RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger
The studies found that strains of micromycete Aspergillus niger L-4 and B-3, producers of citric acid, can synthesize hydrolytic enzymes with invertase activity with a deep cultivation method on a sugar-mineral medium and medium based on starch hydrolysate. The most preferred regimes for conducting...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Russian Academy of Sciences, V.M. Gorbatov Federal Research Center for Food Systems
2018-03-01
|
| Series: | Пищевые системы |
| Subjects: | |
| Online Access: | https://www.fsjour.com/jour/article/view/5 |
| _version_ | 1826573010029510656 |
|---|---|
| author | Anastasia A. Printseva Natalya Yu Sharova Tatyana V. Vybornova |
| author_facet | Anastasia A. Printseva Natalya Yu Sharova Tatyana V. Vybornova |
| author_sort | Anastasia A. Printseva |
| collection | DOAJ |
| description | The studies found that strains of micromycete Aspergillus niger L-4 and B-3, producers of citric acid, can synthesize hydrolytic enzymes with invertase activity with a deep cultivation method on a sugar-mineral medium and medium based on starch hydrolysate. The most preferred regimes for conducting the biotechnological process are: the age of the inoculated mycelium is 24 h, the fermentation temperature for the sugar-mineral medium is32 °C and for starch hydrolysate is29 °C.For the Aspergillus niger strain L-4, the extracellular invertase activity for 120 h of the biotechnological process as a result of the fermentation of the sugar-mineral medium was (0,847 ± 0,068) u/cm3 of the native solution, and for strain B-3 — (0,966 ± 0,077) u/cm3 of the native solution, respectively.During fermentation of the medium, based on starch hydrolysate, extracellular invertase activity for strain L-4 was (1,379 ± 0,097) u/cm3 of native solution, and for strain B-3 — (1,597 ± 0,144) u/cm3 of native solution, respectively.The strains of the fungus Aspergillus niger L-4 and B-3 when cultivated on a sugar-mineral medium and medium, based on starch hydrolysate, have the ability to synthesize enzymes with invertase activity, the invertase activity of strain B-3 at the end of the process higher. The obtained data can be applied in further researches to develop a technology for the production of citric acid and invertase in one biotechnological process. |
| first_indexed | 2024-04-10T02:37:09Z |
| format | Article |
| id | doaj.art-b011e6d467e641d5baf2e4021f694a2f |
| institution | Directory Open Access Journal |
| issn | 2618-9771 2618-7272 |
| language | Russian |
| last_indexed | 2025-03-14T12:36:06Z |
| publishDate | 2018-03-01 |
| publisher | Russian Academy of Sciences, V.M. Gorbatov Federal Research Center for Food Systems |
| record_format | Article |
| series | Пищевые системы |
| spelling | doaj.art-b011e6d467e641d5baf2e4021f694a2f2025-03-02T09:22:25ZrusRussian Academy of Sciences, V.M. Gorbatov Federal Research Center for Food SystemsПищевые системы2618-97712618-72722018-03-0111192310.21323/2618-9771-2018-1-1-19-235RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus nigerAnastasia A. Printseva0Natalya Yu Sharova1Tatyana V. Vybornova2All-Russian Research institute for Food Additives —Branch of V.M. Gorbatov Federal Research Center for Food Systems of RASAll-Russian Research institute for Food Additives —Branch of V.M. Gorbatov Federal Research Center for Food Systems of RASAll-Russian Research institute for Food Additives —Branch of V.M. Gorbatov Federal Research Center for Food Systems of RASThe studies found that strains of micromycete Aspergillus niger L-4 and B-3, producers of citric acid, can synthesize hydrolytic enzymes with invertase activity with a deep cultivation method on a sugar-mineral medium and medium based on starch hydrolysate. The most preferred regimes for conducting the biotechnological process are: the age of the inoculated mycelium is 24 h, the fermentation temperature for the sugar-mineral medium is32 °C and for starch hydrolysate is29 °C.For the Aspergillus niger strain L-4, the extracellular invertase activity for 120 h of the biotechnological process as a result of the fermentation of the sugar-mineral medium was (0,847 ± 0,068) u/cm3 of the native solution, and for strain B-3 — (0,966 ± 0,077) u/cm3 of the native solution, respectively.During fermentation of the medium, based on starch hydrolysate, extracellular invertase activity for strain L-4 was (1,379 ± 0,097) u/cm3 of native solution, and for strain B-3 — (1,597 ± 0,144) u/cm3 of native solution, respectively.The strains of the fungus Aspergillus niger L-4 and B-3 when cultivated on a sugar-mineral medium and medium, based on starch hydrolysate, have the ability to synthesize enzymes with invertase activity, the invertase activity of strain B-3 at the end of the process higher. The obtained data can be applied in further researches to develop a technology for the production of citric acid and invertase in one biotechnological process.https://www.fsjour.com/jour/article/view/5citric acidproduceraspergillus nigerinvertase activityhydrolysatestarch |
| spellingShingle | Anastasia A. Printseva Natalya Yu Sharova Tatyana V. Vybornova RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger Пищевые системы citric acid producer aspergillus niger invertase activity hydrolysate starch |
| title | RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger |
| title_full | RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger |
| title_fullStr | RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger |
| title_full_unstemmed | RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger |
| title_short | RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger |
| title_sort | research of invertase activity when changing the parameters of the fermentation process sugar mineral medium and hydrolysate of starch by the micromycete aspergillus niger |
| topic | citric acid producer aspergillus niger invertase activity hydrolysate starch |
| url | https://www.fsjour.com/jour/article/view/5 |
| work_keys_str_mv | AT anastasiaaprintseva researchofinvertaseactivitywhenchangingtheparametersofthefermentationprocesssugarmineralmediumandhydrolysateofstarchbythemicromyceteaspergillusniger AT natalyayusharova researchofinvertaseactivitywhenchangingtheparametersofthefermentationprocesssugarmineralmediumandhydrolysateofstarchbythemicromyceteaspergillusniger AT tatyanavvybornova researchofinvertaseactivitywhenchangingtheparametersofthefermentationprocesssugarmineralmediumandhydrolysateofstarchbythemicromyceteaspergillusniger |