Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro
Cell’s microenvironment has been shown to exert influence on cell behavior. In particular, matrix-cell interactions strongly impact cell morphology and function. The purpose of this study was to analyze the influence of different culture substrate materials on phenotype and functional properties of...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/5/675 |
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| author | Chiara Emma Campiglio Marina Figliuzzi Sara Silvani Matteo Tironi Sara Conti Federica Boschetti Andrea Remuzzi |
| author_facet | Chiara Emma Campiglio Marina Figliuzzi Sara Silvani Matteo Tironi Sara Conti Federica Boschetti Andrea Remuzzi |
| author_sort | Chiara Emma Campiglio |
| collection | DOAJ |
| description | Cell’s microenvironment has been shown to exert influence on cell behavior. In particular, matrix-cell interactions strongly impact cell morphology and function. The purpose of this study was to analyze the influence of different culture substrate materials on phenotype and functional properties of lung epithelial adenocarcinoma (A549) cells. A549 cells were seeded onto two different biocompatible, commercially available substrates: a polyester coverslip (Thermanox™ Coverslips), that was used as cell culture plate control, and a polydimethylsiloxane membrane (PDMS, Elastosil<sup>®</sup> Film) investigated in this study as alternative material for A549 cells culture. The two substrates influenced cell morphology and the actin cytoskeleton organization. Further, the Yes-associated protein (YAP) and its transcriptional coactivator PDZ-binding motif (TAZ) were translocated to the nucleus in A549 cells cultured on polyester substrate, yet it remained mostly cytosolic in cells on PDMS substrate. By SEM analysis, we observed that cells grown on Elastosil<sup>®</sup> Film maintained an alveolar Type II cell morphology. Immunofluorescence staining for surfactant-C revealing a high expression of surfactant-C in cells cultured on Elastosil<sup>®</sup> Film, but not in cells cultured on Thermanox™ Coverslips. A549 cells grown onto Elastosil<sup>®</sup> Film exhibited morphology and functionality that suggest retainment of alveolar epithelial Type II phenotype, while A549 cells grown onto conventional plastic substrates acquired an alveolar Type I phenotype. |
| first_indexed | 2024-03-10T11:47:27Z |
| format | Article |
| id | doaj.art-b0161f333bbb42f5a8d1c799f9e7a1a5 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T11:47:27Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b0161f333bbb42f5a8d1c799f9e7a1a52023-11-21T17:59:13ZengMDPI AGBiomolecules2218-273X2021-04-0111567510.3390/biom11050675Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In VitroChiara Emma Campiglio0Marina Figliuzzi1Sara Silvani2Matteo Tironi3Sara Conti4Federica Boschetti5Andrea Remuzzi6Department of Management, Information and Production Engineering, University of Bergamo, 24044 Dalmine, ItalyDepartment of Biomedical Engineering, Istituto di Ricerche Farmacologiche Mario Negri-IRCCS, 24126 Bergamo, ItalyDepartment of Biomedical Engineering, Istituto di Ricerche Farmacologiche Mario Negri-IRCCS, 24126 Bergamo, ItalyDepartment of Biomedical Engineering, Istituto di Ricerche Farmacologiche Mario Negri-IRCCS, 24126 Bergamo, ItalyDepartment of Molecular Medicine, Istituto di Ricerche Farmacologiche Mario Negri-IRCCS, 24126 Bergamo, ItalyDepartment of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, 20131 Milan, ItalyDepartment of Management, Information and Production Engineering, University of Bergamo, 24044 Dalmine, ItalyCell’s microenvironment has been shown to exert influence on cell behavior. In particular, matrix-cell interactions strongly impact cell morphology and function. The purpose of this study was to analyze the influence of different culture substrate materials on phenotype and functional properties of lung epithelial adenocarcinoma (A549) cells. A549 cells were seeded onto two different biocompatible, commercially available substrates: a polyester coverslip (Thermanox™ Coverslips), that was used as cell culture plate control, and a polydimethylsiloxane membrane (PDMS, Elastosil<sup>®</sup> Film) investigated in this study as alternative material for A549 cells culture. The two substrates influenced cell morphology and the actin cytoskeleton organization. Further, the Yes-associated protein (YAP) and its transcriptional coactivator PDZ-binding motif (TAZ) were translocated to the nucleus in A549 cells cultured on polyester substrate, yet it remained mostly cytosolic in cells on PDMS substrate. By SEM analysis, we observed that cells grown on Elastosil<sup>®</sup> Film maintained an alveolar Type II cell morphology. Immunofluorescence staining for surfactant-C revealing a high expression of surfactant-C in cells cultured on Elastosil<sup>®</sup> Film, but not in cells cultured on Thermanox™ Coverslips. A549 cells grown onto Elastosil<sup>®</sup> Film exhibited morphology and functionality that suggest retainment of alveolar epithelial Type II phenotype, while A549 cells grown onto conventional plastic substrates acquired an alveolar Type I phenotype.https://www.mdpi.com/2218-273X/11/5/675cell functioncell morphologymechanobiologysubstrate propertieslung epithelial cells |
| spellingShingle | Chiara Emma Campiglio Marina Figliuzzi Sara Silvani Matteo Tironi Sara Conti Federica Boschetti Andrea Remuzzi Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro Biomolecules cell function cell morphology mechanobiology substrate properties lung epithelial cells |
| title | Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro |
| title_full | Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro |
| title_fullStr | Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro |
| title_full_unstemmed | Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro |
| title_short | Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro |
| title_sort | influence of culture substrates on morphology and function of pulmonary alveolar cells in vitro |
| topic | cell function cell morphology mechanobiology substrate properties lung epithelial cells |
| url | https://www.mdpi.com/2218-273X/11/5/675 |
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