Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system
The manganese peroxidase (MnP) can degrade multiple mycotoxins including deoxynivalenol (DON) efficiently; however, the lignin components abundant in foods and feeds were discovered to interfere with DON catalysis. Herein, using MnP from Ceriporiopsis subvermispora (CsMnP) as a model, it was demonst...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324002057 |
| _version_ | 1797261857628618752 |
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| author | Xiaoyun Su Shuai Wang Xiaolu Wang Wangli Ji Honglian Zhang Tao Tu Nina Hakulinen Huiying Luo Bin Yao Wei Zhang Huoqing Huang |
| author_facet | Xiaoyun Su Shuai Wang Xiaolu Wang Wangli Ji Honglian Zhang Tao Tu Nina Hakulinen Huiying Luo Bin Yao Wei Zhang Huoqing Huang |
| author_sort | Xiaoyun Su |
| collection | DOAJ |
| description | The manganese peroxidase (MnP) can degrade multiple mycotoxins including deoxynivalenol (DON) efficiently; however, the lignin components abundant in foods and feeds were discovered to interfere with DON catalysis. Herein, using MnP from Ceriporiopsis subvermispora (CsMnP) as a model, it was demonstrated that desired catalysis of DON, but not futile reactions with lignin, in the reaction systems containing feeds could be achieved by engineering MnP and supplementing with a boosting reactant. Specifically, two successive strategies (including the fusion of CsMnP to a DON-recognizing ScFv and identification of glutathione as a specific targeting enhancer) were combined to overcome the lignin competition, which together resulted into elevation of the degradation rate from 2.5% to as high as 82.7% in the feeds. The method to construct a targeting MnP and fortify it with an additional enhancer could be similarly applied to catalyze the many other mycotoxins with yet unknown responsive biocatalysts. |
| first_indexed | 2024-03-07T22:55:34Z |
| format | Article |
| id | doaj.art-b02646a6d9be49979af95fc0ac00c165 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-24T23:47:53Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-b02646a6d9be49979af95fc0ac00c1652024-03-15T04:42:48ZengElsevierEcotoxicology and Environmental Safety0147-65132024-03-01273116130Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione systemXiaoyun Su0Shuai Wang1Xiaolu Wang2Wangli Ji3Honglian Zhang4Tao Tu5Nina Hakulinen6Huiying Luo7 Bin Yao8Wei Zhang9Huoqing Huang10State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100193, China; Correspondence to: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No.12 South Zhongguancun St., Haidian District, Beijing 100081, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100193, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, No.12 South Zhongguancun St., Haidian District, Beijing 100081, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100193, ChinaDepartment of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu FIN-80101, FinlandState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100193, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, No.12 South Zhongguancun St., Haidian District, Beijing 100081, China; Correspondence to: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100193, China; Correspondence to: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.The manganese peroxidase (MnP) can degrade multiple mycotoxins including deoxynivalenol (DON) efficiently; however, the lignin components abundant in foods and feeds were discovered to interfere with DON catalysis. Herein, using MnP from Ceriporiopsis subvermispora (CsMnP) as a model, it was demonstrated that desired catalysis of DON, but not futile reactions with lignin, in the reaction systems containing feeds could be achieved by engineering MnP and supplementing with a boosting reactant. Specifically, two successive strategies (including the fusion of CsMnP to a DON-recognizing ScFv and identification of glutathione as a specific targeting enhancer) were combined to overcome the lignin competition, which together resulted into elevation of the degradation rate from 2.5% to as high as 82.7% in the feeds. The method to construct a targeting MnP and fortify it with an additional enhancer could be similarly applied to catalyze the many other mycotoxins with yet unknown responsive biocatalysts.http://www.sciencedirect.com/science/article/pii/S0147651324002057Manganese peroxidaseDeoxynivalenolScFvGlutathioneMycotoxin detoxification |
| spellingShingle | Xiaoyun Su Shuai Wang Xiaolu Wang Wangli Ji Honglian Zhang Tao Tu Nina Hakulinen Huiying Luo Bin Yao Wei Zhang Huoqing Huang Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system Ecotoxicology and Environmental Safety Manganese peroxidase Deoxynivalenol ScFv Glutathione Mycotoxin detoxification |
| title | Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system |
| title_full | Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system |
| title_fullStr | Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system |
| title_full_unstemmed | Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system |
| title_short | Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system |
| title_sort | targeting deoxynivalenol for degradation by a chimeric manganese peroxidase glutathione system |
| topic | Manganese peroxidase Deoxynivalenol ScFv Glutathione Mycotoxin detoxification |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324002057 |
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