Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER

A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2...

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Main Authors: Hapsari Mahatmi, Agus Setiyono, Retno Damayanti Soejoedono, Fachriyan Hasmi Pasaribu
Format: Article
Language:English
Published: Universitas Udayana 2007-12-01
Series:Jurnal Veteriner
Subjects:
Online Access:http://ojs.unud.ac.id/index.php/jvet/article/view/3213
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author Hapsari Mahatmi
Agus Setiyono
Retno Damayanti Soejoedono
Fachriyan Hasmi Pasaribu
author_facet Hapsari Mahatmi
Agus Setiyono
Retno Damayanti Soejoedono
Fachriyan Hasmi Pasaribu
author_sort Hapsari Mahatmi
collection DOAJ
description A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.
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spelling doaj.art-b01056c92d604f2b89078cc95c7c14d52022-12-21T22:25:47ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652007-12-01843047Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVERHapsari MahatmiAgus SetiyonoRetno Damayanti SoejoedonoFachriyan Hasmi PasaribuA study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.http://ojs.unud.ac.id/index.php/jvet/article/view/3213Q fever, Coxiella burnetii, ruminant. nested-PCR
spellingShingle Hapsari Mahatmi
Agus Setiyono
Retno Damayanti Soejoedono
Fachriyan Hasmi Pasaribu
Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER
Jurnal Veteriner
Q fever, Coxiella burnetii, ruminant. nested-PCR
title Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER
title_full Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER
title_fullStr Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER
title_full_unstemmed Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER
title_short Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER
title_sort deteksi coxiella burnetii penyebab q fever pada sapi domba dan kambing di bogor dan bali detection of coxella burnetii the causal agent of q fever
topic Q fever, Coxiella burnetii, ruminant. nested-PCR
url http://ojs.unud.ac.id/index.php/jvet/article/view/3213
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