A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity
CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs). Type III systems exhibit a robust immune response that can lead to the extinction of a phage population, a feat coordinated by a mu...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/45393 |
| _version_ | 1811180556205948928 |
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| author | Lucy Chou-Zheng Asma Hatoum-Aslan |
| author_facet | Lucy Chou-Zheng Asma Hatoum-Aslan |
| author_sort | Lucy Chou-Zheng |
| collection | DOAJ |
| description | CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs). Type III systems exhibit a robust immune response that can lead to the extinction of a phage population, a feat coordinated by a multi-subunit effector complex that destroys invading DNA and RNA. Here, we demonstrate that a model type III system in Staphylococcus epidermidis relies upon the activities of two degradosome-associated nucleases, PNPase and RNase J2, to mount a successful defense. Genetic, molecular, and biochemical analyses reveal that PNPase promotes crRNA maturation, and both nucleases are required for efficient clearance of phage-derived nucleic acids. Furthermore, functional assays show that RNase J2 is essential for immunity against diverse mobile genetic elements originating from plasmid and phage. Altogether, our observations reveal the evolution of a critical collaboration between two nucleic acid degrading machines which ensures cell survival when faced with phage attack. |
| first_indexed | 2024-04-11T09:05:18Z |
| format | Article |
| id | doaj.art-b01c4303d5e04b5ca9513e9bbc52f829 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:05:18Z |
| publishDate | 2019-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b01c4303d5e04b5ca9513e9bbc52f8292022-12-22T04:32:39ZengeLife Sciences Publications LtdeLife2050-084X2019-04-01810.7554/eLife.45393A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunityLucy Chou-Zheng0Asma Hatoum-Aslan1https://orcid.org/0000-0003-2395-8900Department of Biological Sciences, The University of Alabama, Tuscaloosa, United StatesDepartment of Biological Sciences, The University of Alabama, Tuscaloosa, United StatesCRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs). Type III systems exhibit a robust immune response that can lead to the extinction of a phage population, a feat coordinated by a multi-subunit effector complex that destroys invading DNA and RNA. Here, we demonstrate that a model type III system in Staphylococcus epidermidis relies upon the activities of two degradosome-associated nucleases, PNPase and RNase J2, to mount a successful defense. Genetic, molecular, and biochemical analyses reveal that PNPase promotes crRNA maturation, and both nucleases are required for efficient clearance of phage-derived nucleic acids. Furthermore, functional assays show that RNase J2 is essential for immunity against diverse mobile genetic elements originating from plasmid and phage. Altogether, our observations reveal the evolution of a critical collaboration between two nucleic acid degrading machines which ensures cell survival when faced with phage attack.https://elifesciences.org/articles/45393Staphylococcus epidermidisbacteriophageCRISPR-Cas10type III CRISPRdegradosome |
| spellingShingle | Lucy Chou-Zheng Asma Hatoum-Aslan A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity eLife Staphylococcus epidermidis bacteriophage CRISPR-Cas10 type III CRISPR degradosome |
| title | A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity |
| title_full | A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity |
| title_fullStr | A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity |
| title_full_unstemmed | A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity |
| title_short | A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity |
| title_sort | type iii a crispr cas system employs degradosome nucleases to ensure robust immunity |
| topic | Staphylococcus epidermidis bacteriophage CRISPR-Cas10 type III CRISPR degradosome |
| url | https://elifesciences.org/articles/45393 |
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