Overexpression and ELISA-based detection of asprosin in cultured cells and mice

Overexpression and ELISA-based detection of asprosin in cultured cells and mice

Summary: The unreliability of commercial recombinant asprosin preparations and variability between asprosin detection assays have proven to be a bottleneck in experimental interpretation. This protocol describes the use of viral vectors and expression plasmid for overexpression and secretion of huma...

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Bibliographic Details
Main Authors:	Ila Mishra, Atul R. Chopra
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Series:	STAR Protocols
Subjects:	Metabolism Molecular biology Gene expression Antibody Neuroscience Molecular/Chemical probes
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722006426

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