Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primer
The objective of this study was to determine time of neural fold fusion at dorsal mid line neural axis after treatment 2-methoxyethanol(2-ME) to pregnant mouse during neurulation period and to observe relation process of point of neural fold fusion. Mice at 08:05gestational days treatment with 2-ME...
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| Format: | Article |
| Language: | English |
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Indonesian Biological Society
2012-02-01
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| Series: | Journal of Biological Researches |
| Subjects: | |
| Online Access: | http://berkalahayati.org/journal/48e84433 |
| _version_ | 1831679074677817344 |
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| author | Eko Prihiyantoro Win Darmanto Samekto Wibowo Mammed Sagi Sri Kadarsih Soedjono |
| author_facet | Eko Prihiyantoro Win Darmanto Samekto Wibowo Mammed Sagi Sri Kadarsih Soedjono |
| author_sort | Eko Prihiyantoro |
| collection | DOAJ |
| description | The objective of this study was to determine time of neural fold fusion at dorsal mid line neural axis after treatment 2-methoxyethanol(2-ME) to pregnant mouse during neurulation period and to observe relation process of point of neural fold fusion. Mice at 08:05gestational days treatment with 2-ME dose 7.5 mmol/kg bw on the other hand control group injected with aqua bidest. Pregnant miceat 08:12, 09:00, 09:12 was sacrificed by dislocation cervix. Embryo was collected after observe with dissecting microscope for externalmorphology and fixation in Bouin solution and buffer formalin for histological preparation and immunohistochemistry process. Resultshowed that there was failure of first point neural fold fusion at junction of perspective fore brain and mid brain. The failure of neuralfold fusion was caused by increasing apoptosis neuroepithelium. There were no relation process between first point of neural fold fusionand second point or another point of fusion. Failure of first point fusion not cause failure another point of fusion. Observation at 09:12gestational days showed that the only first point fusion was still open but formation of another part neural tube have finished. |
| first_indexed | 2024-12-20T05:12:02Z |
| format | Article |
| id | doaj.art-b0173158a6034a80b4d49a182acc4bb7 |
| institution | Directory Open Access Journal |
| issn | 0852-6834 2337-389X |
| language | English |
| last_indexed | 2024-12-20T05:12:02Z |
| publishDate | 2012-02-01 |
| publisher | Indonesian Biological Society |
| record_format | Article |
| series | Journal of Biological Researches |
| spelling | doaj.art-b0173158a6034a80b4d49a182acc4bb72022-12-21T19:52:14ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2012-02-0113216317210.23869/bphjbr.13.2.200811Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primerEko Prihiyantoro0Win Darmanto1Samekto Wibowo2Mammed Sagi3Sri Kadarsih Soedjono4Departemen Biologi Fakultas Sains dan Teknologi Universitas Airlangga ** Bagian Neurologi Fakultas Kedokteran Universitas Gadjah Mada *** Lab Histologi Fakultas Biologi Universitas Gadjah Mada **** Bagian Faal Fakultas Kedokteran Universitas Gadjah MadaDepartemen Biologi Fakultas Sains dan Teknologi Universitas Airlangga ** Bagian Neurologi Fakultas Kedokteran Universitas Gadjah Mada *** Lab Histologi Fakultas Biologi Universitas Gadjah Mada **** Bagian Faal Fakultas Kedokteran Universitas Gadjah MadaDepartemen Biologi Fakultas Sains dan Teknologi Universitas Airlangga ** Bagian Neurologi Fakultas Kedokteran Universitas Gadjah Mada *** Lab Histologi Fakultas Biologi Universitas Gadjah Mada **** Bagian Faal Fakultas Kedokteran Universitas Gadjah MadaDepartemen Biologi Fakultas Sains dan Teknologi Universitas Airlangga ** Bagian Neurologi Fakultas Kedokteran Universitas Gadjah Mada *** Lab Histologi Fakultas Biologi Universitas Gadjah Mada **** Bagian Faal Fakultas Kedokteran Universitas Gadjah MadaDepartemen Biologi Fakultas Sains dan Teknologi Universitas Airlangga ** Bagian Neurologi Fakultas Kedokteran Universitas Gadjah Mada *** Lab Histologi Fakultas Biologi Universitas Gadjah Mada **** Bagian Faal Fakultas Kedokteran Universitas Gadjah MadaThe objective of this study was to determine time of neural fold fusion at dorsal mid line neural axis after treatment 2-methoxyethanol(2-ME) to pregnant mouse during neurulation period and to observe relation process of point of neural fold fusion. Mice at 08:05gestational days treatment with 2-ME dose 7.5 mmol/kg bw on the other hand control group injected with aqua bidest. Pregnant miceat 08:12, 09:00, 09:12 was sacrificed by dislocation cervix. Embryo was collected after observe with dissecting microscope for externalmorphology and fixation in Bouin solution and buffer formalin for histological preparation and immunohistochemistry process. Resultshowed that there was failure of first point neural fold fusion at junction of perspective fore brain and mid brain. The failure of neuralfold fusion was caused by increasing apoptosis neuroepithelium. There were no relation process between first point of neural fold fusionand second point or another point of fusion. Failure of first point fusion not cause failure another point of fusion. Observation at 09:12gestational days showed that the only first point fusion was still open but formation of another part neural tube have finished.http://berkalahayati.org/journal/48e84433NTDsexcencephally2-ME |
| spellingShingle | Eko Prihiyantoro Win Darmanto Samekto Wibowo Mammed Sagi Sri Kadarsih Soedjono Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primer Journal of Biological Researches NTDs excencephally 2-ME |
| title | Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primer |
| title_full | Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primer |
| title_fullStr | Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primer |
| title_full_unstemmed | Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primer |
| title_short | Gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2-ME yang bertepatan dengan masa neurulasi primer |
| title_sort | gangguan pembentukan atap bumbung neural embrio mencit akibat induksi 2 me yang bertepatan dengan masa neurulasi primer |
| topic | NTDs excencephally 2-ME |
| url | http://berkalahayati.org/journal/48e84433 |
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