| Summary: | Oyster (Crassostrea gigas) is a marine bivalve mollusk widely
distributed in coastal areas, and have been long widely used in industrial
resources. Several studies demonstrated that fermented oyster (FO) extract
attribute to bone health, but whether administration of FO play as an endocrine
disruptor has not been studied. Therefore, in the present study, we investigated
the effect of FO on the endocrine system in vitro and
in vivo. As the results of the competitive estrogen
receptor (ER) and androgen receptor (AR) binding affinities, FO was not combined
with ER-α, ER-β, and AR. However, 17β-estradiol and
testosterone, used as positive control, were interacted with ER and AR,
respectively. Meanwhile, oral administration of 100 mg/kg and 200 mg/kg of FO
doesn’t have any harmful effect on the body weight, androgen-dependent
sex accessory organs, estrogen-dependent-sex accessory organs, kidney, and liver
in immature rats. In addition, FO supplementation has no effect on the serum
levels of luteinizing hormone (LH), follicle stimulating hormone (FSH),
testosterone, and 17β-estradiol. However, the relative weight of
androgen- and estrogen-dependent organs were significantly increased by
subcutaneously injection of 4.0 mg/kg of testosterone propionate (TP) and by
orally administration of 1.0 μg of 17α-ethynyl estradiol (EE) in
immature male and female rats, respectively. Furthermore, TP and EE
administration markedly decreased the serum LH and FSH levels, which are similar
those of mature Sprague-Dawley (SD) rat. Furthermore, the testosterone and
17β-estradiol levels were significantly enhanced in TP and EE-treated
immature rats. Taken together, our findings showed that FO does not interact
with ER and AR, suggesting consequentially FO does not play as a ligand for ER
and AR. Furthermore, oral administration of FO did not act as an endocrine
disruptor including androgenic activity, estrogenic activity, and abnormal
levels of sex hormone, indicating FO may ensure the safety on endocrine system
to develop dietary supplement for bone health.
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