Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio
Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/7/1000 |
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| author | Omar Ulises Cruz-Ramírez Lucía Margarita Valenzuela-Salas Alberto Blanco-Salazar José Antonio Rodríguez-Arenas Paris A. Mier-Maldonado Juan Carlos García-Ramos Nina Bogdanchikova Alexey Pestryakov Yanis Toledano-Magaña |
| author_facet | Omar Ulises Cruz-Ramírez Lucía Margarita Valenzuela-Salas Alberto Blanco-Salazar José Antonio Rodríguez-Arenas Paris A. Mier-Maldonado Juan Carlos García-Ramos Nina Bogdanchikova Alexey Pestryakov Yanis Toledano-Magaña |
| author_sort | Omar Ulises Cruz-Ramírez |
| collection | DOAJ |
| description | Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their cytotoxic effect on primary mouse cultures, and their lethal dose on BALB/c mice. The evaluated AgNP formulations have a composition within the ranges Ag: 1.14–1.32% <i>w</i>/<i>w</i>, PVP: 19.6–24.5% and H<sub>2</sub>O: 74.2–79.2% with predominant spherical shape within an average size range of 16–30 nm according to transmission electron microscopy (TEM). All formulations assessed increase mitochondrial ROS concentration and induce apoptosis as the leading death pathway on HCT-15 cells. Except for AgNP1, the growth inhibition potency of AgNP formulations of human colon tumor cancer cells (HCT-15) is 34.5 times higher than carboplatin, one of the first-line chemotherapy agents. Nevertheless, 5–10% of necrotic events, even at the lower concentration evaluated, were observed. The cytotoxic selectivity was confirmed by evaluating the cytotoxic effect on aorta, spleen, heart, liver, and kidney primary cultures from BALB/c mice. Despite the cytotoxic effects observed in vitro, the lethal dose and histopathological analysis showed the low toxicity of these formulations (all of them on Category 4 of the Globally Harmonized System of Classification and Labelling of Chemicals) and minor damage observed on analyzed organs. The results provide an additional example of the rational design of safety nanomaterials with antitumor potency and urge further experiments to complete the preclinical studies for these AgNP formulations. |
| first_indexed | 2024-03-10T09:28:58Z |
| format | Article |
| id | doaj.art-b01c4a5f749c4e25b9b5ae83e9635cae |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T09:28:58Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-b01c4a5f749c4e25b9b5ae83e9635cae2023-11-22T04:40:30ZengMDPI AGPharmaceutics1999-49232021-07-01137100010.3390/pharmaceutics13071000Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] RatioOmar Ulises Cruz-Ramírez0Lucía Margarita Valenzuela-Salas1Alberto Blanco-Salazar2José Antonio Rodríguez-Arenas3Paris A. Mier-Maldonado4Juan Carlos García-Ramos5Nina Bogdanchikova6Alexey Pestryakov7Yanis Toledano-Magaña8Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada 22860, MexicoFacultad de Ciencias de la Salud Unidad Valle de las Palmas, Universidad Autónoma de Baja California, Tijuana 22260, MexicoPrograma de Maestría y Doctorado en Ciencias e Ingeniería, Facultad de Ciencias, Universidad Autónoma de Baja California, Ensenada 22860, MexicoPrograma de Maestría y Doctorado en Ciencias e Ingeniería, Facultad de Ciencias, Universidad Autónoma de Baja California, Ensenada 22860, MexicoFacultad de Ciencias de la Salud Unidad Valle de las Palmas, Universidad Autónoma de Baja California, Tijuana 22260, MexicoEscuela de Ciencias de la Salud Unidad Valle Dorado, Universidad Autónoma de Baja California, Ensenada 22890, MexicoCentro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada 22860, MexicoResearch School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, RussiaEscuela de Ciencias de la Salud Unidad Valle Dorado, Universidad Autónoma de Baja California, Ensenada 22890, MexicoSilver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their cytotoxic effect on primary mouse cultures, and their lethal dose on BALB/c mice. The evaluated AgNP formulations have a composition within the ranges Ag: 1.14–1.32% <i>w</i>/<i>w</i>, PVP: 19.6–24.5% and H<sub>2</sub>O: 74.2–79.2% with predominant spherical shape within an average size range of 16–30 nm according to transmission electron microscopy (TEM). All formulations assessed increase mitochondrial ROS concentration and induce apoptosis as the leading death pathway on HCT-15 cells. Except for AgNP1, the growth inhibition potency of AgNP formulations of human colon tumor cancer cells (HCT-15) is 34.5 times higher than carboplatin, one of the first-line chemotherapy agents. Nevertheless, 5–10% of necrotic events, even at the lower concentration evaluated, were observed. The cytotoxic selectivity was confirmed by evaluating the cytotoxic effect on aorta, spleen, heart, liver, and kidney primary cultures from BALB/c mice. Despite the cytotoxic effects observed in vitro, the lethal dose and histopathological analysis showed the low toxicity of these formulations (all of them on Category 4 of the Globally Harmonized System of Classification and Labelling of Chemicals) and minor damage observed on analyzed organs. The results provide an additional example of the rational design of safety nanomaterials with antitumor potency and urge further experiments to complete the preclinical studies for these AgNP formulations.https://www.mdpi.com/1999-4923/13/7/1000AgNPsantitumor activitycolon cancerHCT-15cytotoxic selectivity[metal]/[coating agent] ratio |
| spellingShingle | Omar Ulises Cruz-Ramírez Lucía Margarita Valenzuela-Salas Alberto Blanco-Salazar José Antonio Rodríguez-Arenas Paris A. Mier-Maldonado Juan Carlos García-Ramos Nina Bogdanchikova Alexey Pestryakov Yanis Toledano-Magaña Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio Pharmaceutics AgNPs antitumor activity colon cancer HCT-15 cytotoxic selectivity [metal]/[coating agent] ratio |
| title | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
| title_full | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
| title_fullStr | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
| title_full_unstemmed | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
| title_short | Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio |
| title_sort | antitumor activity against human colorectal adenocarcinoma of silver nanoparticles influence of ag pvp ratio |
| topic | AgNPs antitumor activity colon cancer HCT-15 cytotoxic selectivity [metal]/[coating agent] ratio |
| url | https://www.mdpi.com/1999-4923/13/7/1000 |
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