Chevalley-Maurel Aldrik H. Velders Blandine M. D. Franke-Fayard Fijs W. B. van Leeuwen Meta Roestenberg Regulation of Plasmodium sporozoite motility by formulation components Malaria Journal Motility regulation Live in vitro imaging Plasmodium berghei Vaccine formulation |
| title | Regulation of Plasmodium sporozoite motility by formulation components |
| title_full | Regulation of Plasmodium sporozoite motility by formulation components |
| title_fullStr | Regulation of Plasmodium sporozoite motility by formulation components |
| title_full_unstemmed | Regulation of Plasmodium sporozoite motility by formulation components |
| title_short | Regulation of Plasmodium sporozoite motility by formulation components |
| title_sort | regulation of plasmodium sporozoite motility by formulation components |
| topic | Motility regulation Live in vitro imaging Plasmodium berghei Vaccine formulation |
| url | http://link.springer.com/article/10.1186/s12936-019-2794-y |
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