Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study
Volume-stable collagen matrices (VSCM) are conductive for the connective tissue upon soft tissue augmentation. Considering that collagen has osteoconductive properties, we have investigated the possibility that the VSCM also consolidates with the newly formed bone. To this end, we covered nine rat c...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2227-9059/9/7/732 |
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| author | Karol Alí Apaza Alccayhuaman Stefan Tangl Stéphane Blouin Markus A. Hartmann Patrick Heimel Ulrike Kuchler Jung-Seok Lee Reinhard Gruber |
| author_facet | Karol Alí Apaza Alccayhuaman Stefan Tangl Stéphane Blouin Markus A. Hartmann Patrick Heimel Ulrike Kuchler Jung-Seok Lee Reinhard Gruber |
| author_sort | Karol Alí Apaza Alccayhuaman |
| collection | DOAJ |
| description | Volume-stable collagen matrices (VSCM) are conductive for the connective tissue upon soft tissue augmentation. Considering that collagen has osteoconductive properties, we have investigated the possibility that the VSCM also consolidates with the newly formed bone. To this end, we covered nine rat calvaria circular defects with a VSCM. After four weeks, histology, histomorphometry, quantitative backscattered electron imaging, and microcomputed tomography were performed. We report that the overall pattern of mineralization inside the VSCM was heterogeneous. Histology revealed, apart from the characteristic woven bone formation, areas of round-shaped hypertrophic chondrocyte-like cells surrounded by a mineralized extracellular matrix. Quantitative backscattered electron imaging confirmed the heterogenous mineralization occurring within the VSCM. Histomorphometry found new bone to be 0.7 mm<sup>2</sup> (0.01 min; 2.4 max), similar to the chondrogenic mineralized extracellular matrix with 0.7 mm<sup>2</sup> (0.0 min; 4.2 max). Microcomputed tomography showed the overall mineralized tissue in the defect to be 1.6 mm<sup>3</sup> (min 0.0; max 13.3). These findings suggest that in a rat cranial defect, VSCM has a limited and heterogeneous capacity to support intramembranous bone formation but may allow the formation of bone via the endochondral route. |
| first_indexed | 2024-03-09T04:55:21Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T04:55:21Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-b01845f10b334031b8c8de0b3f6b38172023-12-03T13:06:51ZengMDPI AGBiomedicines2227-90592021-06-019773210.3390/biomedicines9070732Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot StudyKarol Alí Apaza Alccayhuaman0Stefan Tangl1Stéphane Blouin2Markus A. Hartmann3Patrick Heimel4Ulrike Kuchler5Jung-Seok Lee6Reinhard Gruber7Department of Oral Biology, Dental School, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, AustriaKarl Donath Laboratory for Hard Tissue and Biomaterial Research, Division of Oral Surgery, School of Dentistry, Medical University of Vienna, 1090 Vienna, Austria1st Medical Department, Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling, Hanusch Hospital, 1140 Vienna, Austria1st Medical Department, Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling, Hanusch Hospital, 1140 Vienna, AustriaKarl Donath Laboratory for Hard Tissue and Biomaterial Research, Division of Oral Surgery, School of Dentistry, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Surgery, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, Dental School, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, AustriaDepartment of Oral Biology, Dental School, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, AustriaVolume-stable collagen matrices (VSCM) are conductive for the connective tissue upon soft tissue augmentation. Considering that collagen has osteoconductive properties, we have investigated the possibility that the VSCM also consolidates with the newly formed bone. To this end, we covered nine rat calvaria circular defects with a VSCM. After four weeks, histology, histomorphometry, quantitative backscattered electron imaging, and microcomputed tomography were performed. We report that the overall pattern of mineralization inside the VSCM was heterogeneous. Histology revealed, apart from the characteristic woven bone formation, areas of round-shaped hypertrophic chondrocyte-like cells surrounded by a mineralized extracellular matrix. Quantitative backscattered electron imaging confirmed the heterogenous mineralization occurring within the VSCM. Histomorphometry found new bone to be 0.7 mm<sup>2</sup> (0.01 min; 2.4 max), similar to the chondrogenic mineralized extracellular matrix with 0.7 mm<sup>2</sup> (0.0 min; 4.2 max). Microcomputed tomography showed the overall mineralized tissue in the defect to be 1.6 mm<sup>3</sup> (min 0.0; max 13.3). These findings suggest that in a rat cranial defect, VSCM has a limited and heterogeneous capacity to support intramembranous bone formation but may allow the formation of bone via the endochondral route.https://www.mdpi.com/2227-9059/9/7/732bone regenerationvolume-stable collagen matrixcollagen matrixhistologyqBEI |
| spellingShingle | Karol Alí Apaza Alccayhuaman Stefan Tangl Stéphane Blouin Markus A. Hartmann Patrick Heimel Ulrike Kuchler Jung-Seok Lee Reinhard Gruber Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study Biomedicines bone regeneration volume-stable collagen matrix collagen matrix histology qBEI |
| title | Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study |
| title_full | Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study |
| title_fullStr | Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study |
| title_full_unstemmed | Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study |
| title_short | Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study |
| title_sort | osteoconductive properties of a volume stable collagen matrix in rat calvaria defects a pilot study |
| topic | bone regeneration volume-stable collagen matrix collagen matrix histology qBEI |
| url | https://www.mdpi.com/2227-9059/9/7/732 |
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