Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice.
Members of the TRP superfamily of ion channels mediate mechanosensation in some organisms, and have been suggested as candidates for the mechanotransduction channel in vertebrate hair cells. Some TRP channels can be ruled out based on lack of an inner ear phenotype in knockout animals or pore proper...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155577&type=printable |
| _version_ | 1826584200413708288 |
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| author | Xudong Wu Artur A Indzhykulian Paul D Niksch Roxanna M Webber Miguel Garcia-Gonzalez Terry Watnick Jing Zhou Melissa A Vollrath David P Corey |
| author_facet | Xudong Wu Artur A Indzhykulian Paul D Niksch Roxanna M Webber Miguel Garcia-Gonzalez Terry Watnick Jing Zhou Melissa A Vollrath David P Corey |
| author_sort | Xudong Wu |
| collection | DOAJ |
| description | Members of the TRP superfamily of ion channels mediate mechanosensation in some organisms, and have been suggested as candidates for the mechanotransduction channel in vertebrate hair cells. Some TRP channels can be ruled out based on lack of an inner ear phenotype in knockout animals or pore properties not similar to the hair-cell channel. Such studies have excluded Trpv4, Trpa1, Trpml3, Trpm1, Trpm3, Trpc1, Trpc3, Trpc5, and Trpc6. However, others remain reasonable candidates. We used data from an RNA-seq analysis of gene expression in hair cells as well as data on TRP channel conductance to narrow the candidate group. We then characterized mice lacking functional Trpm2, Pkd2, Pkd2l1, Pkd2l2 and Pkd1l3, using scanning electron microscopy, auditory brainstem response, permeant dye accumulation, and single-cell electrophysiology. In all of these TRP-deficient mice, and in double and triple knockouts, mechanotransduction persisted. Together with published studies, these results argue against the participation of any of the 33 mouse TRP channels in hair cell transduction. |
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| format | Article |
| id | doaj.art-b00f9182aaf54875992b113d3ab2035d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T15:33:58Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-b00f9182aaf54875992b113d3ab2035d2025-02-25T05:34:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015557710.1371/journal.pone.0155577Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice.Xudong WuArtur A IndzhykulianPaul D NikschRoxanna M WebberMiguel Garcia-GonzalezTerry WatnickJing ZhouMelissa A VollrathDavid P CoreyMembers of the TRP superfamily of ion channels mediate mechanosensation in some organisms, and have been suggested as candidates for the mechanotransduction channel in vertebrate hair cells. Some TRP channels can be ruled out based on lack of an inner ear phenotype in knockout animals or pore properties not similar to the hair-cell channel. Such studies have excluded Trpv4, Trpa1, Trpml3, Trpm1, Trpm3, Trpc1, Trpc3, Trpc5, and Trpc6. However, others remain reasonable candidates. We used data from an RNA-seq analysis of gene expression in hair cells as well as data on TRP channel conductance to narrow the candidate group. We then characterized mice lacking functional Trpm2, Pkd2, Pkd2l1, Pkd2l2 and Pkd1l3, using scanning electron microscopy, auditory brainstem response, permeant dye accumulation, and single-cell electrophysiology. In all of these TRP-deficient mice, and in double and triple knockouts, mechanotransduction persisted. Together with published studies, these results argue against the participation of any of the 33 mouse TRP channels in hair cell transduction.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155577&type=printable |
| spellingShingle | Xudong Wu Artur A Indzhykulian Paul D Niksch Roxanna M Webber Miguel Garcia-Gonzalez Terry Watnick Jing Zhou Melissa A Vollrath David P Corey Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice. PLoS ONE |
| title | Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice. |
| title_full | Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice. |
| title_fullStr | Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice. |
| title_full_unstemmed | Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice. |
| title_short | Hair-Cell Mechanotransduction Persists in TRP Channel Knockout Mice. |
| title_sort | hair cell mechanotransduction persists in trp channel knockout mice |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155577&type=printable |
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