Direct RAPD evaluation of bacteria without conventional DNA extraction

The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों: Welington Luiz Araújo, Derlene Attili de Angellis, João Lúcio Azevedo
स्वरूप: लेख
भाषा:English
प्रकाशित: Instituto de Tecnologia do Paraná (Tecpar) 2004-07-01
श्रृंखला:Brazilian Archives of Biology and Technology
विषय:
ऑनलाइन पहुंच:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006