Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays
<p>Abstract</p> <p>Background</p> <p>Current commercial high-density oligonucleotide microarrays can hold millions of probe spots on a single microscopic glass slide and are ideal for studying the transcriptome of microbial genomes using a tiling probe design. This pape...
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| Format: | Article |
| Language: | English |
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BMC
2010-02-01
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| Series: | BMC Bioinformatics |
| Online Access: | http://www.biomedcentral.com/1471-2105/11/82 |
| _version_ | 1818807729874010112 |
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| author | Chen Tsute Høvik Hedda |
| author_facet | Chen Tsute Høvik Hedda |
| author_sort | Chen Tsute |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Current commercial high-density oligonucleotide microarrays can hold millions of probe spots on a single microscopic glass slide and are ideal for studying the transcriptome of microbial genomes using a tiling probe design. This paper describes a comprehensive computational pipeline implemented specifically for designing tiling probe sets to study microbial transcriptome profiles.</p> <p>Results</p> <p>The pipeline identifies every possible probe sequence from both forward and reverse-complement strands of all DNA sequences in the target genome including circular or linear chromosomes and plasmids. Final probe sequence lengths are adjusted based on the maximal oligonucleotide synthesis cycles and best isothermality allowed. Optimal probes are then selected in two stages - sequential and gap-filling. In the sequential stage, probes are selected from sequence windows tiled alongside the genome. In the gap-filling stage, additional probes are selected from the largest gaps between adjacent probes that have already been selected, until a predefined number of probes is reached. Selection of the highest quality probe within each window and gap is based on five criteria: sequence uniqueness, probe self-annealing, melting temperature, oligonucleotide length, and probe position.</p> <p>Conclusions</p> <p>The probe selection pipeline evaluates global and local probe sequence properties and selects a set of probes dynamically and evenly distributed along the target genome. Unique to other similar methods, an exact number of non-redundant probes can be designed to utilize all the available probe spots on any chosen microarray platform. The pipeline can be applied to microbial genomes when designing high-density tiling arrays for comparative genomics, ChIP chip, gene expression and comprehensive transcriptome studies.</p> |
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| format | Article |
| id | doaj.art-b00c08fb48ee4ff0a637f1725cfd708d |
| institution | Directory Open Access Journal |
| issn | 1471-2105 |
| language | English |
| last_indexed | 2024-12-18T19:30:18Z |
| publishDate | 2010-02-01 |
| publisher | BMC |
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| spelling | doaj.art-b00c08fb48ee4ff0a637f1725cfd708d2022-12-21T20:55:44ZengBMCBMC Bioinformatics1471-21052010-02-011118210.1186/1471-2105-11-82Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarraysChen TsuteHøvik Hedda<p>Abstract</p> <p>Background</p> <p>Current commercial high-density oligonucleotide microarrays can hold millions of probe spots on a single microscopic glass slide and are ideal for studying the transcriptome of microbial genomes using a tiling probe design. This paper describes a comprehensive computational pipeline implemented specifically for designing tiling probe sets to study microbial transcriptome profiles.</p> <p>Results</p> <p>The pipeline identifies every possible probe sequence from both forward and reverse-complement strands of all DNA sequences in the target genome including circular or linear chromosomes and plasmids. Final probe sequence lengths are adjusted based on the maximal oligonucleotide synthesis cycles and best isothermality allowed. Optimal probes are then selected in two stages - sequential and gap-filling. In the sequential stage, probes are selected from sequence windows tiled alongside the genome. In the gap-filling stage, additional probes are selected from the largest gaps between adjacent probes that have already been selected, until a predefined number of probes is reached. Selection of the highest quality probe within each window and gap is based on five criteria: sequence uniqueness, probe self-annealing, melting temperature, oligonucleotide length, and probe position.</p> <p>Conclusions</p> <p>The probe selection pipeline evaluates global and local probe sequence properties and selects a set of probes dynamically and evenly distributed along the target genome. Unique to other similar methods, an exact number of non-redundant probes can be designed to utilize all the available probe spots on any chosen microarray platform. The pipeline can be applied to microbial genomes when designing high-density tiling arrays for comparative genomics, ChIP chip, gene expression and comprehensive transcriptome studies.</p>http://www.biomedcentral.com/1471-2105/11/82 |
| spellingShingle | Chen Tsute Høvik Hedda Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays BMC Bioinformatics |
| title | Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays |
| title_full | Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays |
| title_fullStr | Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays |
| title_full_unstemmed | Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays |
| title_short | Dynamic probe selection for studying microbial transcriptome with high-density genomic tiling microarrays |
| title_sort | dynamic probe selection for studying microbial transcriptome with high density genomic tiling microarrays |
| url | http://www.biomedcentral.com/1471-2105/11/82 |
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