Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i>
The NADP-malic enzyme (NADP-ME) catalyzes the reversible decarboxylation of L-malate to produce pyruvate, CO<sub>2</sub>, and NADPH in the presence of a bivalent cation. In addition, this enzyme plays crucial roles in plant developmental and environment responses, especially for the plas...
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MDPI AG
2021-09-01
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| author | Zhibin Wen Yulan Wang Chunlan Xia Yuhui Zhang Hongxiang Zhang |
| author_facet | Zhibin Wen Yulan Wang Chunlan Xia Yuhui Zhang Hongxiang Zhang |
| author_sort | Zhibin Wen |
| collection | DOAJ |
| description | The NADP-malic enzyme (NADP-ME) catalyzes the reversible decarboxylation of L-malate to produce pyruvate, CO<sub>2</sub>, and NADPH in the presence of a bivalent cation. In addition, this enzyme plays crucial roles in plant developmental and environment responses, especially for the plastidic isoform. However, this isoform is less studied in C<sub>3</sub>–C<sub>4</sub> intermediate species under drought and salt stresses than in C<sub>3</sub> and C<sub>4</sub> species. In the present study, we characterized <i>SaNADP-ME4</i> from the intermediate woody desert species <i>Salsola laricifolia</i>. <i>SaNADP-ME4</i> encoded a protein of 646 amino acids, which was found to be located in the chloroplasts based on confocal imaging. Quantitative real-time PCR analysis showed that <i>SaNADP-ME4</i> was highly expressed in leaves, followed by stems and roots, and <i>SaNADP-ME4</i> expression was improved and reached its maximum under the 200 mm mannitol and 100 mm NaCl treatments, respectively. <i>Arabidopsis</i> overexpressing <i>SaNADP-ME4</i> showed increased root length and fresh weight under mannitol and salt stress conditions at the seedling stage. In the adult stage, <i>SaNADP-ME4</i> could alleviate the decreased in chlorophyll contents and PSII photochemical efficiency, as well as improve the expression of superoxide dismutase, peroxidase, and pyrroline-5-carboxylate synthase genes to enhance reactive oxygen species scavenging capability and proline levels. Our results suggest that <i>SaNADP-ME4</i> overexpression in <i>Arabidopsis</i> increases drought and salt stress resistance. |
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| spelling | doaj.art-b01ede5d514144009dc03aa7fcb86cc92023-11-22T14:52:21ZengMDPI AGPlants2223-77472021-09-01109182710.3390/plants10091827Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i>Zhibin Wen0Yulan Wang1Chunlan Xia2Yuhui Zhang3Hongxiang Zhang4State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, ChinaThe NADP-malic enzyme (NADP-ME) catalyzes the reversible decarboxylation of L-malate to produce pyruvate, CO<sub>2</sub>, and NADPH in the presence of a bivalent cation. In addition, this enzyme plays crucial roles in plant developmental and environment responses, especially for the plastidic isoform. However, this isoform is less studied in C<sub>3</sub>–C<sub>4</sub> intermediate species under drought and salt stresses than in C<sub>3</sub> and C<sub>4</sub> species. In the present study, we characterized <i>SaNADP-ME4</i> from the intermediate woody desert species <i>Salsola laricifolia</i>. <i>SaNADP-ME4</i> encoded a protein of 646 amino acids, which was found to be located in the chloroplasts based on confocal imaging. Quantitative real-time PCR analysis showed that <i>SaNADP-ME4</i> was highly expressed in leaves, followed by stems and roots, and <i>SaNADP-ME4</i> expression was improved and reached its maximum under the 200 mm mannitol and 100 mm NaCl treatments, respectively. <i>Arabidopsis</i> overexpressing <i>SaNADP-ME4</i> showed increased root length and fresh weight under mannitol and salt stress conditions at the seedling stage. In the adult stage, <i>SaNADP-ME4</i> could alleviate the decreased in chlorophyll contents and PSII photochemical efficiency, as well as improve the expression of superoxide dismutase, peroxidase, and pyrroline-5-carboxylate synthase genes to enhance reactive oxygen species scavenging capability and proline levels. Our results suggest that <i>SaNADP-ME4</i> overexpression in <i>Arabidopsis</i> increases drought and salt stress resistance.https://www.mdpi.com/2223-7747/10/9/1827<i>SaNADP-ME4</i>C<sub>3</sub>–C<sub>4</sub> intermediate speciesabiotic stress<i>Salsola laricifolia</i> |
| spellingShingle | Zhibin Wen Yulan Wang Chunlan Xia Yuhui Zhang Hongxiang Zhang Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i> Plants <i>SaNADP-ME4</i> C<sub>3</sub>–C<sub>4</sub> intermediate species abiotic stress <i>Salsola laricifolia</i> |
| title | Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i> |
| title_full | Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i> |
| title_fullStr | Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i> |
| title_full_unstemmed | Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i> |
| title_short | Chloroplastic <i>SaNADP-ME4</i> of C<sub>3</sub>–C<sub>4</sub> Woody Desert Species <i>Salsola laricifolia</i> Confers Drought and Salt Stress Resistance to <i>Arabidopsis</i> |
| title_sort | chloroplastic i sanadp me4 i of c sub 3 sub c sub 4 sub woody desert species i salsola laricifolia i confers drought and salt stress resistance to i arabidopsis i |
| topic | <i>SaNADP-ME4</i> C<sub>3</sub>–C<sub>4</sub> intermediate species abiotic stress <i>Salsola laricifolia</i> |
| url | https://www.mdpi.com/2223-7747/10/9/1827 |
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