Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry
Seminal plasma proteins have recently been reported to play a significant role as valuable materials for understanding male reproductive biology, identifying causes of fertility problems, and developing reproductive biomarkers. Proteomic analysis of seminal plasma holds promise in advancing the unde...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1174078/full |
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| author | Podjana Wattananit Yodying Yingchutrakul Kornchai Kornkaewrat Sittidet Mahasawangkul Sittiruk Roytrakul Anuchai Pinyopummin |
| author_facet | Podjana Wattananit Yodying Yingchutrakul Kornchai Kornkaewrat Sittidet Mahasawangkul Sittiruk Roytrakul Anuchai Pinyopummin |
| author_sort | Podjana Wattananit |
| collection | DOAJ |
| description | Seminal plasma proteins have recently been reported to play a significant role as valuable materials for understanding male reproductive biology, identifying causes of fertility problems, and developing reproductive biomarkers. Proteomic analysis of seminal plasma holds promise in advancing the understanding of male Asian elephant reproductive biology. This study aims to explore seminal plasma proteins of Asian elephants and their probable functions to provide fundamental information about male reproduction in this species. The protein solution from pooled seminal plasma from 10 bulls (a total of 33 ejaculates) was digested into peptides and identified using LC-MS/MS. Out of 986 proteins, 597 were mapped and matched with 58 species in UniProt databases, including Elephas maximus. These mapped proteins were mostly involved in binding function, catalytic activity, cellular process, and metabolic process. Only 29 mapped proteins were recognized to be related in reproductive process, mainly associated in spermatogenesis and sperm capacitation. Additionally, several seminal plasma proteins related to fertility or semen quality in other mammals were also found in Asian elephant semen, such as keratin type I, aldose reductase, thrombospondon-1, fibronectin 1, platelet-activating factor acetyl hydrolase, mannosidase, and semenogelin-2. This discovery clearly reveals the beneficial protein profile in seminal plasma of the Asian elephant and serves as a crucial step in investigating infertility and poor semen quality in this valuable species. |
| first_indexed | 2024-03-11T23:10:49Z |
| format | Article |
| id | doaj.art-b0213bfa23bb4c83a8ff526de235696a |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-11T23:10:49Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b0213bfa23bb4c83a8ff526de235696a2023-09-21T09:01:47ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-09-011010.3389/fvets.2023.11740781174078Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometryPodjana Wattananit0Yodying Yingchutrakul1Kornchai Kornkaewrat2Sittidet Mahasawangkul3Sittiruk Roytrakul4Anuchai Pinyopummin5Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, ThailandNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, ThailandFaculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, ThailandThe National Elephant Institute, The Forest Industry Organization, Lampang, ThailandNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, ThailandFaculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, ThailandSeminal plasma proteins have recently been reported to play a significant role as valuable materials for understanding male reproductive biology, identifying causes of fertility problems, and developing reproductive biomarkers. Proteomic analysis of seminal plasma holds promise in advancing the understanding of male Asian elephant reproductive biology. This study aims to explore seminal plasma proteins of Asian elephants and their probable functions to provide fundamental information about male reproduction in this species. The protein solution from pooled seminal plasma from 10 bulls (a total of 33 ejaculates) was digested into peptides and identified using LC-MS/MS. Out of 986 proteins, 597 were mapped and matched with 58 species in UniProt databases, including Elephas maximus. These mapped proteins were mostly involved in binding function, catalytic activity, cellular process, and metabolic process. Only 29 mapped proteins were recognized to be related in reproductive process, mainly associated in spermatogenesis and sperm capacitation. Additionally, several seminal plasma proteins related to fertility or semen quality in other mammals were also found in Asian elephant semen, such as keratin type I, aldose reductase, thrombospondon-1, fibronectin 1, platelet-activating factor acetyl hydrolase, mannosidase, and semenogelin-2. This discovery clearly reveals the beneficial protein profile in seminal plasma of the Asian elephant and serves as a crucial step in investigating infertility and poor semen quality in this valuable species.https://www.frontiersin.org/articles/10.3389/fvets.2023.1174078/fullAsian elephantseminal plasmaproteinproteomic analysismale reproduction |
| spellingShingle | Podjana Wattananit Yodying Yingchutrakul Kornchai Kornkaewrat Sittidet Mahasawangkul Sittiruk Roytrakul Anuchai Pinyopummin Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry Frontiers in Veterinary Science Asian elephant seminal plasma protein proteomic analysis male reproduction |
| title | Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry |
| title_full | Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry |
| title_fullStr | Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry |
| title_full_unstemmed | Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry |
| title_short | Non-targeted proteomic analysis of Asian elephant (Elephas maximus) seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry |
| title_sort | non targeted proteomic analysis of asian elephant elephas maximus seminal plasma using an in solution digestion technique and liquid chromatography tandem mass spectrometry |
| topic | Asian elephant seminal plasma protein proteomic analysis male reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1174078/full |
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