Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication
The effect of the alkaloid 1-(phenyl)-N’-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU). Both methodologies suggested th...
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| Format: | Article |
| Language: | English |
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Sciendo
2015-03-01
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| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/acph-2015-0004 |
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| author | Santos Patricia Regina Formagio Anelise S. Nazari Filho Benedito Prado Dias Nakamura Celso Vataru Sarragiotto Maria Helena Ueda-Nakamura Tania |
| author_facet | Santos Patricia Regina Formagio Anelise S. Nazari Filho Benedito Prado Dias Nakamura Celso Vataru Sarragiotto Maria Helena Ueda-Nakamura Tania |
| author_sort | Santos Patricia Regina |
| collection | DOAJ |
| description | The effect of the alkaloid 1-(phenyl)-N’-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU). Both methodologies suggested that the mode of action was avoidance of infection progression in the host cell. The compound was able to prevent CPE and PFU formation when the cells were pretreated with PMC for 24 h prior to PV infection. In addition, when the alkaloid was continuously maintained in the infected cultures, the spread of PV to adjacent cells was impaired. The pre-exposure and post-exposure prophylactic applications are possible situations in which an anti-PV drug might be used. Future studies are needed to elucidate the PMC mode of action and verify the feasibility of PMC use in vivo. No antipicornavirus agent is currently approved for clinical use |
| first_indexed | 2024-12-17T23:48:07Z |
| format | Article |
| id | doaj.art-b01ae5a3efa14b1283ee96ca644ec839 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2025-02-16T13:42:28Z |
| publishDate | 2015-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-b01ae5a3efa14b1283ee96ca644ec8392025-02-02T09:57:48ZengSciendoActa Pharmaceutica1846-95582015-03-01651758110.1515/acph-2015-0004acph-2015-0004Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replicationSantos Patricia Regina0Formagio Anelise S. Nazari1Filho Benedito Prado Dias2Nakamura Celso Vataru3Sarragiotto Maria Helena4Ueda-Nakamura Tania5Post-Graduate Program in Pharmaceutical Sciences State University of Maringá 87020-900, Maringá, PR, BrazilPost-Graduate Program in Chemistry State University of Maringá 87020-900, Maringá, PR, BrazilPost-Graduate Program in Pharmaceutical Sciences State University of Maringá 87020-900, Maringá, PR, BrazilPost-Graduate Program in Pharmaceutical Sciences State University of Maringá 87020-900, Maringá, PR, BrazilPost-Graduate Program in Chemistry State University of Maringá 87020-900, Maringá, PR, BrazilPost-Graduate Program in Pharmaceutical Sciences State University of Maringá 87020-900, Maringá, PR, BrazilThe effect of the alkaloid 1-(phenyl)-N’-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU). Both methodologies suggested that the mode of action was avoidance of infection progression in the host cell. The compound was able to prevent CPE and PFU formation when the cells were pretreated with PMC for 24 h prior to PV infection. In addition, when the alkaloid was continuously maintained in the infected cultures, the spread of PV to adjacent cells was impaired. The pre-exposure and post-exposure prophylactic applications are possible situations in which an anti-PV drug might be used. Future studies are needed to elucidate the PMC mode of action and verify the feasibility of PMC use in vivo. No antipicornavirus agent is currently approved for clinical usehttps://doi.org/10.1515/acph-2015-00041-(phenyl)-n’-(4-methoxybenzylidene)-9h-pyrido[ 3,4-b]indole-3-carbohydrazideb-carbolinepoliovirusantiviral activity |
| spellingShingle | Santos Patricia Regina Formagio Anelise S. Nazari Filho Benedito Prado Dias Nakamura Celso Vataru Sarragiotto Maria Helena Ueda-Nakamura Tania Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication Acta Pharmaceutica 1-(phenyl)-n’-(4-methoxybenzylidene)-9h-pyrido[ 3,4-b]indole-3-carbohydrazide b-carboline poliovirus antiviral activity |
| title | Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication |
| title_full | Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication |
| title_fullStr | Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication |
| title_full_unstemmed | Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication |
| title_short | Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication |
| title_sort | effect of 1 phenyl n 4 methoxybenzylidene 9h pyrido 3 4 b indole 3 carbohydrazide on in vitro poliovirus replication |
| topic | 1-(phenyl)-n’-(4-methoxybenzylidene)-9h-pyrido[ 3,4-b]indole-3-carbohydrazide b-carboline poliovirus antiviral activity |
| url | https://doi.org/10.1515/acph-2015-0004 |
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