A method for mechanized hybrid rice seed production using female sterile rice
Abstract Background The breeding and large-scale adoption of hybrid rice is an important achievement in modern agriculture. Mechanized seed production is urgently needed for widespread adoption of hybrid rice because it can compensate for the shortage of manual labor to meet the growing food demands...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-05-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12284-019-0296-8 |
| _version_ | 1828434151666089984 |
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| author | Yumei Xia Ning Tang Yuanyi Hu Ding Li Shuangcheng Li Xiaolan Bu Mulan Yu Shaowu Qi Yishan Yang Hongjin Zhu Chenying Cao Ping Li Longping Yuan Mengliang Cao |
| author_facet | Yumei Xia Ning Tang Yuanyi Hu Ding Li Shuangcheng Li Xiaolan Bu Mulan Yu Shaowu Qi Yishan Yang Hongjin Zhu Chenying Cao Ping Li Longping Yuan Mengliang Cao |
| author_sort | Yumei Xia |
| collection | DOAJ |
| description | Abstract Background The breeding and large-scale adoption of hybrid rice is an important achievement in modern agriculture. Mechanized seed production is urgently needed for widespread adoption of hybrid rice because it can compensate for the shortage of manual labor to meet the growing food demands in China. Results Here, we report the development of a mechanized hybrid rice seed production method using a female sterile rice. In this method, three closely linked gene expression cassettes were introduced into female sterile rice. The three expression cassettes are: 1) a rice female fertility gene expression cassette; 2) a pollen-lethal gene expression cassette; and 3) a red fluorescence protein gene expression cassette. During the self-fertilization process of a heterozygous transgenic rice plant, pollen grains carrying the transgene die off and cannot participate in fertilization; pollen grains not carrying a transgene can normally fertilize the female gamete, leading to fructification. By means of fluorescence-assisted sorting, homogeneous female sterile rice seeds are sorted out from other seeds carrying the transgene and are used for mechanized hybrid rice seed production; heterozygous seeds carrying the transgene can then be used in the multiplication of female sterile rice. Conclusions This technology solves the difficulty of multiplying female-sterile rice, allows for mechanized production of hybrid rice seed, and will prove especially valuable in systems using a mixed-planting, mixed-harvesting approach. Moreover, it uses transgenic technology that has not yet been employed in a seed production process in which the output is non-transgenic seeds. |
| first_indexed | 2024-12-10T18:46:14Z |
| format | Article |
| id | doaj.art-b02b56ea6b664bae92128492b53b724b |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-10T18:46:14Z |
| publishDate | 2019-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-b02b56ea6b664bae92128492b53b724b2022-12-22T01:37:30ZengSpringerOpenRice1939-84251939-84332019-05-0112111010.1186/s12284-019-0296-8A method for mechanized hybrid rice seed production using female sterile riceYumei Xia0Ning Tang1Yuanyi Hu2Ding Li3Shuangcheng Li4Xiaolan Bu5Mulan Yu6Shaowu Qi7Yishan Yang8Hongjin Zhu9Chenying Cao10Ping Li11Longping Yuan12Mengliang Cao13State Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterRice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterState Key Laboratory of Hybrid Rice, Longping Graduate School, Hunan University , Changsha, 410082, China/ Hunan Hybrid Rice Research CenterAbstract Background The breeding and large-scale adoption of hybrid rice is an important achievement in modern agriculture. Mechanized seed production is urgently needed for widespread adoption of hybrid rice because it can compensate for the shortage of manual labor to meet the growing food demands in China. Results Here, we report the development of a mechanized hybrid rice seed production method using a female sterile rice. In this method, three closely linked gene expression cassettes were introduced into female sterile rice. The three expression cassettes are: 1) a rice female fertility gene expression cassette; 2) a pollen-lethal gene expression cassette; and 3) a red fluorescence protein gene expression cassette. During the self-fertilization process of a heterozygous transgenic rice plant, pollen grains carrying the transgene die off and cannot participate in fertilization; pollen grains not carrying a transgene can normally fertilize the female gamete, leading to fructification. By means of fluorescence-assisted sorting, homogeneous female sterile rice seeds are sorted out from other seeds carrying the transgene and are used for mechanized hybrid rice seed production; heterozygous seeds carrying the transgene can then be used in the multiplication of female sterile rice. Conclusions This technology solves the difficulty of multiplying female-sterile rice, allows for mechanized production of hybrid rice seed, and will prove especially valuable in systems using a mixed-planting, mixed-harvesting approach. Moreover, it uses transgenic technology that has not yet been employed in a seed production process in which the output is non-transgenic seeds.http://link.springer.com/article/10.1186/s12284-019-0296-8Hybrid riceFemale sterilityMechanization of hybrid seed productionBreeding |
| spellingShingle | Yumei Xia Ning Tang Yuanyi Hu Ding Li Shuangcheng Li Xiaolan Bu Mulan Yu Shaowu Qi Yishan Yang Hongjin Zhu Chenying Cao Ping Li Longping Yuan Mengliang Cao A method for mechanized hybrid rice seed production using female sterile rice Rice Hybrid rice Female sterility Mechanization of hybrid seed production Breeding |
| title | A method for mechanized hybrid rice seed production using female sterile rice |
| title_full | A method for mechanized hybrid rice seed production using female sterile rice |
| title_fullStr | A method for mechanized hybrid rice seed production using female sterile rice |
| title_full_unstemmed | A method for mechanized hybrid rice seed production using female sterile rice |
| title_short | A method for mechanized hybrid rice seed production using female sterile rice |
| title_sort | method for mechanized hybrid rice seed production using female sterile rice |
| topic | Hybrid rice Female sterility Mechanization of hybrid seed production Breeding |
| url | http://link.springer.com/article/10.1186/s12284-019-0296-8 |
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