THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER
<span style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14.666666984558105px; font-style: italic; text-align: justify; background-color: #ddeaf4;">A study was conducted to apply reverse transcriptase-polymerase chain reaction (RT-PCR) technique for...
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| Format: | Article |
| Language: | English |
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Universitas Udayana
2008-03-01
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| Series: | Jurnal Veteriner |
| Subjects: | |
| Online Access: | http://ojs.unud.ac.id/index.php/jvet/article/view/3310 |
| _version_ | 1818024438035120128 |
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| author | I Nyoman Suartha I Gusti Ngurah Kade Mahardika Ida Ayu Sri Candra Dewi Ni Ketut Dias Nursanty Yosaphat L.S Kote Anita Dwi Handayani I Gusti Agung Ayu Suartini |
| author_facet | I Nyoman Suartha I Gusti Ngurah Kade Mahardika Ida Ayu Sri Candra Dewi Ni Ketut Dias Nursanty Yosaphat L.S Kote Anita Dwi Handayani I Gusti Agung Ayu Suartini |
| author_sort | I Nyoman Suartha |
| collection | DOAJ |
| description | <span style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14.666666984558105px; font-style: italic; text-align: justify; background-color: #ddeaf4;">A study was conducted to apply reverse transcriptase-polymerase chain reaction (RT-PCR) technique for the confirmative diagnosis of canine distemper in dogs. Twenty mongreal dogs with clinical symptoms of canine distemper were used in this study. The viral RNA was isolated from nasal swab using Trizol® and transcribed into cDNA using random primers 5’ACAGGATTGCTGAGGACCTAT 3’. The cDNA was amplified in one step RT-PCR using primers 5’-ACAGGATTGCTGAGGACCTAT-3’ (forward) and 5’- CAAGATAACCATGTACGGTGC-3’ (backward). A single band of 300 bp which was specific for canine distemper virus CDV) was detected in fifteen out of twenty samples. It is therefore evident that confirmative diagnostics of canine distemper disease can be established with RT-PCR technique.</span><div><span style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14.666666984558105px; font-style: italic; text-align: justify; background-color: #ddeaf4;"><br /></span></div> |
| first_indexed | 2024-12-10T04:00:13Z |
| format | Article |
| id | doaj.art-b02df6a3101c4825b0b402d2fbf0a432 |
| institution | Directory Open Access Journal |
| issn | 1411-8327 2477-5665 |
| language | English |
| last_indexed | 2024-12-10T04:00:13Z |
| publishDate | 2008-03-01 |
| publisher | Universitas Udayana |
| record_format | Article |
| series | Jurnal Veteriner |
| spelling | doaj.art-b02df6a3101c4825b0b402d2fbf0a4322022-12-22T02:03:00ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652008-03-01913144THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPERI Nyoman SuarthaI Gusti Ngurah Kade MahardikaIda Ayu Sri Candra DewiNi Ketut Dias NursantyYosaphat L.S KoteAnita Dwi HandayaniI Gusti Agung Ayu Suartini<span style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14.666666984558105px; font-style: italic; text-align: justify; background-color: #ddeaf4;">A study was conducted to apply reverse transcriptase-polymerase chain reaction (RT-PCR) technique for the confirmative diagnosis of canine distemper in dogs. Twenty mongreal dogs with clinical symptoms of canine distemper were used in this study. The viral RNA was isolated from nasal swab using Trizol® and transcribed into cDNA using random primers 5’ACAGGATTGCTGAGGACCTAT 3’. The cDNA was amplified in one step RT-PCR using primers 5’-ACAGGATTGCTGAGGACCTAT-3’ (forward) and 5’- CAAGATAACCATGTACGGTGC-3’ (backward). A single band of 300 bp which was specific for canine distemper virus CDV) was detected in fifteen out of twenty samples. It is therefore evident that confirmative diagnostics of canine distemper disease can be established with RT-PCR technique.</span><div><span style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14.666666984558105px; font-style: italic; text-align: justify; background-color: #ddeaf4;"><br /></span></div>http://ojs.unud.ac.id/index.php/jvet/article/view/3310diagnostics, canine distemper, RT-PCR |
| spellingShingle | I Nyoman Suartha I Gusti Ngurah Kade Mahardika Ida Ayu Sri Candra Dewi Ni Ketut Dias Nursanty Yosaphat L.S Kote Anita Dwi Handayani I Gusti Agung Ayu Suartini THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER Jurnal Veteriner diagnostics, canine distemper, RT-PCR |
| title | THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER |
| title_full | THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER |
| title_fullStr | THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER |
| title_full_unstemmed | THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER |
| title_short | THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER |
| title_sort | aplication of reverse transcriptase polymerase chain reaction for the diagnosis of canine distemper |
| topic | diagnostics, canine distemper, RT-PCR |
| url | http://ojs.unud.ac.id/index.php/jvet/article/view/3310 |
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