Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak
Abstract Background Coccidiosis caused by Eimeria zuernii (Eimeriidae: Coccidia) represents a significant economic threat to the bovine industry. Understanding the evolutionary and genetic biology of E. zuernii can assist in new interaction developments for the prevention and control of this protozo...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-023-05925-8 |
| _version_ | 1797453760396525568 |
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| author | Xuan Zhou Zhao Wang Pengchen Zhu Xiaobin Gu Ran He Jing Xu Bo Jing Lidan Wang Shun Chen Yue Xie |
| author_facet | Xuan Zhou Zhao Wang Pengchen Zhu Xiaobin Gu Ran He Jing Xu Bo Jing Lidan Wang Shun Chen Yue Xie |
| author_sort | Xuan Zhou |
| collection | DOAJ |
| description | Abstract Background Coccidiosis caused by Eimeria zuernii (Eimeriidae: Coccidia) represents a significant economic threat to the bovine industry. Understanding the evolutionary and genetic biology of E. zuernii can assist in new interaction developments for the prevention and control of this protozoosis. Methods We defined the evolutionary and genetic characteristics of E. zuernii by sequencing the complete mitogenome and analyzing the genetic diversity and population structure of 51 isolates collected from eight yak breeding parks in China. Results The 6176-bp mitogenome of E. zuernii was linear and encoded typical mitochondrial contents of apicomplexan parasites, including three protein-coding genes [PCGs; cytochrome c oxidase subunits I and III (cox1 and cox3), and cytochrome b (cytb)], seven fragmented small subunit (SSU) and 12 fragmented large subunit (LSU) rRNAs. Genome-wide comparative and evolutionary analyses showed cytb and cox3 to be the most and least conserved Eimeria PCGs, respectively, and placed E. zuernii more closely related to Eimeria mephitidis than other Eimeria species. Furthermore, cox1-based genetic structure defined 24 haplotypes of E. zuernii with high haplotype diversities and low nucleotide diversities across eight geographic populations, supporting a low genetic structure and rapid evolutionary rate as well as a previous expansion event among E. zuernii populations. Conclusions To our knowledge, this is the first study presenting the phylogeny, genetic diversity, and population structure of the yak E. zuernii, and such information, together with its mitogenomic data, should contribute to a better understanding of the genetic and evolutionary biological studies of apicomplexan parasites in bovines. Graphical Abstract |
| first_indexed | 2024-03-09T15:27:31Z |
| format | Article |
| id | doaj.art-b0150952946c45fb9f6066adeed12119 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-09T15:27:31Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-b0150952946c45fb9f6066adeed121192023-11-26T12:29:08ZengBMCParasites & Vectors1756-33052023-09-0116111110.1186/s13071-023-05925-8Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yakXuan Zhou0Zhao Wang1Pengchen Zhu2Xiaobin Gu3Ran He4Jing Xu5Bo Jing6Lidan Wang7Shun Chen8Yue Xie9Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Preventive Veterinary Medicine, Sichuan Agricultural UniversityDepartment of Parasitology, College of Veterinary Medicine, Sichuan Agricultural UniversityAbstract Background Coccidiosis caused by Eimeria zuernii (Eimeriidae: Coccidia) represents a significant economic threat to the bovine industry. Understanding the evolutionary and genetic biology of E. zuernii can assist in new interaction developments for the prevention and control of this protozoosis. Methods We defined the evolutionary and genetic characteristics of E. zuernii by sequencing the complete mitogenome and analyzing the genetic diversity and population structure of 51 isolates collected from eight yak breeding parks in China. Results The 6176-bp mitogenome of E. zuernii was linear and encoded typical mitochondrial contents of apicomplexan parasites, including three protein-coding genes [PCGs; cytochrome c oxidase subunits I and III (cox1 and cox3), and cytochrome b (cytb)], seven fragmented small subunit (SSU) and 12 fragmented large subunit (LSU) rRNAs. Genome-wide comparative and evolutionary analyses showed cytb and cox3 to be the most and least conserved Eimeria PCGs, respectively, and placed E. zuernii more closely related to Eimeria mephitidis than other Eimeria species. Furthermore, cox1-based genetic structure defined 24 haplotypes of E. zuernii with high haplotype diversities and low nucleotide diversities across eight geographic populations, supporting a low genetic structure and rapid evolutionary rate as well as a previous expansion event among E. zuernii populations. Conclusions To our knowledge, this is the first study presenting the phylogeny, genetic diversity, and population structure of the yak E. zuernii, and such information, together with its mitogenomic data, should contribute to a better understanding of the genetic and evolutionary biological studies of apicomplexan parasites in bovines. Graphical Abstracthttps://doi.org/10.1186/s13071-023-05925-8Yak coccidiosisEimeria zuerniiMtDNAGenetic relationshipsPopulation structure |
| spellingShingle | Xuan Zhou Zhao Wang Pengchen Zhu Xiaobin Gu Ran He Jing Xu Bo Jing Lidan Wang Shun Chen Yue Xie Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak Parasites & Vectors Yak coccidiosis Eimeria zuernii MtDNA Genetic relationships Population structure |
| title | Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak |
| title_full | Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak |
| title_fullStr | Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak |
| title_full_unstemmed | Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak |
| title_short | Eimeria zuernii (Eimeriidae: Coccidia): mitochondrial genome and genetic diversity in the Chinese yak |
| title_sort | eimeria zuernii eimeriidae coccidia mitochondrial genome and genetic diversity in the chinese yak |
| topic | Yak coccidiosis Eimeria zuernii MtDNA Genetic relationships Population structure |
| url | https://doi.org/10.1186/s13071-023-05925-8 |
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