The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells
Adult cardiomyocytes (CMs) have very limited capacity to regenerate. Therefore, there is a great interest in developing strategies to treat infarcted CMs that are able to regenerate cardiac tissue and promote revascularization of infarcted zones in the heart. Recently, stem cell transplantation has...
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MDPI AG
2012-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/12/14975 |
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| author | Yan Wang Feng-Juan Xing Ling-Jie Meng Qiu-Ye Ji Mu-Rui Zhang Jian-Wu Zhao |
| author_facet | Yan Wang Feng-Juan Xing Ling-Jie Meng Qiu-Ye Ji Mu-Rui Zhang Jian-Wu Zhao |
| author_sort | Yan Wang |
| collection | DOAJ |
| description | Adult cardiomyocytes (CMs) have very limited capacity to regenerate. Therefore, there is a great interest in developing strategies to treat infarcted CMs that are able to regenerate cardiac tissue and promote revascularization of infarcted zones in the heart. Recently, stem cell transplantation has been proposed to replace infarcted CMs and to restore the function of the affected tissue. This area of research has become very active in recent years due to the huge clinical need to improve the efficacy of currently available therapies. Slingshot (SSH) is a family of protein phosphatases, which can specifically dephosphorylate and reactivate cofilin and inhibit the polymerization of actin filaments and actively involved in cytoskeleton rearrangement. In this study, we found that SSH1L promoted morphology changes of microfilaments during differentiation but was inhibited by the inhibitors of actin polymerization such as cytochalasin D. Overexpression of SSH1L could promote cardiac-specific protein and genes expression. 5-Aza can induce the differentiation of hMSCs into cardiomyocyte-like cells in vitro. We also observed that SSH1L efficiently promotes hMSCs differentiation into cardiomyocyte-like cells through regulation and rearrangement of cytoskeleton. Our work provides evidence that supports the positive role of SSH1L in the mechanism of stem cell differentiation into cardiomyocyte-like cells. |
| first_indexed | 2024-12-10T21:33:27Z |
| format | Article |
| id | doaj.art-b00c71ad738b4398a119ce3a6551b61e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T21:33:27Z |
| publishDate | 2012-12-01 |
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| series | Molecules |
| spelling | doaj.art-b00c71ad738b4398a119ce3a6551b61e2022-12-22T01:32:43ZengMDPI AGMolecules1420-30492012-12-011712149751499410.3390/molecules171214975The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like CellsYan WangFeng-Juan XingLing-Jie MengQiu-Ye JiMu-Rui ZhangJian-Wu ZhaoAdult cardiomyocytes (CMs) have very limited capacity to regenerate. Therefore, there is a great interest in developing strategies to treat infarcted CMs that are able to regenerate cardiac tissue and promote revascularization of infarcted zones in the heart. Recently, stem cell transplantation has been proposed to replace infarcted CMs and to restore the function of the affected tissue. This area of research has become very active in recent years due to the huge clinical need to improve the efficacy of currently available therapies. Slingshot (SSH) is a family of protein phosphatases, which can specifically dephosphorylate and reactivate cofilin and inhibit the polymerization of actin filaments and actively involved in cytoskeleton rearrangement. In this study, we found that SSH1L promoted morphology changes of microfilaments during differentiation but was inhibited by the inhibitors of actin polymerization such as cytochalasin D. Overexpression of SSH1L could promote cardiac-specific protein and genes expression. 5-Aza can induce the differentiation of hMSCs into cardiomyocyte-like cells in vitro. We also observed that SSH1L efficiently promotes hMSCs differentiation into cardiomyocyte-like cells through regulation and rearrangement of cytoskeleton. Our work provides evidence that supports the positive role of SSH1L in the mechanism of stem cell differentiation into cardiomyocyte-like cells.http://www.mdpi.com/1420-3049/17/12/14975mesenchymal stem cellscardiomyocyte-like cells5-azacytidineSlingshot-1L |
| spellingShingle | Yan Wang Feng-Juan Xing Ling-Jie Meng Qiu-Ye Ji Mu-Rui Zhang Jian-Wu Zhao The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells Molecules mesenchymal stem cells cardiomyocyte-like cells 5-azacytidine Slingshot-1L |
| title | The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells |
| title_full | The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells |
| title_fullStr | The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells |
| title_full_unstemmed | The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells |
| title_short | The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells |
| title_sort | role of slingshot 1l ssh1l in the differentiation of human bone marrow mesenchymal stem cells into cardiomyocyte like cells |
| topic | mesenchymal stem cells cardiomyocyte-like cells 5-azacytidine Slingshot-1L |
| url | http://www.mdpi.com/1420-3049/17/12/14975 |
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