Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry
The need for greener extraction procedures that are quick and efficient has prompted the evolution of pressurized hot water extraction (PHWE). Here, the extraction of flavonoids from Bidens pilosa was demonstrated using PHWE at 50°C, 100°C and 150°C. The extracts were analyzed on UPLC-qTOF-MS/MS and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-04-01
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| Series: | CyTA - Journal of Food |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19476337.2016.1230151 |
| _version_ | 1818491153133076480 |
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| author | Sefeter Gbashi Patrick Njobeh Paul Steenkamp Ntakadzeni Madala |
| author_facet | Sefeter Gbashi Patrick Njobeh Paul Steenkamp Ntakadzeni Madala |
| author_sort | Sefeter Gbashi |
| collection | DOAJ |
| description | The need for greener extraction procedures that are quick and efficient has prompted the evolution of pressurized hot water extraction (PHWE). Here, the extraction of flavonoids from Bidens pilosa was demonstrated using PHWE at 50°C, 100°C and 150°C. The extracts were analyzed on UPLC-qTOF-MS/MS and 28 flavonoids of different classes were identified. Further analysis of the data using principal component analysis revealed differential distribution patterns of the identified molecules. In overall, the extraction yield increased proportionately with increasing temperature. It can thus be deduced that PHWE is an excellent extraction method of flavonoids from plant tissues. Again, this study reiterates B. pilosa as a rich source of flavonoids. |
| first_indexed | 2024-12-10T17:26:56Z |
| format | Article |
| id | doaj.art-b02611fbc0134072bfd242830fec5695 |
| institution | Directory Open Access Journal |
| issn | 1947-6337 1947-6345 |
| language | English |
| last_indexed | 2024-12-10T17:26:56Z |
| publishDate | 2017-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj.art-b02611fbc0134072bfd242830fec56952022-12-22T01:39:49ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452017-04-0115217118010.1080/19476337.2016.12301511230151Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometrySefeter Gbashi0Patrick Njobeh1Paul Steenkamp2Ntakadzeni Madala3University of JohannesburgUniversity of JohannesburgBiosciences, Natural Products and Agroprocessing GroupUniversity of JohannesburgThe need for greener extraction procedures that are quick and efficient has prompted the evolution of pressurized hot water extraction (PHWE). Here, the extraction of flavonoids from Bidens pilosa was demonstrated using PHWE at 50°C, 100°C and 150°C. The extracts were analyzed on UPLC-qTOF-MS/MS and 28 flavonoids of different classes were identified. Further analysis of the data using principal component analysis revealed differential distribution patterns of the identified molecules. In overall, the extraction yield increased proportionately with increasing temperature. It can thus be deduced that PHWE is an excellent extraction method of flavonoids from plant tissues. Again, this study reiterates B. pilosa as a rich source of flavonoids.http://dx.doi.org/10.1080/19476337.2016.1230151Pressurized hot waterBidens pilosaflavonoids and principal component analysis |
| spellingShingle | Sefeter Gbashi Patrick Njobeh Paul Steenkamp Ntakadzeni Madala Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry CyTA - Journal of Food Pressurized hot water Bidens pilosa flavonoids and principal component analysis |
| title | Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry |
| title_full | Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry |
| title_fullStr | Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry |
| title_full_unstemmed | Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry |
| title_short | Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry |
| title_sort | pressurized hot water extraction and chemometric fingerprinting of flavonoids from bidens pilosa by uplc tandem mass spectrometry |
| topic | Pressurized hot water Bidens pilosa flavonoids and principal component analysis |
| url | http://dx.doi.org/10.1080/19476337.2016.1230151 |
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