Characterization and Stability of the Antimony-Quercetin Complex
Purpose: Quercetin is a flavonoid known for its therapeutic properties and for forming complexes. Although the antimony-quercetin (SbQ) complex has been produced before, no previous exploration of its characteristics has been published in literature. Thus, this study aimed to characterize this compl...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2019-08-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-23028 |
| _version_ | 1819140216557928448 |
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| author | Valcilaine Teixeira Barbosa Janaína Barros de Menezes Josué Carinhanha Caldas Santos Maria Lysete de Assis Bastos João Xavier de Araújo-Júnior Ticiano Gomes do Nascimento Irinaldo Diniz Basílio-Júnior Luciano Aparecido Meireles Grillo Camila Braga Dornelas |
| author_facet | Valcilaine Teixeira Barbosa Janaína Barros de Menezes Josué Carinhanha Caldas Santos Maria Lysete de Assis Bastos João Xavier de Araújo-Júnior Ticiano Gomes do Nascimento Irinaldo Diniz Basílio-Júnior Luciano Aparecido Meireles Grillo Camila Braga Dornelas |
| author_sort | Valcilaine Teixeira Barbosa |
| collection | DOAJ |
| description | Purpose: Quercetin is a flavonoid known for its therapeutic properties and for forming complexes. Although the antimony-quercetin (SbQ) complex has been produced before, no previous exploration of its characteristics has been published in literature. Thus, this study aimed to characterize this complex, assess its stability and investigate its complexation site through its antibacterial activity. Methods: The SbQ complex was synthetized using Sb(III) potassium tartrate trihydrate and quercetin anhydrous (1:1) (v/v) as a solution and dried using three methods: rotaevaporation, lyophilization and spray drying. The material, in solution, was analyzed by UV-vis and fluorimetry; and, in the powder, by X-ray diffraction (XRD), both scanning electronic and fluorescence microscopy and infrared spectroscopy (FT-IR). Antimicrobial activity was evaluated via broth microdilution. Results: UV-vis exhibited a shoulder peak at 291 nm indicating metal chelation at C-ring of quercetin and confirmed 1:1 stoichiometry. Spectrofluorimetry showed an increase of intensity with the complex formation with an emission band (525 nm). After drying, XRD and SEM indicated loss of crystallinity and a difference in shape and size of the complex compared to its precursors. FT-IR suggested by a shift of frequency of the carbonyl group (1661 cm-1) that the quercetin bond to antimony by the C-3, followed by positions C-5 and C-4 carbonyl, which has been confirmed by MIC through the structure-activity relationship of the antibacterial activity of quercetin. Conclusion: These results provided a characterization of SbQ complex with the confirmation of its binding site, working as a guide for future studies involving this complex. |
| first_indexed | 2024-12-22T11:35:02Z |
| format | Article |
| id | doaj.art-b012ccd0756a411883a52a250e2c0eed |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-22T11:35:02Z |
| publishDate | 2019-08-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-b012ccd0756a411883a52a250e2c0eed2022-12-21T18:27:28ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082019-08-019343243810.15171/apb.2019.051apb-23028Characterization and Stability of the Antimony-Quercetin ComplexValcilaine Teixeira Barbosa0Janaína Barros de Menezes1Josué Carinhanha Caldas Santos2Maria Lysete de Assis Bastos3João Xavier de Araújo-Júnior4Ticiano Gomes do Nascimento5Irinaldo Diniz Basílio-Júnior6Luciano Aparecido Meireles Grillo7Camila Braga Dornelas8Departamento de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Departamento de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Instituto de Química e Biotecnologia, Universidade Federal de Alagoas.Departamento de Enfermagem, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Departamento de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Departamento de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Departamento de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Departamento de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Departamento de Farmácia, Escola de Enfermagem e Farmácia, Universidade Federal de Alagoas.Purpose: Quercetin is a flavonoid known for its therapeutic properties and for forming complexes. Although the antimony-quercetin (SbQ) complex has been produced before, no previous exploration of its characteristics has been published in literature. Thus, this study aimed to characterize this complex, assess its stability and investigate its complexation site through its antibacterial activity. Methods: The SbQ complex was synthetized using Sb(III) potassium tartrate trihydrate and quercetin anhydrous (1:1) (v/v) as a solution and dried using three methods: rotaevaporation, lyophilization and spray drying. The material, in solution, was analyzed by UV-vis and fluorimetry; and, in the powder, by X-ray diffraction (XRD), both scanning electronic and fluorescence microscopy and infrared spectroscopy (FT-IR). Antimicrobial activity was evaluated via broth microdilution. Results: UV-vis exhibited a shoulder peak at 291 nm indicating metal chelation at C-ring of quercetin and confirmed 1:1 stoichiometry. Spectrofluorimetry showed an increase of intensity with the complex formation with an emission band (525 nm). After drying, XRD and SEM indicated loss of crystallinity and a difference in shape and size of the complex compared to its precursors. FT-IR suggested by a shift of frequency of the carbonyl group (1661 cm-1) that the quercetin bond to antimony by the C-3, followed by positions C-5 and C-4 carbonyl, which has been confirmed by MIC through the structure-activity relationship of the antibacterial activity of quercetin. Conclusion: These results provided a characterization of SbQ complex with the confirmation of its binding site, working as a guide for future studies involving this complex.https://apb.tbzmed.ac.ir/PDF/apb-23028AntimonyBinding sitesFlavonoidsQuercetin |
| spellingShingle | Valcilaine Teixeira Barbosa Janaína Barros de Menezes Josué Carinhanha Caldas Santos Maria Lysete de Assis Bastos João Xavier de Araújo-Júnior Ticiano Gomes do Nascimento Irinaldo Diniz Basílio-Júnior Luciano Aparecido Meireles Grillo Camila Braga Dornelas Characterization and Stability of the Antimony-Quercetin Complex Advanced Pharmaceutical Bulletin Antimony Binding sites Flavonoids Quercetin |
| title | Characterization and Stability of the Antimony-Quercetin Complex |
| title_full | Characterization and Stability of the Antimony-Quercetin Complex |
| title_fullStr | Characterization and Stability of the Antimony-Quercetin Complex |
| title_full_unstemmed | Characterization and Stability of the Antimony-Quercetin Complex |
| title_short | Characterization and Stability of the Antimony-Quercetin Complex |
| title_sort | characterization and stability of the antimony quercetin complex |
| topic | Antimony Binding sites Flavonoids Quercetin |
| url | https://apb.tbzmed.ac.ir/PDF/apb-23028 |
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